Construct: ORF TRCN0000489952
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020240.1_s317c1
- DNA Barcode:
- CTTCACAGTAGCCTGCTCCATTTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PMS1 (5378)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489952
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | NM_001321049.2 | 91.3% | 90% | 477_478insA;498_543del |
| 2 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | NM_001321051.1 | 87.3% | 76.7% | (many diffs) |
| 3 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | NM_001321044.2 | 21.7% | 20.1% | (many diffs) |
| 4 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | NM_001128144.2 | 20.6% | 19% | (many diffs) |
| 5 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | XM_017004348.1 | 20.6% | 19% | (many diffs) |
| 6 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | NM_001128143.2 | 17.8% | 16.4% | (many diffs) |
| 7 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | XM_017004344.1 | 17.8% | 16.4% | (many diffs) |
| 8 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | XM_024452966.1 | 17.8% | 16.4% | (many diffs) |
| 9 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | XM_024452967.1 | 17.8% | 16.4% | (many diffs) |
| 10 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | NM_000534.4 | 17% | 15.7% | (many diffs) |
| 11 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | NM_001321045.2 | 17% | 15.7% | (many diffs) |
| 12 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | NM_001321047.2 | 17% | 15.7% | (many diffs) |
| 13 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | NM_001321048.2 | 17% | 15.7% | (many diffs) |
| 14 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | XM_024452964.1 | 17% | 15.7% | (many diffs) |
| 15 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | XM_024452965.1 | 17% | 15.7% | (many diffs) |
| 16 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | NR_110332.2 | 16.5% | (many diffs) | |
| 17 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | XR_922951.2 | 15.2% | (many diffs) | |
| 18 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | XR_001738779.1 | 14.8% | (many diffs) | |
| 19 | human | 5378 | PMS1 | PMS1 homolog 1, mismatch re... | XR_002959307.1 | 14.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 570
- ORF length:
- 498
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaaacaa ttgcctgcgg caacagttcg actcctttca agttctcaga 121 tcatcacttc ggtggtcagt gttgtaaaag agcttattGA AAACTCCTTG GATGCTGGTG 181 CCACAAGCGT AGATGTTAAA CTGGAGAACT ATGGATTTGA TAAAATTGAG GTGCGAGATA 241 ACGGGGAGGG TATCAAGGCT GTTGATGCAC CTGTAATGGC AATGAAGTAC TACACCTCAA 301 AAATAAATAG TCATGAAGAT CTTGAAAATT TGACAACTTA CGGTTTTCGT GGAGAAGCCT 361 TGGGGTCAAT TTGTTGTATA GCTGAGGTTT TAATTACAAC AAGAACGGCT GCTGATAATT 421 TTAGCACCCA GTATGTTTTA GATGGCAGTG GCCACATACT TTCTCAGAAA CCTTCACATC 481 TTGGTCAAGG TAAGAAAGTA GCTTTGTATA CAAACATTCT TTACCTTTTC TGTCTTAATT 541 GTTGGTTTAA AAAAAAAAAA GTTACTCGGT GAGACCCAGC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACT 721 TCACAGTAGC CTGCTCCATT TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt