Construct: ORF TRCN0000491585
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020592.2_s317c1
- DNA Barcode:
- GTTTGTAATAGCTAGATAGAACCT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ALPK1 (80216)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491585
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80216 | ALPK1 | alpha kinase 1 | NM_001102406.2 | 100% | 100% | |
| 2 | human | 80216 | ALPK1 | alpha kinase 1 | NM_025144.4 | 100% | 100% | |
| 3 | human | 80216 | ALPK1 | alpha kinase 1 | NM_001253884.1 | 93.7% | 92.6% | 0_1ins79;41_42ins155 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3804
- ORF length:
- 3732
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgaataat caaaaagtgg tagctgtgct actgcaagag tgcaagcaag 121 tgctggatca gctcttgttg gaagcgccag atgtgtcgga agaggacaag agcgaggacc 181 agcgctgcag agctttactc cccagcgagt taaggaccct gatccaggag gcaaaggaaa 241 tgaagtggcc cttcgtgcct gaaaagtggc agtacaaaca agccgtgggc ccagaggaca 301 aaacaaacct gaaggatgtg attggcgccg ggttgcagca gttactggcg tccctgaggg 361 cctccatcct cgctcgggac tgtgcggctg cggcggctat tgtgttcttg gtggaccggt 421 tcctgtatgg gctcgacgtc tctggaaaac ttctgcaggt cgccaaaggt ctccacaagt 481 tgcagccagc cacgccaatt gccccgcagg tggttattcg ccaagcccga atctccgtga 541 actcaggaaa acttttaaaa gcagagtata ttctgagcag tctaataagc aacaatggag 601 caacgggtac ctggctgtac agaaatgaaa gtgacaaggt cctggtgcag tcggtctgta 661 tacagatcag agggcagatt ctgcaaaagc tggggatgtg gtacgaagca gcagagttaa 721 tatgggcctc cattgtagga tatttggcac ttcctcagcc ggataaaaag ggcctctcca 781 cgtcgctagg tatactggca gacatctttg tttccatgag caagaacgat tatgaaaagt 841 ttaaaaacaa tccacaaatt aatttgagcc tgctgaagga gtttgaccac catttgctgt 901 ccgctgcaga agcctgcaag ctggcagctg ccttcagtgc ctatacgccg ctcttcgtgc 961 tcacagctgt gaatatccgt ggcacgtgtt tattgtccta cagtagttca aatgactgtc 1021 ctccagaatt gaaaaactta catctgtgtg aagccaaaga ggcctttgag attggcctcc 1081 tcaccaagag agatgatgag cctgttactg gaaaacagga gcttcacagc tttgtcaaag 1141 ctgctttcgg tctcaccaca gtgcacagaa ggctccatgg ggagacaggg acggtccatg 1201 cagcaagtca gctctgtaag gaagcaatgg ggaagctgta caatttcagc acttcctcca 1261 gaagtcagga cagagaagct ctgtctcaag aagttatgtc tgtgattgcc caggtgaagg 1321 aacatttaca agttcaaagc ttctcaaatg tagatgacag atcttatgtt cccgagagtt 1381 tcgagtgcag gttggataaa cttatcttgc atgggcaagg ggatttccaa aaaatccttg 1441 acacctattc acagcaccat acttcggtgt gtgaagtatt tgaaagtgat tgtggaaaca 1501 acaaaaatga acagaaagat gcaaaaacag gagtctgcat cactgctcta aaaacagaaa 1561 taaaaaacat agatactgtg agtactactc aagaaaagcc acattgtcaa agagacacag 1621 gaatatcttc ctccctaatg ggtaagaatg ttcagaggga actcagaagg ggaggaagga 1681 gaaactggac ccattctgat gcatttcgag tctccttgga tcaagatgtg gagactgaga 1741 ctgagccatc ggactacagc aatggtgagg