Construct: ORF TRCN0000491580

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018928.3_s317c1
Derived from:
ccsbBroadEn_15152
DNA Barcode:
AATTGGATTGTCGATGTGTTGATT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PEAK1 (79834)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491580
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79834 PEAK1 pseudopodium enriched atypi... NM_024776.4 90.7% 6.2% (many diffs)
2 human 79834 PEAK1 pseudopodium enriched atypi... XM_005254670.2 90.7% 6.2% (many diffs)
3 human 79834 PEAK1 pseudopodium enriched atypi... XM_005254671.2 90.7% 6.2% (many diffs)
4 human 79834 PEAK1 pseudopodium enriched atypi... XM_005254673.2 90.7% 6.2% (many diffs)
5 human 79834 PEAK1 pseudopodium enriched atypi... XM_005254674.2 90.7% 6.2% (many diffs)
6 human 79834 PEAK1 pseudopodium enriched atypi... XM_005254675.4 90.7% 6.2% (many diffs)
7 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522033.2 90.7% 6.2% (many diffs)
8 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522034.2 90.7% 6.2% (many diffs)
9 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522035.2 90.7% 6.2% (many diffs)
10 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522036.2 90.7% 6.2% (many diffs)
11 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522037.2 90.7% 6.2% (many diffs)
12 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522038.2 90.7% 6.2% (many diffs)
13 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522039.2 90.7% 6.2% (many diffs)
14 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450056.1 90.7% 6.2% (many diffs)
15 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450057.1 90.7% 6.2% (many diffs)
16 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450058.1 90.7% 6.2% (many diffs)
17 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450059.1 90.7% 6.2% (many diffs)
18 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450060.1 90.7% 6.2% (many diffs)
19 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450061.1 90.7% 6.2% (many diffs)
20 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450062.1 90.7% 6.2% (many diffs)
21 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450063.1 90.7% 6.2% (many diffs)
22 human 79834 PEAK1 pseudopodium enriched atypi... XM_024450064.1 90.7% 6.2% (many diffs)
23 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522040.1 69.3% 7.9% (many diffs)
24 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522041.1 51.6% 10.3% (many diffs)
25 human 79834 PEAK1 pseudopodium enriched atypi... XM_011522042.2 41.5% 3.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
480
ORF length:
411
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaan
61tnggcaccat gtctgcttgt aacaccttta ctgaacatgt ttggaaacct ggtgaatgca
121agaattgctt taaacctaaa agtttgcacc agcttccccc agaccctgag aaggcaccca
181tcacccatgg caatgtgaaa actaatgcca atcacagtaa caaccaccgc atcaggaaca
241cgggcaattt ccggcctcct gtggctaaaa aacccactat agctgtgaag cccactatga
