Construct: ORF TRCN0000491625
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010460.2_s317c1
- Derived from:
- ccsbBroadEn_07036
- DNA Barcode:
- GACTCGGAGTCTTCTAGGCAACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCF4 (6925)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491625
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6925 | TCF4 | transcription factor 4 | NM_001083962.2 | 99.9% | 100% | 1941A>G |
| 2 | human | 6925 | TCF4 | transcription factor 4 | NM_001369567.1 | 99.9% | 100% | 1941A>G |
| 3 | human | 6925 | TCF4 | transcription factor 4 | NM_001369568.1 | 99.9% | 100% | 1941A>G |
| 4 | human | 6925 | TCF4 | transcription factor 4 | NM_001330604.3 | 99.8% | 99.8% | 1066_1067insCAG;1938A>G |
| 5 | human | 6925 | TCF4 | transcription factor 4 | NM_001369569.1 | 99.8% | 99.8% | 366_367insCAG;1938A>G |
| 6 | human | 6925 | TCF4 | transcription factor 4 | NM_001369570.1 | 99.8% | 99.8% | 366_367insCAG;1938A>G |
| 7 | human | 6925 | TCF4 | transcription factor 4 | NM_001369571.1 | 99.3% | 99.4% | 1633_1634insCTAGGTCAAGAT;1929A>G |
| 8 | human | 6925 | TCF4 | transcription factor 4 | NM_001369572.1 | 99.3% | 99.4% | 1633_1634insCTAGGTCAAGAT;1929A>G |
| 9 | human | 6925 | TCF4 | transcription factor 4 | NM_003199.3 | 99.3% | 99.4% | 1633_1634insCTAGGTCAAGAT;1929A>G |
| 10 | human | 6925 | TCF4 | transcription factor 4 | NM_001369573.1 | 99.2% | 99.2% | 366_367insCAG;1630_1631insCTAGGTCAAGAT;1926A>G |
| 11 | human | 6925 | TCF4 | transcription factor 4 | NM_001369574.1 | 99.2% | 99.2% | 1066_1067insCAG;1630_1631insCTAGGTCAAGAT;1926A>G |
| 12 | human | 6925 | TCF4 | transcription factor 4 | NM_001243228.2 | 99% | 99.1% | 550_567del;1959A>G |
| 13 | human | 6925 | TCF4 | transcription factor 4 | NM_001243227.2 | 96.3% | 96.4% | 0_1ins72;1869A>G |
| 14 | human | 6925 | TCF4 | transcription factor 4 | NM_001348217.1 | 96.3% | 96.4% | 0_1ins72;1869A>G |
| 15 | human | 6925 | TCF4 | transcription factor 4 | NM_001348218.2 | 96.3% | 96.4% | 0_1ins72;1869A>G |
| 16 | human | 6925 | TCF4 | transcription factor 4 | NM_001369575.1 | 96.3% | 96.4% | 0_1ins72;1869A>G |
| 17 | human | 6925 | TCF4 | transcription factor 4 | NM_001243230.1 | 96.3% | 96.2% | (many diffs) |
| 18 | human | 6925 | TCF4 | transcription factor 4 | NM_001369586.1 | 96.2% | 96.1% | 0_1ins72;998_1000delGTA;1872A>G |
| 19 | human | 6925 | TCF4 | transcription factor 4 | NM_001369576.1 | 96.2% | 96.2% | 0_1ins72;294_295insCAG;1866A>G |
| 20 | human | 6925 | TCF4 | transcription factor 4 | NM_001369577.1 | 96.2% | 96.2% | 0_1ins72;994_995insCAG;1866A>G |
| 21 | human | 6925 | TCF4 | transcription factor 4 | NM_001369578.1 | 96.2% | 96.2% | 0_1ins72;294_295insCAG;1866A>G |
| 22 | human | 6925 | TCF4 | transcription factor 4 | NM_001369579.1 | 96.2% | 96.2% | 0_1ins72;994_995insCAG;1866A>G |
| 23 | human | 6925 | TCF4 | transcription factor 4 | NM_001369580.1 | 96.2% | 96.2% | 0_1ins72;994_995insCAG;1866A>G |
| 24 | human | 6925 | TCF4 | transcription factor 4 | NM_001369581.1 | 96.2% | 96.2% | 0_1ins72;294_295insCAG;1866A>G |
