Construct: ORF TRCN0000489841
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021438.1_s317c1
- DNA Barcode:
- CCTCCTTCCTTGGGTTCTATCTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PPP2CA (5515)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489841
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5515 | PPP2CA | protein phosphatase 2 catal... | NM_002715.4 | 99.3% | 100% | 927_928insTAAGCT |
| 2 | human | 5516 | PPP2CB | protein phosphatase 2 catal... | NM_001009552.2 | 82.6% | 97.4% | (many diffs) |
| 3 | human | 5515 | PPP2CA | protein phosphatase 2 catal... | NM_001355019.1 | 78.4% | 78.9% | 0_1ins195;732_733insTAAGCT |
| 4 | human | 5515 | PPP2CA | protein phosphatase 2 catal... | NR_149151.1 | 33.3% | (many diffs) | |
| 5 | mouse | 19052 | Ppp2ca | protein phosphatase 2 (form... | NM_019411.4 | 93.6% | 99.6% | (many diffs) |
| 6 | mouse | 19053 | Ppp2cb | protein phosphatase 2 (form... | NM_017374.3 | 81.3% | 97.4% | (many diffs) |
| 7 | mouse | 19053 | Ppp2cb | protein phosphatase 2 (form... | XM_006509033.1 | 63.8% | 78.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 1020
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgga tccggtggca tcatggacga gaaggtgttc accaaggagc 121 tggaccagtg gatcgagcag ctgaacgagt gcaagcagct gtccgagtcc caggtcaaga 181 gcctctgcga gaaggctaaa gaaatcctga caaaagaatc caacgtgcaa gaggttcgat 241 gtccagttac tgtctgtgga gatgtgcatg ggcaatttca tgatctcatg gaactgttta 301 gaattggtgg caaatcacca gatacaaatt acttgtttat gggagattat gttgacagag 361 gatattattc agttgaaaca gttacactgc ttgtagctct taaggttcgt taccgtgaac 421 gcatcaccat tcttcgaggg aatcatgaga gcagacagat cacacaagtt tatggtttct 481 atgatgaatg tttaagaaaa tatggaaatg caaatgtttg gaaatatttt acagatcttt 541 ttgactatct tcctctcact gccttggtgg atgggcagat cttctgtcta catggtggtc 601 tctcgCCATC TATAGATACA CTGGATCATA TCAGAGCACT TGATCGCCTA CAAGAAGTTC 661 CCCATGAGGG TCCAATGTGT GACTTGCTGT GGTCAGATCC AGATGACCGT GGTGGTTGGG 721 GTATATCTCC TCGAGGAGCT GGTTACACCT TTGGGCAAGA TATTTCTGAG ACATTTAATC 781 ATGCCAATGG CCTCACGTTG GTGTCTAGAG CTCACCAGCT AGTGATGGAG GGATATAACT 841 GGTGCCATGA CCGGAATGTA GTAACGATTT TCAGTGCTCC AAACTATTGT TATCGTTGTG 901 GTAACCAAGC TGCAATCATG GAACTTGACG ATACTCTAAA ATACTCTTTC TTGCAGTTTG 961 ACCCAGCACC TCGTAGAGGC GAGCCACATG TTACTCGTCG TACCCCAGAC TACTTCCTGT 1021 AAGCTTAAGG GTGGGCGCGC CGACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG 1081 CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT 1141 GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACCT CCTTCCTTGG 1201 GTTCTATCTT CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat 1261 t