Construct: ORF TRCN0000491475
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020622.2_s317c1
- DNA Barcode:
- TCTACTAACGTTGCATTAGTGGAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PDGFRA (5156)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491475
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347829.2 | 99.9% | 100% | 1701A>G;3222T>C |
| 2 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_006206.6 | 99.9% | 100% | 1701A>G;3222T>C |
| 3 | human | 5156 | PDGFRA | platelet derived growth fac... | XM_005265743.1 | 99.9% | 100% | 1701A>G;3222T>C |
| 4 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_006206.4:c.2522_2527del | 99.7% | 99.8% | 1701A>G;2519_2520insCAGAGA;3216T>C |
| 5 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_006206.4:c.2526_2537del | 99.5% | 99.6% | 1701A>G;2523_2524insGACATCATGCAT;3210T>C |
| 6 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347830.1 | 98.7% | 98.8% | 1_39del;1740A>G;3261T>C |
| 7 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347828.2 | 97.6% | 97.7% | 1_75del;1776A>G;3297T>C |
| 8 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347827.2 | 73.2% | 70.9% | (many diffs) |
| 9 | human | 5156 | PDGFRA | platelet derived growth fac... | XM_017008281.1 | 72.3% | 70.1% | (many diffs) |
| 10 | human | 5156 | PDGFRA | platelet derived growth fac... | XM_006714041.3 | 71.5% | 69.3% | (many diffs) |
| 11 | mouse | 18595 | Pdgfra | platelet derived growth fac... | NM_001083316.2 | 86.4% | 91.8% | (many diffs) |
| 12 | mouse | 18595 | Pdgfra | platelet derived growth fac... | NM_011058.3 | 86.4% | 91.8% | (many diffs) |
| 13 | mouse | 18595 | Pdgfra | platelet derived growth fac... | XM_006504261.3 | 86.4% | 91.8% | (many diffs) |
| 14 | mouse | 18595 | Pdgfra | platelet derived growth fac... | XM_006504262.3 | 86.4% | 91.8% | (many diffs) |
| 15 | mouse | 18595 | Pdgfra | platelet derived growth fac... | XM_006504263.3 | 86.4% | 91.8% | (many diffs) |
| 16 | mouse | 18595 | Pdgfra | platelet derived growth fac... | NM_001347718.1 | 61% | 63.2% | (many diffs) |
| 17 | mouse | 18595 | Pdgfra | platelet derived growth fac... | NM_001347719.1 | 61% | 63.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3339
- ORF length:
- 3267
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggggact tcccatccgg cgttcctggt cttaggctgt cttctcacag 121 ggctgagcct aatcctctgc cagctttcat taccctctat ccttccaaat gaaaatgaaa 181 aggttgtgca gctgaattca tccttttctc tgagatgctt tggggagagt gaagtgagct 241 ggcagtaccc catgtctgaa gaagagagct ccgatgtgga aatcagaaat gaagaaaaca 301 acagcggcct ttttgtgacg gtcttggaag tgagcagtgc ctcggcggcc cacacagggt 361 tgtacacttg ctattacaac cacactcaga cagaagagaa tgagcttgaa ggcaggcaca 421 tttacatcta tgtgccagac ccagatgtag cctttgtacc tctaggaatg acggattatt 481 tagtcatcgt ggaggatgat gattctgcca ttataccttg tcgcacaact gatcccgaga 541 ctcctgtaac cttacacaac agtgaggggg tggtacctgc ctcctacgac agcagacagg 601 gctttaatgg gaccttcact gtagggccct atatctgtga ggccaccgtc aaaggaaaga 661 agttccagac catcccattt aatgtttatg ctttaaaagc aacatcagag ctggatctag 721 aaatggaagc tcttaaaacc gtgtataagt caggggaaac gattgtggtc acctgtgctg 781 tttttaacaa tgaggtggtt gaccttcaat ggacttaccc tggagaagtg aaaggcaaag 841 