301tagtggcaga tgggcaaagt atatgtggtg agcttagcat ccaagaacac tgtgagaaca
361aacctgtcat catagggtgg aaccgaaaag cggatcctca tttcagacgc caaaggcatc
421ctccagtgtc tgctctgggg cccccgcgaa gatctcttcc agactttcac cgcctgccct
481agcctagtac agaggaacac cctgctccaa aactggctag acatcaagcg aacactgctc
541atgatcaagt ttgctgagaa gtccctggac agggaaggtg gaatcagcct tgaggactgg
601ctttgtgctc agtatttggc ttttgccact acagactccc tcagttgtat tgtgaaaatt
661ctgcagcacc gtttgccaac tttcttgtac aaagttggca ttataagaaa gcnnnnnttn
721ncnanntgtt gcaacgaaca ggtcactatc agtcaaaata aaatcattat tgccatcnng
781nnngattcaa agttggcatt ataagaaagc anccccctgc ttatcaattt gttgcaacga
841acaggtcact atcagtcaaa ataaaatcat tatttgccat ccaggctgat atccccgtat
901agtgagtcgt attacatgga tcatagctgt ttcctggcag ctctgggccc gtgtctcaat
961ccacatcacc agattcttct ttaacagaag aatcacgttc tgagacagcc agtagtttat
1021cccagaagat ttgtaatggg ggattatctc ctggtaaccc aggagattct aaggacatga
1081aggaaattga gcccaattat gaaagtccct ctagtaataa tcaggataaa gattcatcac
1141aggcttccaa aagctcaata aaagttccag agacccacaa agcagtcctt gctctccgat
1201tagaagagaa agatggcaag attgctgtac aaactgagaa ggaagaaagt aaagcctcta
1261cagatgttgc tgggcaagca gtaaccataa accttgtccc cacagaagag caagcaaaac
1321cttaccgagt tgtgaacctg gaacagccat tgtgcaagcc atatactgtc gtggatgtgt
1381cagcagccat ggccagtgag cacctcgagg gccctgttaa cagccccaag acaaaaagct
1441catcctctac tccaaactct ccagttacat catcttcatt gacaccagga caaataagtg
1501cccatttcca aaaatccagt gcaattcgat accaagaagt atggacttct agcaccagtc
1561cacgacaaaa gatacctaaa gtagaactaa ttactagtgg aactggacca aatgttcctc
1621caaggaaaaa ctgtcacaaa tcagcaccta catcacccac agctacaaac atttcctcca
1681aaaccatccc tgttaagtca cctaatttgt ctgaaattaa atttaatagt tataacaatg
1741ctggtatgcc accttttcca attatcattc atgacgagcc aacttatgct cggagttcca
1801aaaatgctat caaagttccc attgttatca atccaaatgc atatgacaat ctagctatct
1861acaaaagttt tctgggaaca agtggagaac tctcagtgaa ggaaaaaacc acaagtgtaa
1921taagccatac ttatgaagaa atagaaacag aaagcaaagt gcctgataac accactagca
1981aaaccactga ctgtcttcaa actaaagggt tttcaaacag cacagagcat aaaaggggct
2041cagtggctca gaaggttcaa gagtttaaca actgtctcaa cagaggtcag tcttcaccac
2101agagaagcta tagttccagc cacagctccc cagcaaagat ccagagagcc actcaagagc
2161ctgtggccaa aatagaaggc actcaggagt ctcagatggt gggcagcagc agcaccagag
2221agaaagcaag cacagtgctt tctcagattg tggcttcaat ccaaccccca cagtctcctc
2281cagaaacacc tcaatctggc cctaaagctt gcagtgtgga agagctttat gccattcctc
2341cagatgctga tgttgctaag agcacaccta agagtacgcc agtccggccc aaatctctct
2401ttacatctca gcctagtggt gaggctgaag cacctcagac cacagacagt cctaccacca
2461aagtacagaa agacccatcc ataaagccag tcaccccctc tccctccaaa ttagtgacta
2521gcccccaaag tgagccacca gctccctttc ccccgccacg ctctacttct tctccttacc
2581atgcaggtaa ccttttgcag aggcatttca ccaactggac caagccaacc agccctacca
2641ggtcaacaga agctgaatca gttttgcact ctgaaggcag caggcgggca gctgatgcaa
2701aacctaagcg ctggatatca tttaaaagct tcttccgccg tcggaaaaca gatgaggagg
2761atgacaaaga gaaagagcga gagaaaggga aactggtggg cctggatggc acagtcattc
2821acatgctgcc tcctcctcca gttcagcgcc atcactggtt cacagaggcg aaaggagagt
