Construct: ORF TRCN0000491512
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021550.2_s317c1
- DNA Barcode:
- CGTTTACCCCCCGCCGCCCACGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RORB (6096)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491512
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6096 | RORB | RAR related orphan receptor B | NM_006914.4 | 100% | 100% | |
| 2 | human | 6096 | RORB | RAR related orphan receptor B | NM_001365023.1 | 97.5% | 97.2% | (many diffs) |
| 3 | mouse | 225998 | Rorb | RAR-related orphan receptor... | NM_001043354.2 | 92.9% | 98.2% | (many diffs) |
| 4 | mouse | 225998 | Rorb | RAR-related orphan receptor... | NM_146095.4 | 90.7% | 95.5% | (many diffs) |
| 5 | mouse | 225998 | Rorb | RAR-related orphan receptor... | NM_001289921.1 | 77.4% | 82.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1449
- ORF length:
- 1377
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcgagca caaattgaag tgataccatg caaaatttgt ggcgataagt 121 cctctgggat ccactacgga gtcatcacat gtgaaggctg caagggattc tttaggagga 181 gccagcagaa caatgcttct tattcctgcc caaggcagag aaactgttta attgacagaa 241 cgaacagaaa ccgttgccaa cactgccgac tgcagaagtg tcttgcccta ggaatgtcaa 301 gagatgctgt gaagtttggg aggatgtcca agaagcaaag ggacagcctg tatgctgagg 361 tgcagaagca ccagcagcgg ctgcaggaac agcggcagca gcagagtggg gaggcagaag 421 cccttgccag ggtgtacagc agcagcatta gcaacggcct gagcaacctg aacaacgaga 481 ccagcggcac ttatgccaac gggcacgtca ttgacctgcc caagtctgag ggttattaca 541 acgtcgattc cggtcagccg tcccctgatc agtcaggact tgacatgact ggaatcaaac 601 agataaagca agaacctatc tatgacctca catccgtacc caacttgttt acctatagct 661 ctttcaacaa tgggcagtta gcaccaggga taaccatgac tgaaatcgac cgaattgcac 721 agaacatcat taagtcccat ttggagacat gtcaatacac catggaagag ctgcaccagc 781 tggcgtggca gacccacacc tatgaagaaa ttaaagcata tcaaagcaag tccagggaag 841 cactgtggca acaatgtgcc atccagatca ctcacgccat ccaatacgtg gtggagtttg 901 caaagcggat aacaggcttc atggagctct gtcaaaatga tcaaattcta cttctgaagt 961 caggttgctt ggaagtggtt ttagtgagaa tgtgccgtgc cttcaaccca ttaaacaaca 1021 ctgttctgtt tgaaggaaaa tatggaggaa tgcaaatgtt caaagcctta ggttctgatg 1081 acctagtgaa tgaagcattt gactttgcaa agaatttgtg ttccttgcag ctgaccgagg 1141 aggagatcgc tttgttctca tctgctgttc TGATATCTCC AGACCGAGCC TGGCTTATAG 1201 AACCAAGGAA AGTCCAGAAG CTTCAGGAAA AAATTTATTT TGCACTTCAA CATGTGATTC 1261 AGAAGAATCA CCTGGATGAT GAGACCTTGG CAAAGTTAAT AGCCAAGATA CCAACCATCA 1321 CGGCAGTTTG CAACTTGCAC GGGGAGAAGC TGCAGGTATT TAAGCAATCT CATCCAGAGA 1381 TAGTGAATAC ACTGTTTCCT CCGTTATACA AGGAGCTCTT TAATCCTGAC TGTGCCACCG 1441 GCTGCAAATA GAACCCAGCT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1501 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1561 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGACGT TTACCCCCCG CCGCCCACGT 1621 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t