Construct: ORF TRCN0000489818
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021973.1_s317c1
- DNA Barcode:
- TATGATCCCCCGGGCGTATTTAGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ESR2 (2100)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489818
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001437.2 | 100% | 100% | |
| 2 | human | 2100 | ESR2 | estrogen receptor 2 | XM_017021079.1 | 100% | 100% | |
| 3 | human | 2100 | ESR2 | estrogen receptor 2 | XM_017021080.1 | 100% | 100% | |
| 4 | human | 2100 | ESR2 | estrogen receptor 2 | XM_017021081.1 | 100% | 100% | |
| 5 | human | 2100 | ESR2 | estrogen receptor 2 | XM_017021082.1 | 100% | 100% | |
| 6 | human | 2100 | ESR2 | estrogen receptor 2 | XM_017021083.1 | 100% | 100% | |
| 7 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001040275.1 | 90.3% | 88.9% | (many diffs) |
| 8 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001291712.2 | 90.3% | 88.9% | (many diffs) |
| 9 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001291723.1 | 90.3% | 88.9% | (many diffs) |
| 10 | human | 2100 | ESR2 | estrogen receptor 2 | XM_017021084.1 | 90.3% | 88.9% | (many diffs) |
| 11 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001214902.1 | 89.7% | 88.6% | (many diffs) |
| 12 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001271876.1 | 88.3% | 87.9% | 1406_1413delGGTCCTGT;1417delT;1422_1423ins177 |
| 13 | human | 2100 | ESR2 | estrogen receptor 2 | NM_001271877.1 | 82.8% | 82.8% | 952_953ins273 |
| 14 | human | 2100 | ESR2 | estrogen receptor 2 | NR_073497.1 | 76.9% | (many diffs) | |
| 15 | human | 2100 | ESR2 | estrogen receptor 2 | XR_001750187.1 | 53.3% | (many diffs) | |
| 16 | human | 2100 | ESR2 | estrogen receptor 2 | NR_073496.2 | 43.8% | (many diffs) | |
| 17 | mouse | 13983 | Esr2 | estrogen receptor 2 (beta) | NM_010157.3 | 81.6% | 85.6% | (many diffs) |
| 18 | mouse | 13983 | Esr2 | estrogen receptor 2 (beta) | NM_207707.1 | 79% | 82.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1659
- ORF length:
- 1590
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggatataaaa aactcaccat ctagccttaa ttctccttcc tcctacaact 121 gcagtcaatc catcttaccc ctggagcacg gctccatata cataccttcc tcctatgtag 181 acagccacca tgaatatcca gccatgacat tctatagccc tgctgtgatg aattacagca 241 ttcccagcaa tgtcactaac ttggaaggtg ggcctggtcg gcagaccaca agcccaaatg 301 tgttgtggcc aacacctggg cacctttctc ctttagtggt ccatcgccag ttatcacatc 361 tgtatgcgga acctcaaaag agtccctggt gtgaagcaag atcgctagaa cacaccttac 421 ctgtaaacag agagacactg aaaaggaagg ttagtgggaa ccgttgcgcc agccctgtta 481 ctggtccagg ttcaaagagg gatgctcact tctgcgctgt ctgcagcgat tacgcatcgg 541 gatatcacta tggagtctgg tcgtgtgaag gatgtaaggc cttttttaaa agaagcattc 601 aaggacataa tgattatatt tgtccagcta caaatcagtg tacaatcgat aaaaaccggc 661 gcaagagctg ccaggcctgc cgacttcgga agtgttacga agtgggaatg gtgaagtgtg 721 gctcccggag agagagatgt gggtaccgcc ttgtgcggag acagagaagt gccgacgagc 781 agctgcactg tgccggcaag gccaagagaa gtggcggcca cgcgccccga gtgcgggagc 841 tgctgctgga cgccctgagc cccgagcagc tagtgctcac cctcctggag gctgagccgc 901 cccatgtgct gatcagccgc cccagtgcgc ccttcaccga ggcctccatg atgatgtccc 961 tgaccaagtt ggccgacaag gagttggtac acatgatcag ctgggccaag aagattcccg 1021 gctttgtgga gctcagcctg ttcgaccaag tgcggctctt ggagagctgt tggatggagg 1081 tgttaatgat ggggctgatg tggcgctcaa ttgaccaccc cggcaagctc atctttgctc 1141 cagatcttgt tctggacagg gatgagggga aatgcgtaga aggaattctg gaaatctttg 1201 acatgctcct ggcaactact tcaaggtttc gagagttaaa actccaacac aaagaatatc 1261 tctgtgtcaa ggccatgatc ctGCTCAATT CCAGTATGTA CCCTCTGGTC ACAGCGACCC 1321 AGGATGCTGA CAGCAGCCGG AAGCTGGCTC ACTTGCTGAA CGCCGTGACC GATGCTTTGG 1381 TTTGGGTGAT TGCCAAGAGC GGCATCTCCT CCCAGCAGCA ATCCATGCGC CTGGCTAACC 1441 TCCTGATGCT CCTGTCCCAC GTCAGGCATG CGAGTAACAA GGGCATGGAA CATCTGCTCA 1501 ACATGAAGTG CAAAAATGTG GTCCCAGTGT ATGACCTGCT GCTGGAGATG CTGAATGCCC 1561 ACGTGCTTCG CGGGTGCAAG TCCTCCATCA CGGGGTCCGA GTGCAGCCCG GCAGAGGACA 1621 GTAAAAGCAA AGAGGGCTCC CAGAACCCAC AGTCTCAGTG AGACCCAGCT TTCTTGTACA 1681 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1741 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1801 AGGACGATAT GATCCCCCGG GCGTATTTAG CACGCGTTAA GTCgacaatc aacctctgga 1861 ttacaaaatt tgtgaaagat t