Construct: ORF TRCN0000491407
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017745.1_s317c1
- Derived from:
- ccsbBroadEn_01428
- DNA Barcode:
- ATACTTCTGGCTACTGGTCGTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL26 (6154)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491407
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6154 | RPL26 | ribosomal protein L26 | NM_000987.5 | 100% | 100% | |
| 2 | human | 6154 | RPL26 | ribosomal protein L26 | NM_001315530.2 | 100% | 100% | |
| 3 | human | 6154 | RPL26 | ribosomal protein L26 | NM_001315531.2 | 100% | 100% | |
| 4 | human | 51121 | RPL26L1 | ribosomal protein L26 like 1 | NM_001317980.1 | 84.1% | 98.6% | (many diffs) |
| 5 | human | 51121 | RPL26L1 | ribosomal protein L26 like 1 | NM_001317981.1 | 84.1% | 98.6% | (many diffs) |
| 6 | human | 51121 | RPL26L1 | ribosomal protein L26 like 1 | NM_001317982.1 | 84.1% | 98.6% | (many diffs) |
| 7 | human | 51121 | RPL26L1 | ribosomal protein L26 like 1 | NM_016093.3 | 84.1% | 98.6% | (many diffs) |
| 8 | human | 51121 | RPL26L1 | ribosomal protein L26 like 1 | XM_011534565.2 | 84.1% | 98.6% | (many diffs) |
| 9 | human | 51121 | RPL26L1 | ribosomal protein L26 like 1 | XM_017009519.2 | 84.1% | 98.6% | (many diffs) |
| 10 | human | 51121 | RPL26L1 | ribosomal protein L26 like 1 | XM_017009520.1 | 84.1% | 98.6% | (many diffs) |
| 11 | mouse | 19941 | Rpl26 | ribosomal protein L26 | NM_009080.2 | 87.8% | 100% | (many diffs) |
| 12 | mouse | 100034726 | Gm15772 | predicted gene 15772; ribos... | NR_003373.1 | 71.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 504
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagtttaat ccctttgtga cttccgaccg aagcaagaat cgcaaaaggc 121 atttcaatgc accttcccac attcgaagga agattatgTC TTCCCCTCTT TCCAAAGAGC 181 TGAGACAGAA GTACAACGTG CGATCCATGC CCATCCGAAA GGATGATGAA GTTCAGGTTG 241 TACGTGGACA CTATAAAGGT CAGCAAATTG GCAAAGTAGT CCAGGTTTAC AGGAAGAAAT 301 ATGTTATCTA CATTGAACGG GTGCAGCGGG AAAAGGCTAA TGGCACAACT GTCCACGTAG 361 GCATTCACCC CAGCAAGGTG GTTATCACTA GGCTAAAACT GGACAAAGAC CGCAAAAAGA 421 TCCTCGAACG GAAAGCCAAA TCTCGCCAAG TAGGAAAGGA AAAGGGCAAA TACAAGGAAG 481 AAACCATTGA GAAGATGCAG GAATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 541 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 601 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA TACTTCTGGC 661 TACTGGTCGT CCAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 721 att