Transcript: Mouse XM_017316493.1

PREDICTED: Mus musculus natriuretic peptide receptor 3 (Npr3), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Npr3 (18162)
Length:
6326
CDS:
197..1045

Additional Resources:

NCBI RefSeq record:
XM_017316493.1
NBCI Gene record:
Npr3 (18162)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017316493.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000120161 CATTGGTGATTACTTTGGGAA pLKO.1 712 CDS 100% 2.640 3.696 N Npr3 n/a
2 TRCN0000026212 CGGTTCCAAATGCGATCGAAT pLKO.1 740 CDS 100% 4.950 3.960 N Npr3 n/a
3 TRCN0000120158 CTCCTCTACGTTCTGGCTTTA pLKO.1 515 CDS 100% 10.800 7.560 N Npr3 n/a
4 TRCN0000120160 CAGTGGAGACTACGCTTTCTT pLKO.1 259 CDS 100% 5.625 3.938 N Npr3 n/a
5 TRCN0000026206 CCTTTGAAACTGAGACTAGAT pLKO.1 779 CDS 100% 4.950 3.465 N Npr3 n/a
6 TRCN0000120157 CTCTGAAGCTAAACAAGCATA pLKO.1 346 CDS 100% 4.950 3.465 N Npr3 n/a
7 TRCN0000026145 GCAGACTTGGAACAGGACATT pLKO.1 589 CDS 100% 4.950 3.465 N Npr3 n/a
8 TRCN0000166364 CACACACACACACACACACAA pLKO.1 4506 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017316493.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000488510 AAGGGAAACGTAGGGATGCTCTCT pLX_317 23.3% 46.7% 49.3% V5 (many diffs) n/a
2 TRCN0000489823 CTTGACCTCCTCCCAGTACTTTTG pLX_317 16.2% 46.7% 49.3% V5 (not translated due to prior stop codon) (many diffs) n/a
3 ccsbBroadEn_14719 pDONR223 52.4% 46.5% .5% None (many diffs) n/a
4 ccsbBroad304_14719 pLX_304 0% 46.5% .5% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000476668 CGTGTTGAAGCGCTGCGCAGTTTG pLX_317 27.1% 46.5% .5% V5 (not translated due to prior stop codon) (many diffs) n/a
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