gcatcacaat gctggaagaa atcaaagtcc catccatcaa attggtgtac actttgacgg 901 tccccgaggc cacggtgaaa gacagtggag attacgaatg tgctgcccgc caggctacca 961 gggaggtcaa agaaatgaag aaagtcacta tttctgtcca tgagaaaggt ttcattgaaa 1021 tcaaacccac cttcagccag ttggaagctg tcaacctgca tgaagtcaaa cattttgttg 1081 tagaggtgcg ggcctaccca cctcccagga tatcctggct gaaaaacaat ctgactctga 1141 ttgaaaatct cactgagatc accactgatg tggaaaagat tcaggaaata aggtatcgaa 1201 gcaaattaaa gctgatccgt gctaaggaag aagacagtgg ccattatact attgtagctc 1261 aaaatgaaga tgctgtgaag agctatactt ttgaactgtt aactcaagtt ccttcatcca 1321 ttctggactt ggtcgatgat caccatggct caactggggg acagacggtg aggtgcacag 1381 ctgaaggcac gccgcttcct gatattgagt ggatgatatg caaagatatt aagaaatgta 1441 ataatgaaac ttcctggact attttggcca acaatgtctc aaacatcatc acggagatcc 1501 actcccgaga caggagtacc gtggagggcc gtgtgacttt cgccaaagtg gaggagacca 1561 tcgccgtgcg atgcctggct aagaatctcc ttggagctga gaaccgagag ctgaagctgg 1621 tggctcccac cctgcgttct gaactcacgg tggctgctgc agtcctggtg ctgttggtga 1681 ttgtgatcat ctcacttatt gtcctggttg tcatttggaa acagaaaccg aggtatgaaa 1741 ttcgctggag ggtcattgaa tcaatcagcc cggatggaca tgaatatatt tatgtggacc 1801 cgatgcagct gccttatgac tcaagatggg agtttccaag agatggacta gtgcttggtc 1861 gggtcttggg gtctggagcg tttgggaagg tggttgaagg aacagcctat ggattaagcc 1921 ggtcccaacc tgtcatgaaa gttgcagtga agatgctaaa acccacggcc agatccagtg 1981 aaaaacaagc tctcatgtct gaactgaaga taatgactca cctggggcca catttgaaca 2041 ttgtaaactt gctgggagcc tgcaccaagt caggccccat ttacatcatc acagagtatt 2101 gcttctatgg agatttggtc aactatttgc ataagaatag ggatagcttc ctgagccacc 2161 acccagagaa gccaaagaaa gagctggata tctttggatt gaaccctgct gatgaaagca 2221 cacggagcta tgttatttta tcttttgaaa acaatggtga ctacatggac atgaagcagg 2281 ctgatactac acagtatgtc cccatgctag aaaggaaaga ggtttctaaa tattccgaca 2341 tccagagatc actctatgat cgtccagcct catataagaa gaaatctatg ttagactcag 2401 aagtcaaaaa cctcctttca gatgataact cagaaggcct tactttattg gatttgttga 2461 gcttcaccta tcaagttgcc cgaggaatgg agtttttggc ttcaaaaaat tgtgtccacc 2521 gtgatctggc tgctcgcaac gtcctcctgg cacaaggaaa aattgtgaag atctgtgact 2581 ttggcctggc cagagacatc atgcatgatt cgaactatgt gtcgaaaggc agtacctttc 2641 tgcccgtgaa gtggatggct cctgagagca tctttgacaa cctctacacc acactgagtg 2701 atgtctggtc ttatggcatt ctgctctggg agatcttttc ccttggtggc accccttacc 2761 ccggcatgat ggtggattct actttctaca ataagatcaa gagtgggtac cggatggcca 2821 agcctgacca cgctaccagt gaagtctacg agatcatggt gaaatgctgg aacagtgagc 2881 cggagaagag accctccttt taccacctga gtgagattgt ggagaatctg ctgcctggac 2941 aatataaaaa gagttatgaa aaaattcacc tggacttcct gaagagtgac catcctgctg 3001 tggcacgcat gcGTGTGGAC TCAGACAATG CATACATTGG TGTCACCTAC AAAAACGAGG 3061 AAGACAAGCT GAAGGACTGG GAGGGTGGTC TGGATGAGCA GAGACTGAGC GCTGACAGTG 3121 GCTACATCAT TCCTCTGCCT GACATTGACC CTGTCCCTGA GGAGGAGGAC CTGGGCAAGA 3181 GGAACAGACA CAGCTCGCAG ACCTCTGAAG AGAGTGCCAT TGAGACGGGT TCCAGCAGTT 3241 CCACCTTCAT CAAGAGAGAG GACGAGACCA TTGAAGACAT CGACATGATG GACGACATCG 3301 GCATAGACTC TTCAGACCTG GTGGAAGACA GCTTCCTGTA GAACCCAGCT TTCTTGTACA 3361 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 3421 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3481 AGGACGATCT ACTAACGTTG CATTAGTGGA TACGCGTTAA GTCgacaatc aacctctgga 3541 ttacaaaatt tgtgaaagat t