Construct: ORF TRCN0000489889
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021947.1_s317c1
- DNA Barcode:
- TCAAGTATCAGACCACTAAACATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EGFR (1956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489889
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2369C>T | 99.9% | 99.9% | 2361G>A;2573T>G;3309C>A |
| 2 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2573T>G | 99.9% | 99.9% | 2361G>A;2369C>T;3309C>A |
| 3 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.5 | 99.8% | 99.8% | (many diffs) |
| 4 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2239_2247del | 99.6% | 99.5% | (many diffs) |
| 5 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2240_2254del | 99.4% | 99.4% | (many diffs) |
| 6 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346899.1 | 96.1% | 96% | (many diffs) |
| 7 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346900.2 | 95.4% | 95.4% | (many diffs) |
| 8 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346898.2 | 92.5% | 90.2% | (many diffs) |
| 9 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346897.2 | 88.8% | 86.4% | (many diffs) |
| 10 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346941.2 | 77.3% | 74.9% | (many diffs) |
| 11 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201284.2 | 56.2% | 50.7% | (many diffs) |
| 12 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201282.2 | 51.8% | 51.8% | 1881G>A;1883C>G;1884_1885ins1746 |
| 13 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201283.1 | 33.4% | 33.3% | 1206_1207insG;1212_1213delGAinsTT;1215_1216ins2414 |
| 14 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_207655.2 | 84.6% | 90.1% | (many diffs) |
| 15 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_007912.4 | 44.9% | 46.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3702
- ORF length:
- 3630
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcgaccc tccgggacgg ccggggcagc gctcctggcg ctgctggctg 121 cgctctgccc ggcgagtcgg gctctggagg aaaagaaagt ttgccaaggc acgagtaaca 181 agctcacgca gttgggcact tttgaagatc attttctcag cctccagagg atgttcaata 241 actgtgaggt ggtccttggg aatttggaaa ttacctatgt gcagaggaat tatgatcttt 301 ccttcttaaa gaccatccag gaggtggctg gttatgtcct cattgccctc aacacagtgg 361 agcgaattcc tttggaaaac ctgcagatca tcagaggaaa tatgtactac gaaaattcct 421 atgccttagc agtcttatct aactatgatg caaataaaac cggactgaag gagctgccca 481 tgagaaattt acaggaaatc ctgcatggcg ccgtgcggtt cagcaacaac cctgccctgt 541 gcaacgtgga gagcatccag tggcgggaca tagtcagcag tgactttctc agcaacatgt 601 cgatggactt ccagaaccac ctgggcagct gccaaaagtg tgatccaagc tgtcccaatg 661 ggagctgctg gggtgcagga gaggagaact gccagaaact gaccaaaatc atctgtgccc 721 agcagtgctc cgggcgctgc cgtggcaagt cccccagtga ctgctgccac aaccagtgtg 781 ctgcaggctg cacaggcccc cgggagagcg actgcctggt ctgccgcaaa ttccgagacg 841 aagccacgtg caaggacacc tgccccccac tcatgctcta caaccccacc acgtaccaga 901 tggatgtgaa ccccgagggc aaatacagct ttggtgccac ctgcgtgaag aagtgtcccc 961 gtaattatgt ggtgacagat cacggctcgt gcgtccgagc ctgtggggcc gacagctatg 1021 agatggagga agacggcgtc cgcaagtgta agaagtgcga agggccttgc cgcaaagtgt 1081 gtaacggaat aggtattggt gaatttaaag actcactctc cataaatgct acgaatatta 1141 aacacttcaa aaactgcacc tccatcagtg gcgatctcca catcctgccg