Construct: ORF TRCN0000491585

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020592.2_s317c1
DNA Barcode:
GTTTGTAATAGCTAGATAGAACCT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
ALPK1 (80216)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491585
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80216 ALPK1 alpha kinase 1 NM_001102406.2 100% 100%
2 human 80216 ALPK1 alpha kinase 1 NM_025144.4 100% 100%
3 human 80216 ALPK1 alpha kinase 1 NM_001253884.1 93.7% 92.6% 0_1ins79;41_42ins155
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
3804
ORF length:
3732
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaataat caaaaagtgg tagctgtgct actgcaagag tgcaagcaag
121tgctggatca gctcttgttg gaagcgccag atgtgtcgga agaggacaag agcgaggacc
181agcgctgcag agctttactc cccagcgagt taaggaccct gatccaggag gcaaaggaaa
241tgaagtggcc cttcgtgcct gaaaagtggc agtacaaaca agccgtgggc ccagaggaca
301aaacaaacct gaaggatgtg attggcgccg ggttgcagca gttactggcg tccctgaggg
361cctccatcct cgctcgggac tgtgcggctg cggcggctat tgtgttcttg gtggaccggt
421tcctgtatgg gctcgacgtc tctggaaaac ttctgcaggt cgccaaaggt ctccacaagt
481tgcagccagc cacgccaatt gccccgcagg tggttattcg ccaagcccga atctccgtga
541actcaggaaa acttttaaaa gcagagtata ttctgagcag tctaataagc aacaatggag
601caacgggtac ctggctgtac agaaatgaaa gtgacaaggt cctggtgcag tcggtctgta
661tacagatcag agggcagatt ctgcaaaagc tggggatgtg gtacgaagca gcagagttaa
721tatgggcctc cattgtagga tatttggcac ttcctcagcc ggataaaaag ggcctctcca
781cgtcgctagg tatactggca gacatctttg tttccatgag caagaacgat tatgaaaagt
841ttaaaaacaa tccacaaatt aatttgagcc tgctgaagga gtttgaccac catttgctgt
901ccgctgcaga agcctgcaag ctggcagctg ccttcagtgc ctatacgccg ctcttcgtgc
961tcacagctgt gaatatccgt ggcacgtgtt tattgtccta cagtagttca aatgactgtc
1021ctccagaatt gaaaaactta catctgtgtg aagccaaaga ggcctttgag attggcctcc
1081tcaccaagag agatgatgag cctgttactg gaaaacagga gcttcacagc tttgtcaaag
1141ctgctttcgg tctcaccaca gtgcacagaa ggctccatgg ggagacaggg acggtccatg
1201cagcaagtca gctctgtaag gaagcaatgg ggaagctgta caatttcagc acttcctcca
1261gaagtcagga cagagaagct ctgtctcaag aagttatgtc tgtgattgcc caggtgaagg
1321aacatttaca agttcaaagc ttctcaaatg tagatgacag atcttatgtt cccgagagtt
1381tcgagtgcag gttggataaa cttatcttgc atgggcaagg ggatttccaa aaaatccttg
1441acacctattc acagcaccat acttcggtgt gtgaagtatt tgaaagtgat tgtggaaaca
1501acaaaaatga acagaaagat gcaaaaacag gagtctgcat cactgctcta aaaacagaaa
1561taaaaaacat agatactgtg agtactactc aagaaaagcc acattgtcaa agagacacag
1621gaatatcttc ctccctaatg ggtaagaatg ttcagaggga actcagaagg ggaggaagga
1681gaaactggac ccattctgat gcatttcgag tctccttgga tcaagatgtg gagactgaga
1741ctgagccatc ggactacagc aatggtgagg gagctgtttt caacaagtct ctgagtggca
