Construct: ORF TRCN0000491471
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019676.2_s317c1
- DNA Barcode:
- AAACGAACCACGTCGCTTGAGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHKG1 (5260)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491471
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | NM_006213.4 | 99.9% | 99.7% | 1161_1162insG |
| 2 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | NM_001258460.1 | 97.5% | 97.4% | 238_239ins27;1134_1135insG |
| 3 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | NM_001258459.1 | 92.2% | 92.1% | 318_413del;1257_1258insG |
| 4 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | XM_017012324.2 | 89.5% | 89.3% | 546_680del;1296_1297insG |
| 5 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | XM_005271772.5 | 79.7% | 79.6% | 83_84ins234;927_928insG |
| 6 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | XM_017012325.2 | 77% | 66% | (many diffs) |
| 7 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | XM_017012326.2 | 71.4% | 71.3% | 83_84ins234;312_446del;1062_1063insG |
| 8 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | XM_017012327.2 | 64.9% | 64.8% | 0_1ins318;228_362del;978_979insG |
| 9 | human | 5260 | PHKG1 | phosphorylase kinase cataly... | NR_047689.1 | 51.9% | 1_195del;457_458ins55;1302_2075delinsG | |
| 10 | mouse | 18682 | Phkg1 | phosphorylase kinase gamma 1 | NM_011079.3 | 87.1% | 93% | (many diffs) |
| 11 | mouse | 18682 | Phkg1 | phosphorylase kinase gamma 1 | XM_011240871.2 | 63.2% | 67.8% | (many diffs) |
| 12 | mouse | 18682 | Phkg1 | phosphorylase kinase gamma 1 | NR_145486.1 | 34.4% | (many diffs) | |
| 13 | mouse | 18682 | Phkg1 | phosphorylase kinase gamma 1 | NR_145487.1 | 34.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 75
- ORF end:
- 1239
- ORF length:
- 1164
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcgc caccatgacc cgggacgagg cactgccgga ctctcattct gcacaggact 121 tctatgagaa ttatgagccc aaagagatcc tgggcagggg cgttagcagt gtggtcaggc 181 gatgcatcca caagcccacg agccaggagt acgccgtgaa ggtcatcgac gtcaccggtg 241 gaggcagctt cagcccggag gaggtgcggg agctgcgaga agccacgctg aaggaggtgg 301 acatcctgcg caaggtctca gggcacccca acatcataca gctgaaggac acttatgaga 361 ccaacacttt cttcttcttg gtgtttgacc tgatgaagag aggggagctc tttgactacc 421 tcactgagaa ggtcaccttg agtgagaagg aaaccagaaa gatcatgcga gctctgctgg 481 aggtgatctg caccttgcac aaactcaaca tcgtgcaccg ggacctgaag cccgagaaca 541 ttctcttgga tgacaacatg aacatcaagc tcacagactt tggcttttcc tgccagctgg 601 agccgggaga gaggctgcga gaggtctgcg ggacccccag ttacctggcc cctgagatta 661 tcgagtgctc catgaatgag gaccacccgg gctacgggaa agaggtggac atgtggagca 721 ctggcgtcat catgtacacg ctgctggccg gctccccgcc cttctggcac cggaagcaga 781 tgctgatgct gaggatgatc atgagcggca actaccagtt tggctcgccc gagtgggatg 841 attactcgga caccgtgaag gacctggtct cccgattcct ggtggtgcaa ccccagaacc 901 gctacacagc ggaagaggcc ttggcacacc ccttcttcca gcagtacttg gtggaggaag 961 tgcggcactt cagcccccgg gggaagttca aggtgatcgc tctgaccgtg ctggcTTCAG 1021 TGCGGATCTA CTACCAGTAC CGCCGGGTGA AGCCTGTGAC CCGGGAGATC GTCATCCGAG 1081 ACCCCTATGC CCTCCGGCCT CTGCGCCGGC TCATCGACGC CTACGCTTTC CGAATCTATG 1141 GCCACTGGGT GAAGAAGGGG CAGCAGCAGA ACCGGGCAGC CCTTTTCGAG AACACACCCA 1201 AGGCCGTGCT CCTCTCCCTG GCCGAGGAGG ACTACGACCC AGCTTTCTTG TACAAAGTGG 1261 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1321 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1381 AAAACGAACC ACGTCGCTTG AGTTCACGCG TTAAGTCgac aatcaacctc tggattacaa 1441 aatttgtgaa agatt