Construct: ORF TRCN0000489823

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021187.1_s317c1
DNA Barcode:
CTTGACCTCCTCCCAGTACTTTTG
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
NPR3 (4883)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489823
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4883 NPR3 natriuretic peptide receptor 3 NM_001204375.2 100% 100%
2 human 4883 NPR3 natriuretic peptide receptor 3 NM_000908.4 99.8% 99.6% 1426_1427insCAG
3 human 4883 NPR3 natriuretic peptide receptor 3 XM_017009492.2 92.4% 92.4% 768_769ins123
4 human 4883 NPR3 natriuretic peptide receptor 3 NM_001363652.2 58.6% 52.8% (many diffs)
5 human 4883 NPR3 natriuretic peptide receptor 3 NM_001204376.1 58.4% 52.4% (many diffs)
6 human 4883 NPR3 natriuretic peptide receptor 3 XM_011514047.2 57.2% 53.3% (many diffs)
7 human 4883 NPR3 natriuretic peptide receptor 3 NM_001364458.2 54.4% 53% (many diffs)
8 human 4883 NPR3 natriuretic peptide receptor 3 XM_011514049.3 52.1% 52.1% 0_1ins777
9 human 4883 NPR3 natriuretic peptide receptor 3 NM_001364460.2 50.8% 43.1% (many diffs)
10 human 4883 NPR3 natriuretic peptide receptor 3 XM_011514050.2 49.3% 47.3% (many diffs)
11 mouse 18162 Npr3 natriuretic peptide receptor 3 NM_008728.2 86.5% 90.7% (many diffs)
12 mouse 18162 Npr3 natriuretic peptide receptor 3 NM_001039181.1 86.3% 90.3% (many diffs)
13 mouse 18162 Npr3 natriuretic peptide receptor 3 XM_006520028.2 46.7% 49.3% (many diffs)
14 mouse 18162 Npr3 natriuretic peptide receptor 3 XM_006520030.3 46.7% 49.3% (many diffs)
15 mouse 18162 Npr3 natriuretic peptide receptor 3 XM_006520031.2 46.7% 49.3% (many diffs)
16 mouse 18162 Npr3 natriuretic peptide receptor 3 XM_017316493.1 46.7% 49.3% (many diffs)
17 mouse 18162 Npr3 natriuretic peptide receptor 3 NM_001286395.1 46.5% 48.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
1695
ORF length:
1623
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgccgtct ctgctggtgc tcactttctc cccgtgcgta ctactcggct
121gggcgttgct ggccggcggc accggtggcg gtggcgttgg cggcggcggc ggtggcgcgg
181gcataggcgg cggacgccag gagagagagg cgctgccgcc acagaagatc gaggtgctgg
241tgttactgcc ccaggatgac tcgtacttgt tttcactcac ccgggtgcgg ccggccatcg
301agtatgctct gcgcagcgtg gagggcaacg ggactgggag gcggcttctg ccgccgggca
361ctcgcttcca ggtggcttac gaggattcag actgtgggaa ccgtgcgctc ttcagcttgg
421tggaccgcgt ggcggcggcg cggggcgcca agccagacct tatcctgggg ccagtgtgcg
481agtatgcagc agcgccagtg gcccggcttg catcgcactg ggacctgccc atgctgtcgg
541ctggggcgct ggccgctggc ttccagcaca aggactctga gtactcgcac ctcacgcgcg
601tggcgcccgc ctacgccaag atgggcgaga tgatgctcgc cctgttccgc caccaccact
661ggagccgcgc tgcactggtc tacagcgacg acaagctgga gcggaactgc tacttcaccc
721tcgagggggt ccacgaggtc ttccaggagg agggtttgca cacgtccatc tacagtttcg
781acgagaccaa agacttggat ctggaagaca tcgtgcgcaa tatccaggcc agtgagagag
841tggtgatcat gtgtgcgagc agtgacacca tccggagcat catgctggtg gcgcacaggc
901atggcatgac cagtggagac tacgccttct tcaacattga gctcttcaac agctcttcct
961atggagatgg ctcatggaag agaggagaca aacacgactt tgaagctaag caagcatact
1021cgtccctcca gacagtcact ctactgagga cagtgaaacc tgagtttgag aagttttcca
1081tggaggtgaa aagttcagtt gagaaacaag ggctcaatat ggaggattac gttaacatgt
1141ttgttgaagg attccacgat gccatcctcc tctacgtctt ggctctacat gaagtactca
1201gagctggtta cagcaaaaag gatggaggga aaattataca gcagacttgg aacagaacat
1261ttgaaggtat cgccgggcag gtgtccatag atgccaacgg agaccgatat ggggatttct
1321ctgtgattgc catgactgat gtggaggcgg gcacccagga ggttattggt gattattttg
1381gaaaagaagg tcgttttgaa atgcggccga atgtcaaata tccttggggc cctttaaaaC
1441TGAGAATAGA TGAAAACCGA ATTGTAGAGC ATACAAACAG CTCTCCCTGC AAATCATCAG
1501GTGGCCTAGA AGAATCGGCA GTGACAGGAA TTGTCGTGGG GGCTTTACTA GGAGCTGGCT
1561TGCTAATGGC CTTCTACTTT TTCAGGAAGA AATACAGAAT AACCATTGAG AGGCGAACCC
1621AGCAAGAAGA AAGTAACCTT GGAAAACATC GGGAATTACG GGAAGATTCC ATCAGATCCC
1681ATTTTTCAGT AGCTTAAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1741TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1801GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTTGACC TCCTCCCAGT
1861ACTTTTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)