1801gccagacttc cagtgcttgg agcaacttat cagggtttag ttcctctgca agctgggagg
1861aagtgaatta tcacgttgac gacaggtcag ccagaaaaga gcctggcaaa gaacatctgg
1921tggacactca gtgttccact gccttgtctg aggagctaga gaatgacagg gaaggcagag
1981ctatgcattc attgcattca cagcttcatg atctctctct tcaggaaccc aacaatgaca
2041atttggagcc ttctcaaaat cagccacagc aacagatgcc cttgacaccc ttctcgcctc
2101ataatacccc aggcattttc ttggcccctg gtgcagggct tctagaagga gctccagaag
2161gtatccagga agtcagaaat atgggaccca gaaatacttc tgctcactcc agaccctcat
2221atcgttctgc ttcttggtct tctgattctg gtaggcccaa gaatatgggc acacatcctt
2281cagtccaaaa agaagaagcc tttgaaataa ttgttgagtt tccagaaacc aactgcgatg
2341tcaaagacag gcaggggaaa gagcagggag aagaaattag tgaaagaggc gcaggcccta
2401catttaaagc tagtccctcc tgggttgacc cagaaggaga aacagcagaa agcactgaag
2461atgcaccctt agactttcac agggtcctgc acaattctct gggaaacatt tccatgctgc
2521catgtagctc cttcacccct aattggcctg ttcaaaatcc tgactccaga aaaagtggtg
2581gcccagtcgc agagcagggc atcgaccctg atgcctccac agtggatgag gaggggcaac
2641tgctcgacag catggatgtt ccctgcacaa atgggcacgg ctctcataga ctgtgcattc
2701tgagacagcc gcctggtcag agggcggaga cccccaattc ctctgtaagc ggtaacatcc
2761tcttccctgt cctcagcgag gactgcacta ccacagagga aggaaatcag cctggaaaca
2821tgctaaactg cagccagaac tccagctcat cctcagtgtg gtggctgaaa tcacctgcat
2881tttccagtgg ttcttctgag ggggacagcc cttggtccta tctgaattcc agtgggagtt
2941cttgggtttc attgccggga aagatgagga aagagatcct tgaggctcgc accttgcaac
3001ctgatgactt tgaaaagctg ttggcaggag tgaggcatga ttggctgttt cagagactag
3061agaatacggg ggtttttaag cccagtcaac tccaccgagc acatagtgct cttttgttaa
3121aatattcaaa aaaatctgaa ctgtggacgg cccaggaaac tattgtctat ttgggggact
3181acttgactgt gaagaaaaaa ggcagacaaa gaaatgcttt ttgggttcat catcttcatc
3241aagaagaaat tctggggagg tatgttggga aagactataa ggagcagaag gggctctggc
3301accacttcac tgatgtggag cgacagatga ccgcacagca ctatgtgaca gaatttaaca
3361agagactcta tgaacaaaac attcccaccc agatattcta catcccatcc acaatactac
3421tgattttaga ggacaagaca ataaagggat gtatcagtgt ggagccttac atactGGGAG
3481AATTTGTAAA ATTGTCAAAT AACACGAAAG TGGTGAAAAC AGAATACAAA GCCACAGAAT
3541ATGGCTTGGC CTATGGCCAT TTTTCTTATG AGTTTTCTAA TCATAGAGAT GTTGTGGTCG
3601ATTTACAAGG TTGGGTAACC GGTAATGGAA AAGGACTCAT CTACCTCACA GATCCCCAGA
3661TTCACTCCGT TGATCAGAAA GTTTTCACTA CCAATTTTGG AAAGAGAGGA ATTTTTTACT
3721TCTTTAATAA CCAGCATGTG GAATGTAATG AAATCTGCCA TCGTCTTTCT TTGACTAGAC
3781CTTCAATGGA GAAACCATGC ACATAGGACC CAGCTTTCTT GTACAAAGTG GTTGATATCG
3841GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
3901AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGTTTGTAA
3961TAGCTAGATA GAACCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
4021aagatt

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