| 25 | human | 6925 | TCF4 | transcription factor 4 | XM_006722538.3 | 96.2% | 96.2% | 0_1ins72;294_295insCAG;1866A>G |
| 26 | human | 6925 | TCF4 | transcription factor 4 | NM_001306207.1 | 95.7% | 95.8% | 0_1ins72;1561_1562insCTAGGTCAAGAT;1857A>G |
| 27 | human | 6925 | TCF4 | transcription factor 4 | NM_001348219.2 | 95.7% | 95.8% | 0_1ins72;1561_1562insCTAGGTCAAGAT;1857A>G |
| 28 | human | 6925 | TCF4 | transcription factor 4 | NM_001369582.1 | 95.7% | 95.8% | 0_1ins72;1561_1562insCTAGGTCAAGAT;1857A>G |
| 29 | human | 6925 | TCF4 | transcription factor 4 | NM_001369583.1 | 95.7% | 95.8% | 0_1ins72;1561_1562insCTAGGTCAAGAT;1857A>G |
| 30 | human | 6925 | TCF4 | transcription factor 4 | NM_001348220.1 | 95.6% | 95.6% | (many diffs) |
| 31 | human | 6925 | TCF4 | transcription factor 4 | NM_001369584.1 | 95.6% | 95.6% | (many diffs) |
| 32 | human | 6925 | TCF4 | transcription factor 4 | NM_001369585.1 | 95.6% | 95.6% | (many diffs) |
| 33 | human | 6925 | TCF4 | transcription factor 4 | XM_017025950.2 | 95.4% | 95.5% | (many diffs) |
| 34 | human | 6925 | TCF4 | transcription factor 4 | NM_001348211.2 | 93.4% | 92.6% | (many diffs) |
| 35 | human | 6925 | TCF4 | transcription factor 4 | XM_017025951.2 | 93.3% | 92.5% | (many diffs) |
| 36 | human | 6925 | TCF4 | transcription factor 4 | NM_001243231.2 | 92.8% | 92.1% | (many diffs) |
| 37 | human | 6925 | TCF4 | transcription factor 4 | XM_024451241.1 | 88.1% | 88.2% | 0_1ins237;1704A>G |
| 38 | human | 6925 | TCF4 | transcription factor 4 | NM_001243226.3 | 86.7% | 86.8% | 1_306del;2247A>G |
| 39 | human | 6925 | TCF4 | transcription factor 4 | XM_005266749.4 | 86.7% | 82.9% | (many diffs) |
| 40 | human | 6925 | TCF4 | transcription factor 4 | NM_001243232.1 | 86.5% | 82.8% | (many diffs) |
| 41 | human | 6925 | TCF4 | transcription factor 4 | XM_017025952.2 | 86.4% | 82.7% | (many diffs) |
| 42 | human | 6925 | TCF4 | transcription factor 4 | XM_017025953.2 | 86.1% | 82.4% | (many diffs) |
| 43 | human | 6925 | TCF4 | transcription factor 4 | NM_001306208.1 | 86% | 82.2% | (many diffs) |
| 44 | human | 6925 | TCF4 | transcription factor 4 | NM_001330605.3 | 80.5% | 80.6% | 0_1ins390;1551A>G |
| 45 | human | 6925 | TCF4 | transcription factor 4 | NM_001348213.2 | 80.5% | 80.6% | 0_1ins390;1551A>G |
| 46 | human | 6925 | TCF4 | transcription factor 4 | XM_005266752.5 | 80.5% | 80.6% | 0_1ins390;1551A>G |
| 47 | human | 6925 | TCF4 | transcription factor 4 | XM_005266755.5 | 80.5% | 80.6% | 0_1ins390;1551A>G |
| 48 | human | 6925 | TCF4 | transcription factor 4 | XM_017025954.2 | 80.4% | 80.4% | 0_1ins390;676_677insCAG;1548A>G |
| 49 | human | 6925 | TCF4 | transcription factor 4 | NM_001243233.2 | 79.9% | 80% | 0_1ins390;1243_1244insCTAGGTCAAGAT;1539A>G |
| 50 | human | 6925 | TCF4 | transcription factor 4 | NM_001348212.2 | 79.9% | 80% | 0_1ins390;1243_1244insCTAGGTCAAGAT;1539A>G |
| 51 | human | 6925 | TCF4 | transcription factor 4 | XM_017025956.2 | 79.9% | 80% | 0_1ins390;1243_1244insCTAGGTCAAGAT;1539A>G |
| 52 | human | 6925 | TCF4 | transcription factor 4 | NM_001348216.2 | 75.2% | 72.5% | (many diffs) |
