Transcript: Human NM_001363652.2

Homo sapiens natriuretic peptide receptor 3 (NPR3), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
NPR3 (4883)
Length:
6367
CDS:
15..992

Additional Resources:

NCBI RefSeq record:
NM_001363652.2
NBCI Gene record:
NPR3 (4883)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001363652.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000357661 CAATATGGAGGATTACGTTAA pLKO_005 410 CDS 100% 10.800 15.120 N NPR3 n/a
2 TRCN0000418887 GCATAGATCTCATGCAGATAG pLKO_005 4409 3UTR 100% 10.800 8.640 N NPR3 n/a
3 TRCN0000009204 CCGAATTGTAGAGCATACAAA pLKO.1 752 CDS 100% 0.563 0.450 N NPR3 n/a
4 TRCN0000357587 AGGAGGTTATTGGTGATTATT pLKO_005 652 CDS 100% 15.000 10.500 N NPR3 n/a
5 TRCN0000357659 TATCGTGTCACTCTGTTAAAT pLKO_005 1209 3UTR 100% 15.000 10.500 N NPR3 n/a
6 TRCN0000417372 TCTACAGACCCTACTATAATT pLKO_005 4124 3UTR 100% 15.000 10.500 N NPR3 n/a
7 TRCN0000009202 CCAGGAGGTTATTGGTGATTA pLKO.1 650 CDS 100% 13.200 9.240 N NPR3 n/a
8 TRCN0000421373 GAGATCAAATAGAGTATTATG pLKO_005 4515 3UTR 100% 13.200 9.240 N NPR3 n/a
9 TRCN0000418303 GGATGCAGACATGAGAGTAAA pLKO_005 4611 3UTR 100% 13.200 9.240 N NPR3 n/a
10 TRCN0000412630 GTTGGTAATGAGCTGAATTTC pLKO_005 4718 3UTR 100% 13.200 9.240 N NPR3 n/a
11 TRCN0000357660 GATGCCAACGGAGACCGATAT pLKO_005 585 CDS 100% 10.800 7.560 N NPR3 n/a
12 TRCN0000167967 CCATGTTTAATGGAGGGAAGA pLKO.1 4161 3UTR 100% 4.050 2.835 N NPR3 n/a
13 TRCN0000009201 CCTGAGATTCTTTAAGGAGAT pLKO.1 1021 3UTR 100% 4.050 2.835 N NPR3 n/a
14 TRCN0000172454 CTTCTCCCTATTCACAGGCTT pLKO.1 4259 3UTR 100% 2.640 1.848 N NPR3 n/a
15 TRCN0000168284 GTTTAATGGAGGGAAGAGAGA pLKO.1 4165 3UTR 100% 2.640 1.848 N NPR3 n/a
16 TRCN0000168660 GAAGAGAGAAATGCATGGGAA pLKO.1 4177 3UTR 100% 2.640 1.584 N NPR3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001363652.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488510 AAGGGAAACGTAGGGATGCTCTCT pLX_317 23.3% 58.6% 52.8% V5 (many diffs) n/a
2 TRCN0000489823 CTTGACCTCCTCCCAGTACTTTTG pLX_317 16.2% 58.6% 52.8% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_14719 pDONR223 52.4% 58.4% 1.9% None (many diffs) n/a
4 ccsbBroad304_14719 pLX_304 0% 58.4% 1.9% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000476668 CGTGTTGAAGCGCTGCGCAGTTTG pLX_317 27.1% 58.4% 1.9% V5 (not translated due to prior stop codon) (many diffs) n/a
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