2881ccagtgagaa accagccatt gtcttcatgt acaggtgcga ccctgctcaa ggccagctca
2941gtgtggatca gagcaaggct aggacagacc aggcagcagt catggagaag ggtagagcag
3001agaatgcatt actacaggac tcagagaaga agaggagtca ttcttctcca tcacagattc
3061ctaaaaagat tctcagtcac atgacccatg aagtaacaga ggatttttct cctcgggatc
3121caagaactgt tgttgggaag caagatggca ggggctgcac ttcagtcaca acagcattgt
3181ccctacctga actggaaagg gaagatggaa aagaagacat ttcagatcct atggacccga
3241acccttgtag tgcaacatac agcaacttag ggcaatctag agcagccatg atacctccca
3301agcagccacg acagcccaag ggagctgtgg acgatgccat cgcctttgga gggaaaacag
3361accaagaagc acccaatgct tcccaaccta caccaccccc actgccaaag aagatgatca
3421taagagccaa tacagagcca atctccaagg acctccaaaa atccatggaa agtagtcttt
3481gtgtcatggc taatcccacc tatgatatcg accccaactg ggatgccagc agtgctggtt
3541cttccatcag ctatgaactc aaaggactgg acattgagtc ttatgactcc ttggaaaggc
3601ctttgcgcaa ggagagacct gtcccctcag cagcaaacag catttccagc ttaaccactc
3661tcagtattaa ggatagattt tccaacagca tggaatccct ctccagccgg cgtgggccct
3721cttgcagaca gggccgaggc atccagaagc cgcagagaca agcactttat cgaggacttg
3781agaatcggga ggaagtagtg ggtaaaatcc gaagccttca tacagatgcc ttgaagaaac
3841tggctgttaa atgcgaagac cttttcatgg ctgggcagaa agaccagctc cgttttggag
3901tggacagctg gtcagacttc aggctaacca gtgacaaacc atgttgtgag gcaggtgatg
3961cggtttacta tactgcttca tatgcaaaag atccacttaa taactatgca gtcaagatct
4021gtaagagcaa agctaaagaa tctcagcagt attatcacag cttggctgtc cggcagagtc
4081tggctgtcca ttttaacatt cagcaggact gtggtcattt ccttgctgaa gtccctaacc
4141gtctgcttcc ctgggaggat ccagatgacc ctgaaaagga tgaggatgac atggaagaga
4201ctgaagaaga cgccaaagga gaaacggatg ggaaaaaccc aaagccctgt tctgaagcag
4261catcatccca gaaagagaat cagggagtca tgagcaagaa gcagaggagc cacgttgtgg
4321tcatcaccag ggaggttcca tgtcttactg tggctgattt tgtgcgagac tctctggccc
4381agcatgggaa aagccctgat ttgtatgaga ggcaggtgtg tctgctgctc ttacagctat
4441gctctggtct tgagcacctc aaaccctacc atgtcactca ctgcgatcta cgcctagaga
4501acctgctact tgtccactac cagcctgggg ggactgccca aggctttggg cctgcagagc
4561ccagccccac ctcatcttat cccactaggc ttatagtgag caacttctct caggccaagc
4621agaagagcca tctggtggac cccgagatcc tccgggacca gtctcgcctt gccccagaga
4681tcataacagc tacccagtat aaaaagtgtg atgagttcca gacaggcatc ctcatctatg
4741agatgctgca cctacccaac ccctttgatg agaacccaga gctgaaggag agggaataca
4801cacgagcaga cctgcctcgc atcccattcc gctcccccta ctcccggggt ctgcagcagc
4861tggccagctg cctcctgaat cccaaccctt ctgagcggat cctcatttca gacgccaaag
4921gcatccTCCA GTGTCTGCTC TGGGGCCCCC GCGAAGATCT CTTCCAGACT TTCACCGCCT
4981GCCCTAGCCT AGTACAGAGG AACACCCTGC TCCAAAACTG GCTAGACATC AAGCGAACAC
5041TGCTCATGAT CAAGTTTGCT GAGAAGTCCC TGGACAGGGA AGGTGGAATC AGCCTTGAGG
5101ACTGGCTTTG TGCTCAGTAT TTGGCTTTTG CCACTACAGA CTCCCTCAGT TGTATTGTGA
5161AAATTCTGCA GCACCGTTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
5221TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
5281GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAAATTGGA TTGTCGATGT
5341GTTGATTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)