| 53 | human | 6925 | TCF4 | transcription factor 4 | NM_001243236.2 | 74.6% | 71.9% | (many diffs) |
| 54 | human | 6925 | TCF4 | transcription factor 4 | NM_001243234.2 | 74.4% | 71.9% | (many diffs) |
| 55 | human | 6925 | TCF4 | transcription factor 4 | XM_005266761.4 | 74.3% | 71.8% | (many diffs) |
| 56 | human | 6925 | TCF4 | transcription factor 4 | NM_001243235.2 | 73.8% | 71.3% | (many diffs) |
| 57 | human | 6925 | TCF4 | transcription factor 4 | NM_001348214.2 | 73.7% | 71.2% | (many diffs) |
| 58 | human | 6925 | TCF4 | transcription factor 4 | NM_001348215.2 | 67.7% | 67.8% | 0_1ins648;1293A>G |
| 59 | mouse | 21413 | Tcf4 | transcription factor 4 | NM_013685.2 | 92.4% | 98% | (many diffs) |
| 60 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525746.3 | 92.4% | 98% | (many diffs) |
| 61 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525747.3 | 92.4% | 98% | (many diffs) |
| 62 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525748.3 | 92.3% | 97.9% | (many diffs) |
| 63 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525749.3 | 92.3% | 97.9% | (many diffs) |
| 64 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317854.1 | 92.3% | 97.9% | (many diffs) |
| 65 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317855.1 | 92.3% | 97.9% | (many diffs) |
| 66 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317856.1 | 92.2% | 97.7% | (many diffs) |
| 67 | mouse | 21413 | Tcf4 | transcription factor 4 | NM_001083967.1 | 91.9% | 97.4% | (many diffs) |
| 68 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525750.3 | 91.9% | 97.4% | (many diffs) |
| 69 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317857.1 | 91.9% | 97.4% | (many diffs) |
| 70 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525751.3 | 91.7% | 97.3% | (many diffs) |
| 71 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525752.3 | 91.7% | 97.3% | (many diffs) |
| 72 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317858.1 | 91.6% | 97.1% | (many diffs) |
| 73 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525753.3 | 88.9% | 94.4% | (many diffs) |
| 74 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317859.1 | 88.7% | 94.3% | (many diffs) |
| 75 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525757.3 | 73.9% | 79.1% | (many diffs) |
| 76 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525759.3 | 73.9% | 79.1% | (many diffs) |
| 77 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317860.1 | 73.9% | 79.1% | (many diffs) |
| 78 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317863.1 | 73.9% | 79.1% | (many diffs) |
| 79 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317861.1 | 73.3% | 78.5% | (many diffs) |
| 80 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525760.3 | 69% | 70.6% | (many diffs) |
| 81 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525766.3 | 68.8% | 70.4% | (many diffs) |
| 82 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525762.3 | 68.4% | 70% | (many diffs) |
