Construct: ORF TRCN0000491616
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020417.2_s317c1
- DNA Barcode:
- GCCATTATCAGAACCCAAGTTTCC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- PAK4 (10298)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491616
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014831.3 | 100% | 100% | |
| 2 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014832.2 | 100% | 100% | |
| 3 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_005884.4 | 100% | 100% | |
| 4 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526316.1 | 100% | 100% | |
| 5 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526317.2 | 100% | 100% | |
| 6 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526318.2 | 100% | 100% | |
| 7 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526319.2 | 100% | 100% | |
| 8 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014834.3 | 74.1% | 74.1% | 202_203ins459 |
| 9 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014835.2 | 74.1% | 74.1% | 202_203ins459 |
| 10 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526320.1 | 74.1% | 74.1% | 202_203ins459 |
| 11 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_024451314.1 | 74.1% | 74.1% | 202_203ins459 |
| 12 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | NM_027470.3 | 86.2% | 92.5% | (many diffs) |
| 13 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | XM_017312296.1 | 86.2% | 92.5% | (many diffs) |
| 14 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | XM_017312297.1 | 86.2% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 84
- ORF end:
- 1857
- ORF length:
- 1773
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggctccgc ggcccccttc accatgtttg ggaagaggaa gaagcgggtg gagatctccg 121 cgccgtccaa cttcgagcac cgcgtgcaca cgggcttcga ccagcacgag cagaagttca 181 cggggctgcc ccgccagtgg cagagcctga tcgaggagtc ggctcgccgg cccaagcccc 241 tcgtcgaccc cgcctgcatc acctccatcc agcccggggc ccccaagacc atcgtgcggg 301 gcagcaaagg tgccaaagat ggggccctca cgctgctgct ggacgagttt gagaacatgt 361 cggtgacacg ctccaactcc ctgcggagag acagcccgcc gccgcccgcc cgtgcccgcc 421 aggaaaatgg gatgccagag gagccggcca ccacggccag agggggccca gggaaggcag 481 gcagccgagg ccggttcgcc ggtcacagcg aggcgggtgg cggcagtggt gacaggcgac 541 gggcggggcc agagaagagg cccaagtctt ccagggaggg ctcagggggt ccccaggagt 601 cctcccggga caaacgcccc ctctccgggc ctgatgtcgg caccccccag cctgctggtc 661 tggccagtgg ggcgaaactg gcagctggcc ggccctttaa cacctacccg agggctgaca 721 cggaccaccc atcccggggt gcccaggggg agcctcatga cgtggcccct aacgggccat 781 cagcgggggg cctggccatc ccccagtcct cctcctcctc ctcccggcct cccacccgag 841 cccgaggtgc ccccagccct ggagtgctgg gaccccacgc ctcagagccc cagctggccc 901 ctccagcctg cacccccgcc gcccctgctg ttcctgggcc ccctggcccc cgctcaccac 961 agcgggagcc acagcgagta tcccatgagc agttccgggc tgccctgcag ctggtggtgg 1021 acccaggcga cccccgctcc tacctggaca acttcatcaa gattggcgag ggctccacgg 1081 gcatcgtgtg catcgccacc gtgcgcagct cgggcaagct ggtggccgtc aagaagatgg 1141 acctgcgcaa gcagcagagg cgcgagctgc tcttcaacga ggtggtaatc atgagggact 1201 accagcacga gaatgtggtg gagatgtaca acagctacct ggtgggggac gagctctggg 1261 tggtcatgga gttcctggaa ggaggcgccc tcaccgacat cgtcacccac accaggatga 1321 acgaggagca gatcgcggcc gtgtgccttg cagtgctgca ggccctgtcg gtgctccacg 1381 cccagggcgt catccaccgg gacatcaaga gcgactcgat cctgctgacc catgatggca 1441 gggtgaagct gtcagacttt gggttctgcg cccaggtgag caaggaagtg ccccgaagga 1501 agtcgctggt cggcacgccc tactggatgg ccccagagct catctcccgc cttccctacg 1561 ggccagaggt agacatctgg tcgctgggga taatggtgat tgagatggtg gacggagagc 1621 ccccctactt caacgagcca cccctcaaag ccatgaagat gattcgggac aacctgccac 1681 cccgactgaa gaacctgcac aaggtgtcgc catccctgaa gggcttcctg gaccgccTGC 1741 TGGTGCGAGA CCCTGCCCAG CGGGCCACGG CAGCCGAGCT GCTGAAGCAC CCATTCCTGG 1801 CCAAGGCAGG GCCGCCTGCC AGCATCGTGC CCCTCATGCG CCAGAACCGC ACCAGATGAG 1861 GCCCAGCGCC CTTCCCCTCA ACCAAAGAGC CCCCCGGGTC ACCCCCGCCC CACAAGGGTG 1921 GGCGCGCCGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1981 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 2041 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGCCATT ATCAGAACCC AAGTTTCCAC 2101 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt