Construct: ORF TRCN0000491325

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020473.2_s317c1
DNA Barcode:
AAGCGTCCACGACTTTACTTCTCC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
LATS2 (26524)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491325
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26524 LATS2 large tumor suppressor kina... NM_014572.3 94.3% 94.3% (many diffs)
2 human 26524 LATS2 large tumor suppressor kina... XM_005266342.1 94.3% 94.3% (many diffs)
3 human 26524 LATS2 large tumor suppressor kina... XM_011535042.2 85.4% 84.1% (many diffs)
4 human 26524 LATS2 large tumor suppressor kina... XM_017020541.1 84.3% 83.8% (many diffs)
5 human 26524 LATS2 large tumor suppressor kina... XM_017020542.1 82.2% 78.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3153
ORF length:
3081
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaggcca aagacttttc ctgccacgac ttattctgga aatagccggc
121agcgactgca agagattcgt gaggggttaa agcagccatc caagtcttcg gttcaggggc
181tacccgcagg accaaacagt gacacttccc tggatgccaa agtcctgggg agcaaagatg
241ccaccaggca gcagcagcag atgagagcca ccccaaagtt cggaccttat cagaaagcct
301tgagggaaat cagatattcc ttgttgcctt ttgctaatga atcgggcacc tctgcagctg
361cagaagtgaa ccggcaaatg ctgcaggaac tggtgaacgc aggatgcgac caggagatgg
421ctggccgagc tctcaagcag actggcagca ggagcatcga ggccgccctg gagtacatca
481gcaagatggg ctacctggac ccgaggaatg agcagattgt gcgggtcatt aagcagacct
541ccccaggaaa ggggctcatg ccaaccccag tgacgcggag gcccagcttc gaaggaaccg
601gcgattcgtt tgcgtcctac caccagctga gcggtacccc ctacgagggc ccaagcttcg
661gcgctgacgg ccccacggcg ctggaggaga tgccgcggcc gtacgtggac taccttttcc
721ccggagtcgg cccccacggg cccggccacc agcaccagca cccacccaag ggctacggtg
781ccagcgtaga ggcagcaggg gcacacttcc cgctgcaggg cgcgcactac gggcggccgc
841acctgctggt gcctggggaa cccctgggct acggagtgca gcgcagcccc tccttccaga
901gcaagacgcc gccggagacc gggggttacg ccagcctgcc cacgaagggc cagggaggac
961cgccaggcgc cggcctcgct ttcccacccc ctgccgccgg gctctacgtg ccgcacccac
1021accacaagca ggccggtccc gcggcccacc agctgcatgt gctgggctcc cgcagccagg
1081tgttcgccag cgacagcccc ccgcagagcc tgctcactcc ctcgcggaac agcctcaacg
1141tggacctgta tgaattgagc agcacctccg tccagcagtg gccggctgcc accctggccc
1201gccgggactc cctgcagaag ccgggcctgg aggcgccgcc gcgcgcgcac gtggccttcc
1261ggcctgactg cccagtgccc agcaggacca actccttcaa cagccaccag ccgcggcccg
1321gtccgcctgg caaggccgag ccctccctgc ccgcccccaa caccgtgacg gctgtcacgg
1381ccgcgcacat cttgcacccg gtgaagagcg tgcgtgtgct gaggccggag ccgcagacgg
1441ctgtggggcc ctcgcacccc gcctgggtgc ccgcgcctgc cccggccccc gcccccgccc
1501ccgccccggc tgcggagggc ttggacgcca aggaggagca tgccctggcg ctgggcggcg
1561caggcgcctt cccgctggac gtggagtacg gaggcccaga ccggaggtgc ccgcctccac
1621cctacccgaa gcacctgctg ctgcgcagca agtcggagca gtacgacctg gacagcctgt
1681gcgcaggcat ggagcagagc ctccgtgcgg gccccaacga gcccgagggc ggcgacaaga
1741gccgcaaaag cgccaagggg gacaaaggcg gaaaggataa aaagcagatt cagacctctc
1801ccgttcccgt ccgcaaaaac agcagagacg aagagaagag agagtcacgc atcaagagct
1861actcgccata cgcctttaag ttcttcatgg agcagcacgt ggagaatgtc atcaaaacct
1921accagcagaa ggttaaccgg aggctgcagc tggagcaaga aatggccaaa gctggactct
1981gtgaagctga gcaggagcag atgcggaaga tcctctacca gaaagagtct aattacaaca
2041ggttaaagag ggccaagatg gacaagtcta tgtttgtcaa gatcaaaacc ctggggatcg
2101gtgcctttgg agaagtgtgc cttgcttgta aggtggacac tcacgccctg tacgccatga
2161agaccctaag gaaaaaggat gtcctgaacc ggaatcaggt ggcccacgtc aaggccgaga
2221gggacatcct ggccgaggca gacaatgagt gggtggtcaa actctactac tccttccaag
2281acaaagacag cctgtacttt gtgatggact acatccctgg tggggacatg atgagcctgc
2341tgatccggat ggaggtcttc cctgagcacc tggcccggtt ctacatcgca gagctgactt
2401tggccattga gagtgtccac aagatgggct tcatccaccg agacatcaag cctgataaca
2461ttttgataga tctggatggt cacattaaac tcacagattt cggcctctgc actgggttca
2521ggtggactca caattccaaa tattaccaga aagggtacac tcaactctgt gactggtgga
2581gtgttggagt gattctcttc gagatgctgg tggggcagcc gccctttttg gcacctactc
2641ccacagaaac ccagctgaag gtgatcaact gggagaacac gctccacatt ccagcccagg
2701tgaagctgag ccctgaggcc agggacctca tcaccaagct gtgctgctcc gcagaccacc
2761gcctggggcg gaatggggcc gatgacctga aggcccaccc cttcttcagc gccattgact
2821tctccagtga catccggaag cagccagccc cctacgttcc caccatcagc caccccatgg
2881acacctcgaa TTTCGACCCC GTAGATGAAG AAAGCCCTTG GAACGATGCC AGCGAAGGTA
2941GCACCAAGGC CTGGGACACA CTCACCTCGC CCAATAACAA GCATCCTGAG CACGCATTTT
3001ACGAATTCAC CTTCCGAAGG TTCTTTGATG ACAATGGCTA CCCCTTTCGA TGCCCAAAGC
3061CTTCAGGAGC AGAAGCTTCA CAGGCTGAGA GCTCAGATTT AGAAAGCTCT GATCTGGTGG
3121ATCAGACTGA AGGCTGCCAG CCTGTGTACG TGTAGGACCC AGCTTTCTTG TACAAAGTGG
3181TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
3241CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
3301AAAGCGTCCA CGACTTTACT TCTCCACGCG TTAAGTCgac aatcaacctc tggattacaa
3361aatttgtgaa agatt

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