gtggcattta 1201 ggggtgactc cttcacacat actcctcctc tggatccaca ggaactggat attctgaaaa 1261 ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac aggacggacc 1321 tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat ggtcagtttt 1381 ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc aaggagataa 1441 gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat acaataaact 1501 ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac agaggtgaaa 1561 acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag ggctgctggg 1621 gcccggagcc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg gaatgcgtgg 1681 acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct gagtgcatac 1741 agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga cggggaccag 1801 acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag acctgcccgg 1861 caggagtcat gggagaaaac aacaccctgg tctggaagta cgcagacgcc ggccatgtgt 1921 gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt gaaggctgtc 1981 caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc ctcctcttgc 2041 tgctggtggt ggccctgggg atcggcctct tcatgcgaag gcgccacatc gttcggaagc 2101 gcacgctgcg gaggctgctg caggagaggg agcttgtgga gcctcttaca cccagtggag 2161 aagctcccaa ccaagctctc ttgaggatct tgaaggaaac tgaattcaaa aagatcaaag 2221 tgctgggctc cggtgcgttc ggcacggtgt ataagggact ctggatccca gaaggtgaga 2281 aagttaaaat tcccgtcgct atcaaggaat taagagaagc aacatctccg aaagccaaca 2341 aggaaatcct cgatgaagcc tacgtgatgg ccagcgtgga caacccccac gtgtgccgcc 2401 tgctgggcat ctgcctcacc tccaccgtgc aactcatcat gcagctcatg cccttcggct 2461 gcctcctgga ctatgtccgg gaacacaaag acaatattgg ctcccagtac ctgctcaact 2521 ggtgtgtgca gatcgcaaag ggcatgaact acttggagga ccgtcgcttg gtgcaccgcg 2581 acctggcagc caggaacgta ctggtgaaaa caccgcagca tgtcaagatc acagattttg 2641 ggcgggccaa actgctgggt gcggaagaga aagaatacca tgcagaagga ggcaaagtgc 2701 ctatcaagtg gatggcattg gaatcaattt tacacagaat ctatacccac cagagtgatg 2761 tctggagcta cggggtgacc gtttgggagt tgatgacctt tggatccaag ccatatgacg 2821 gaatccctgc cagcgagatc tcctccatcc tggagaaagg agaacgcctc cctcagccac 2881 ccatatgtac catcgatgtc tacatgatca tggtcaagtg ctggatgata gacgcagata 2941 gtcgcccaaa gttccgtgag ttgatcatcg aattctccaa aatggcccga gacccccagc 3001 gctaccttgt cattcagggg gatgaaagaa tgcatttgcc aagtcctaca gactccaact 3061 tctaccgtgc cctgatggat gaagaagaca tggacgacgt ggtggatgcc gacgagtacc 3121 tcatcccaca gcagggcttc ttcagcagcc cctccacgtc acggactccc ctcctgagct 3181 ctctgagtgc aaccagcaac aattccaccg tggcttgcat tgatagaaat gggctgcaaa 3241 gctgtcccat caaggaagac agcttcttgc agcgatacag ctcagacccc acaggcgcct 3301 tGACTGAGGA CAGCATAGAC GACACCTTCC TCCCAGTGCC TGAATACATA AACCAGTCCG 3361 TTCCCAAAAG GCCCGCTGGA TCTGTGCAGA ATCCTGTCTA TCACAATCAG CCTCTGAACC 3421 CCGCGCCCAG CAGAGACCCA CACTACCAGG ACCCCCACAG CACTGCAGTG GGCAACCCCG 3481 AGTATCTCAA CACTGTCCAG CCCACCTGTG TCAACAGCAC ATTCGACAGC CCTGCCCACT 3541 GGGCCCAGAA AGGCAGCCAC CAAATTAGCC TGGACAACCC TGACTACCAG CAGGACTTCT 3601 TTCCCAAGGA AGCCAAGCCA AATGGCATCT TTAAGGGCTC CACAGCTGAA AATGCAGAAT 3661 ACCTAAGGGT CGCGCCACAA AGCAGTGAAT TTATTGGAGC ATGAGACCCA GCTTTCTTGT 3721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 3781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 3841 GAAAGGACGA TCAAGTATCA GACCACTAAA CATCACGCGT TAAGTCgaca atcaacctct 3901 ggattacaaa atttgtgaaa gatt