gagctgtttt caacaagtct ctgagtggca 1801 gccagacttc cagtgcttgg agcaacttat cagggtttag ttcctctgca agctgggagg 1861 aagtgaatta tcacgttgac gacaggtcag ccagaaaaga gcctggcaaa gaacatctgg 1921 tggacactca gtgttccact gccttgtctg aggagctaga gaatgacagg gaaggcagag 1981 ctatgcattc attgcattca cagcttcatg atctctctct tcaggaaccc aacaatgaca 2041 atttggagcc ttctcaaaat cagccacagc aacagatgcc cttgacaccc ttctcgcctc 2101 ataatacccc aggcattttc ttggcccctg gtgcagggct tctagaagga gctccagaag 2161 gtatccagga agtcagaaat atgggaccca gaaatacttc tgctcactcc agaccctcat 2221 atcgttctgc ttcttggtct tctgattctg gtaggcccaa gaatatgggc acacatcctt 2281 cagtccaaaa agaagaagcc tttgaaataa ttgttgagtt tccagaaacc aactgcgatg 2341 tcaaagacag gcaggggaaa gagcagggag aagaaattag tgaaagaggc gcaggcccta 2401 catttaaagc tagtccctcc tgggttgacc cagaaggaga aacagcagaa agcactgaag 2461 atgcaccctt agactttcac agggtcctgc acaattctct gggaaacatt tccatgctgc 2521 catgtagctc cttcacccct aattggcctg ttcaaaatcc tgactccaga aaaagtggtg 2581 gcccagtcgc agagcagggc atcgaccctg atgcctccac agtggatgag gaggggcaac 2641 tgctcgacag catggatgtt ccctgcacaa atgggcacgg ctctcataga ctgtgcattc 2701 tgagacagcc gcctggtcag agggcggaga cccccaattc ctctgtaagc ggtaacatcc 2761 tcttccctgt cctcagcgag gactgcacta ccacagagga aggaaatcag cctggaaaca 2821 tgctaaactg cagccagaac tccagctcat cctcagtgtg gtggctgaaa tcacctgcat 2881 tttccagtgg ttcttctgag ggggacagcc cttggtccta tctgaattcc agtgggagtt 2941 cttgggtttc attgccggga aagatgagga aagagatcct tgaggctcgc accttgcaac 3001 ctgatgactt tgaaaagctg ttggcaggag tgaggcatga ttggctgttt cagagactag 3061 agaatacggg ggtttttaag cccagtcaac tccaccgagc acatagtgct cttttgttaa 3121 aatattcaaa aaaatctgaa ctgtggacgg cccaggaaac tattgtctat ttgggggact 3181 acttgactgt gaagaaaaaa ggcagacaaa gaaatgcttt ttgggttcat catcttcatc 3241 aagaagaaat tctggggagg tatgttggga aagactataa ggagcagaag gggctctggc 3301 accacttcac tgatgtggag cgacagatga ccgcacagca ctatgtgaca gaatttaaca 3361 agagactcta tgaacaaaac attcccaccc agatattcta catcccatcc acaatactac 3421 tgattttaga ggacaagaca ataaagggat gtatcagtgt ggagccttac atactGGGAG 3481 AATTTGTAAA ATTGTCAAAT AACACGAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT 3541 ATGGCTTGGC CTATGGCCAT TTTTCTTATG AGTTTTCTAA TCATAGAGAT GTTGTGGTCG 3601 ATTTACAAGG TTGGGTAACC GGTAATGGAA AAGGACTCAT CTACCTCACA GATCCCCAGA 3661 TTCACTCCGT TGATCAGAAA GTTTTCACTA CCAATTTTGG AAAGAGAGGA ATTTTTTACT 3721 TCTTTAATAA CCAGCATGTG GAATGTAATG AAATCTGCCA TCGTCTTTCT TTGACTAGAC 3781 CTTCAATGGA GAAACCATGC ACATAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG 3841 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 3901 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTTTGTAA 3961 TAGCTAGATA GAACCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 4021 aagatt