Construct: ORF TRCN0000491537
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021773.2_s317c1
- DNA Barcode:
- ATGCTAGCTATTGCAATTGCTAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ANXA2 (302)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491537
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 302 | ANXA2 | annexin A2 | NM_001002858.3 | 99.9% | 100% | 24T>C |
| 2 | human | 302 | ANXA2 | annexin A2 | NM_001002857.2 | 94.9% | 94.9% | 0_1ins54 |
| 3 | human | 302 | ANXA2 | annexin A2 | NM_001136015.3 | 94.9% | 94.9% | 0_1ins54 |
| 4 | human | 302 | ANXA2 | annexin A2 | NM_004039.3 | 94.9% | 94.9% | 0_1ins54 |
| 5 | human | 302 | ANXA2 | annexin A2 | XM_017022090.1 | 94.9% | 94.9% | 0_1ins54 |
| 6 | human | 302 | ANXA2 | annexin A2 | XM_017022091.1 | 94.9% | 94.9% | 0_1ins54 |
| 7 | human | 304 | ANXA2P2 | annexin A2 pseudogene 2 | NR_003573.1 | 78.2% | (many diffs) | |
| 8 | human | 303 | ANXA2P1 | annexin A2 pseudogene 1 | NR_001562.2 | 70.5% | (many diffs) | |
| 9 | human | 305 | ANXA2P3 | annexin A2 pseudogene 3 | NR_001446.2 | 70% | (many diffs) | |
| 10 | human | 302 | ANXA2 | annexin A2 | XM_017022092.1 | 62.1% | 62.1% | 0_1ins405 |
| 11 | human | 302 | ANXA2 | annexin A2 | XM_017022093.1 | 62.1% | 62.1% | 0_1ins405 |
| 12 | human | 302 | ANXA2 | annexin A2 | XM_024449908.1 | 62.1% | 62.1% | 0_1ins405 |
| 13 | mouse | 12306 | Anxa2 | annexin A2 | NM_007585.3 | 85% | 92.7% | (many diffs) |
| 14 | mouse | 12306 | Anxa2 | annexin A2 | XM_006510796.2 | 85% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1140
- ORF length:
- 1071
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gggccgccag ctagcggggt gcggagacgc tgggaagaag gcttccttca 121 aaatgtctac tgttcacgaa atcctgtgca agctcagctt ggagggtgat cactctacac 181 ccccaagtgc atatgggtct gtcaaagcct atactaactt tgatgctgag cgggatgctt 241 tgaacattga aacagccatc aagaccaaag gtgtggatga ggtcaccatt gtcaacattt 301 tgaccaaccg cagcaatgca cagagacagg atattgcctt cgcctaccag agaaggacca 361 aaaaggaact tgcatcagca ctgaagtcag ccttatctgg ccacctggag acggtgattt 421 tgggcctatt gaagacacct gctcagtatg acgcttctga gctaaaagct tccatgaagg 481 ggctgggaac cgacgaggac tctctcattg agatcatctg ctccagaacc aaccaggagc 541 tgcaggaaat taacagagtc tacaaggaaa tgtacaagac tgatctggag aaggacatta 601 tttcggacac atctggtgac ttccgcaagc tgatggttgc cctggcaaag ggtagaagag 661 cagaggatgg ctctgtcatt gattatgaac tgattgacca agatgctcgg gatctctatg 721 acgctggagt gaagaggaaa ggaactgatg ttcccaagtg gatcagcatc atgaccgagc 781 ggagcgtgcc ccacctccag aaagtatttG ATAGGTACAA GAGTTACAGC CCTTATGACA 841 TGTTGGAAAG CATCAGGAAA GAGGTTAAAG GAGACCTGGA AAATGCTTTC CTGAACCTGG 901 TTCAGTGCAT TCAGAACAAG CCCCTGTATT TTGCTGATCG GCTGTATGAC TCCATGAAGG 961 GCAAGGGGAC GCGAGATAAG GTCCTGATCA GAATCATGGT CTCCCGCAGT GAAGTGGACA 1021 TGTTGAAAAT TAGGTCTGAA TTCAAGAGAA AGTACGGCAA GTCCCTGTAC TATTATATCC 1081 AGCAAGACAC TAAGGGCGAC TACCAGAAAG CGCTGCTGTA CCTGTGTGGT GGAGATGACT 1141 AGAACCCAGC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1201 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1261 GGCTTTATAT ATCTTGTGGA AAGGACGAAT GCTAGCTATT GCAATTGCTA GAACGCGTTA 1321 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt