Construct: ORF TRCN0000491325
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020473.2_s317c1
- DNA Barcode:
- AAGCGTCCACGACTTTACTTCTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- LATS2 (26524)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491325
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26524 | LATS2 | large tumor suppressor kina... | NM_014572.3 | 94.3% | 94.3% | (many diffs) |
| 2 | human | 26524 | LATS2 | large tumor suppressor kina... | XM_005266342.1 | 94.3% | 94.3% | (many diffs) |
| 3 | human | 26524 | LATS2 | large tumor suppressor kina... | XM_011535042.2 | 85.4% | 84.1% | (many diffs) |
| 4 | human | 26524 | LATS2 | large tumor suppressor kina... | XM_017020541.1 | 84.3% | 83.8% | (many diffs) |
| 5 | human | 26524 | LATS2 | large tumor suppressor kina... | XM_017020542.1 | 82.2% | 78.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3153
- ORF length:
- 3081
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaggcca aagacttttc ctgccacgac ttattctgga aatagccggc 121 agcgactgca agagattcgt gaggggttaa agcagccatc caagtcttcg gttcaggggc 181 tacccgcagg accaaacagt gacacttccc tggatgccaa agtcctgggg agcaaagatg 241 ccaccaggca gcagcagcag atgagagcca ccccaaagtt cggaccttat cagaaagcct 301 tgagggaaat cagatattcc ttgttgcctt ttgctaatga atcgggcacc tctgcagctg 361 cagaagtgaa ccggcaaatg ctgcaggaac tggtgaacgc aggatgcgac caggagatgg 421 ctggccgagc tctcaagcag actggcagca ggagcatcga ggccgccctg gagtacatca 481 gcaagatggg ctacctggac ccgaggaatg agcagattgt gcgggtcatt aagcagacct 541 ccccaggaaa ggggctcatg ccaaccccag tgacgcggag gcccagcttc gaaggaaccg 601 gcgattcgtt tgcgtcctac caccagctga gcggtacccc ctacgagggc ccaagcttcg 661 gcgctgacgg ccccacggcg ctggaggaga tgccgcggcc gtacgtggac taccttttcc 721 ccggagtcgg cccccacggg cccggccacc agcaccagca cccacccaag ggctacggtg 781 ccagcgtaga ggcagcaggg gcacacttcc cgctgcaggg cgcgcactac gggcggccgc 841 acctgctggt gcctggggaa cccctgggct acggagtgca gcgcagcccc tccttccaga 901 gcaagacgcc gccggagacc gggggttacg ccagcctgcc cacgaagggc cagggaggac 961 cgccaggcgc cggcctcgct ttcccacccc ctgccgccgg gctctacgtg ccgcacccac 1021 accacaagca ggccggtccc gcggcccacc agctgcatgt gctgggctcc cgcagccagg 1081 tgttcgccag cgacagcccc ccgcagagcc tgctcactcc ctcgcggaac agcctcaacg 1141 tggacctgta tgaattgagc agcacctccg tccagcagtg gccggctgcc accctggccc 1201 gccgggactc cctgcagaag ccgggcctgg aggcgccgcc gcgcgcgcac gtggccttcc 1261 ggcctgactg cccagtgccc agcaggacca actccttcaa cagccaccag ccgcggcccg 1321 gtccgcctgg caaggccgag ccctccctgc ccgcccccaa caccgtgacg gctgtcacgg 1381 ccgcgcacat cttgcacccg gtgaagagcg tgcgtgtgct gaggccggag ccgcagacgg 1441 ctgtggggcc ctcgcacccc gcctgggtgc ccgcgcctgc cccggccccc gcccccgccc 1501 ccgccccggc tgcggagggc ttggacgcca aggaggagca tgccctggcg ctgggcggcg 1561 caggcgcctt cccgctggac gtggagtacg gaggcccaga ccggaggtgc ccgcctccac 1621 cctacccgaa gcacctgctg ctgcgcagca agtcggagca gtacgacctg gacagcctgt 1681 gcgcaggcat ggagcagagc ctccgtgcgg gccccaacga gcccgagggc ggcgacaaga 1741 gccgcaaaag cgccaagggg gacaaaggcg gaaaggataa aaagcagatt cagacctctc 1801 ccgttcccgt ccgcaaaaac agcagagacg aagagaagag agagtcacgc atcaagagct 1861 actcgccata cgcctttaag ttcttcatgg agcagcacgt ggagaatgtc atcaaaacct 1921 accagcagaa ggttaaccgg aggctgcagc tggagcaaga aatggccaaa gctggactct 1981 gtgaagctga gcaggagcag atgcggaaga tcctctacca gaaagagtct aattacaaca 2041 ggttaaagag ggccaagatg gacaagtcta tgtttgtcaa gatcaaaacc ctggggatcg 2101 gtgcctttgg agaagtgtgc cttgcttgta aggtggacac tcacgccctg tacgccatga 2161 agaccctaag gaaaaaggat gtcctgaacc ggaatcaggt ggcccacgtc aaggccgaga 2221 gggacatcct ggccgaggca gacaatgagt gggtggtcaa actctactac tccttccaag 2281 acaaagacag cctgtacttt gtgatggact acatccctgg tggggacatg atgagcctgc 2341 tgatccggat ggaggtcttc cctgagcacc tggcccggtt ctacatcgca gagctgactt 2401 tggccattga gagtgtccac aagatgggct tcatccaccg agacatcaag cctgataaca 2461 ttttgataga tctggatggt cacattaaac tcacagattt cggcctctgc actgggttca 2521 ggtggactca caattccaaa tattaccaga aagggtacac tcaactctgt gactggtgga 2581 gtgttggagt gattctcttc gagatgctgg tggggcagcc gccctttttg gcacctactc 2641 ccacagaaac ccagctgaag gtgatcaact gggagaacac gctccacatt ccagcccagg 2701 tgaagctgag ccctgaggcc agggacctca tcaccaagct gtgctgctcc gcagaccacc 2761 gcctggggcg gaatggggcc gatgacctga aggcccaccc cttcttcagc gccattgact 2821 tctccagtga catccggaag cagccagccc cctacgttcc caccatcagc caccccatgg 2881 acacctcgaa TTTCGACCCC GTAGATGAAG AAAGCCCTTG GAACGATGCC AGCGAAGGTA 2941 GCACCAAGGC CTGGGACACA CTCACCTCGC CCAATAACAA GCATCCTGAG CACGCATTTT 3001 ACGAATTCAC CTTCCGAAGG TTCTTTGATG ACAATGGCTA CCCCTTTCGA TGCCCAAAGC 3061 CTTCAGGAGC AGAAGCTTCA CAGGCTGAGA GCTCAGATTT AGAAAGCTCT GATCTGGTGG 3121 ATCAGACTGA AGGCTGCCAG CCTGTGTACG TGTAGGACCC AGCTTTCTTG TACAAAGTGG 3181 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 3241 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 3301 AAAGCGTCCA CGACTTTACT TCTCCACGCG TTAAGTCgac aatcaacctc tggattacaa 3361 aatttgtgaa agatt