| 83 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525761.3 | 68.4% | 71.2% | (many diffs) |
| 84 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525763.3 | 67.8% | 70.6% | (many diffs) |
| 85 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_006525764.3 | 61.8% | 66.6% | (many diffs) |
| 86 | mouse | 21413 | Tcf4 | transcription factor 4 | XM_017317862.1 | 61.2% | 66% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2082
- ORF length:
- 2013
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcatcaccaa cagcgaatgg ctgccttagg gacggacaaa gagctgagtg 121 atttactgga tttcagtgcg atgttttcac ctcctgtgag cagtgggaaa aatggaccaa 181 cttctttggc aagtggacat tttactggct caaatgtaga agacagaagt agctcagggt 241 cctgggggaa tggaggacat ccaagcccgt ccaggaacta tggagatggg actccctatg 301 accacatgac cagcagggac cttgggtcac atgacaatct ctctccacct tttgtcaatt 361 ccagaataca aagtaaaaca gaaaggggct catactcatc ttatgggaga gaatcaaact 421 tacagggttg ccaccagcag agtctccttg gaggtgacat ggatatgggc aacccaggaa 481 ccctttcgcc caccaaacct ggttcccagt actatcagta ttctagcaat aatccccgaa 541 ggaggcctct tcacagtagt gccatggagg tacagacaaa gaaagttcga aaagttcctc 601 caggtttgcc atcttcagtc tatgctccat cagcaagcac tgccgactac aatagggact 661 cgccaggcta tccttcctcc aaaccagcaa ccagcacttt ccctagctcc ttcttcatgc 721 aagatggcca tcacagcagt gacccttgga gctcctccag tgggatgaat cagcctggct 781 atgcaggaat gttgggcaac tcttctcata ttccacagtc cagcagctac tgtagcctgc 841 atccacatga acgtttgagc tatccatcac actcctcagc agacatcaat tccagtcttc 901 ctccgatgtc cactttccat cgtagtggta caaaccatta cagcacctct tcctgtacgc 961 ctcctgccaa cgggacagac agtataatgg caaatagagg aagcggggca gccggcagct 1021 cccagactgg agatgctctg gggaaagcac ttgcttcgat ctattctcca gatcacacta 1081 acaacagctt ttcatcaaac ccttcaactc ctgttggctc tcctccatct ctctcagcag 1141 gcacagctgt ttggtctaga aatggaggac aggcctcatc gtctcctaat tatgaaggac 1201 ccttacactc tttgcaaagc cgaattgaag atcgtttaga aagactggat gatgctattc 1261 atgttctccg gaaccatgca gtgggcccat ccacagctat gcctggtggt catggggaca 1321 tgcatggaat cattggacct tctcataatg gagccatggg tggtctgggc tcagggtatg 1381 gaaccggcct tctttcagcc aacagacatt cactcatggt ggggacccat cgtgaagatg 1441 gcgtggccct gagaggcagc cattctcttc tgccaaacca ggttccggtt ccacagcttc 1501 ctgtccagtc tgcgacttcc cctgacctga acccacccca ggacccttac agaggcatgc 1561 caccaggact acaggggcag agtgtctcct ctggcagctc tgagatcaaa tccgatgacg 1621 agggtgatga gaacctgcaa gacacgaaat cttcggagga caagaaatta gatgacgaca 1681 agaaggatat caaatcaatt actaggtcaa gatctagcaa taatgacgat gaggaCCTGA 1741 CACCAGAGCA GAAGGCAGAG CGTGAGAAGG AGCGGAGGAT GGCCAACAAT GCCCGAGAGC 1801 GTCTGCGGGT CCGTGACATC AACGAGGCTT TCAAAGAGCT CGGCCGCATG GTGCAGCTCC 1861 ACCTCAAGAG TGACAAGCCC CAGACCAAGC TCCTGATCCT CCACCAGGCG GTGGCCGTCA 1921 TCCTCAGTCT GGAGCAGCAA GTCCGAGAAA GGAATCTGAA TCCGAAAGCT GCGTGTCTGA 1981 AAAGAAGGGA GGAAGAGAAG GTGTCCTCGG AGCCTCCCCC TCTCTCCTTG GCCGGCCCAC 2041 ACCCTGGAAT GGGAGACGCA TCGAATCACA TGGGACAGAT GTTGCCAACT TTCTTGTACA 2101 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2161 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2221 AGGACGAGAC TCGGAGTCTT CTAGGCAACC TACGCGTTAA GTCgacaatc aacctctgga 2281 ttacaaaatt tgtgaaagat t