Transcript: Human NM_001369568.1

Homo sapiens transcription factor 4 (TCF4), transcript variant 28, mRNA.

Source:
NCBI, updated 2019-08-27
Taxon:
Homo sapiens (human)
Gene:
TCF4 (6925)
Length:
8034
CDS:
169..2184

Additional Resources:

NCBI RefSeq record:
NM_001369568.1
NBCI Gene record:
TCF4 (6925)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001369568.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000274162 CAACGGGACAGACAGTATAAT pLKO_005 1068 CDS 100% 15.000 21.000 N TCF4 n/a
2 TRCN0000274161 CTATCAGTATTCTAGCAATAA pLKO_005 612 CDS 100% 13.200 18.480 N TCF4 n/a
3 TRCN0000360448 GTTTCAGCATTCCCAATTATC pLKO_005 2335 3UTR 100% 13.200 18.480 N Tcf4 n/a
4 TRCN0000235556 TAGGTCAAGATCTAGCAATAA pLKO_005 1803 CDS 100% 13.200 18.480 N Tcf4 n/a
5 TRCN0000274213 GAGACTGAACGGCAATCTTTC pLKO_005 2469 3UTR 100% 10.800 15.120 N TCF4 n/a
6 TRCN0000015037 CACGAAATCTTCGGAGGACAA pLKO.1 1743 CDS 100% 4.050 5.670 N TCF4 n/a
7 TRCN0000274214 CACGAAATCTTCGGAGGACAA pLKO_005 1743 CDS 100% 4.050 5.670 N TCF4 n/a
8 TRCN0000015036 GAAAGGAATCTGAATCCGAAA pLKO.1 2047 CDS 100% 4.050 3.240 N TCF4 n/a
9 TRCN0000274163 GAAAGGAATCTGAATCCGAAA pLKO_005 2047 CDS 100% 4.050 3.240 N TCF4 n/a
10 TRCN0000235554 TGTTACTTGTGATGCAATTAA pLKO_005 6008 3UTR 100% 15.000 10.500 N Tcf4 n/a
11 TRCN0000012094 GCTGAGTGATTTACTGGATTT pLKO.1 213 CDS 100% 10.800 7.560 N Tcf4 n/a
12 TRCN0000015035 CGAATTGAAGATCGTTTAGAA pLKO.1 1321 CDS 100% 5.625 3.938 N TCF4 n/a
13 TRCN0000015033 CCACATAAACACTTCTCCTTA pLKO.1 2256 3UTR 100% 4.950 3.465 N TCF4 n/a
14 TRCN0000015034 GCAGACATCAATTCCAGTCTT pLKO.1 979 CDS 100% 4.950 3.465 N TCF4 n/a
15 TRCN0000012096 CCCAGTACTATCAGTATTCAA pLKO.1 605 CDS 100% 5.625 3.375 N Tcf4 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001369568.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07036 pDONR223 100% 99.9% 100% None 1941A>G n/a
2 ccsbBroad304_07036 pLX_304 26.8% 99.9% 100% V5 1941A>G n/a
3 TRCN0000491625 GACTCGGAGTCTTCTAGGCAACCT pLX_317 17.5% 99.9% 100% V5 1941A>G n/a
4 TRCN0000488472 GACCCCTACTCCGCTAGGAGTACC pLX_317 14.8% 99.4% 99.4% V5 (not translated due to prior stop codon) 1634_1645del n/a
5 ccsbBroadEn_15607 pDONR223 0% 99.3% 99.4% None 1634_1645del;1941A>G n/a
6 ccsbBroad304_15607 pLX_304 0% 99.3% 99.4% V5 1634_1645del;1941A>G n/a
7 TRCN0000479737 GCCATGAGCTAGGTAGAGGATTAG pLX_317 21.2% 99.3% 99.2% V5 1634_1645del;1765A>G;1941A>G n/a
8 ccsbBroadEn_11176 pDONR223 100% 54.3% 54.3% None 1096_2013del n/a
9 ccsbBroad304_11176 pLX_304 0% 54.3% 54.3% V5 1096_2013del n/a
10 TRCN0000469284 ACGCAAGGCACAAAAGCACTCACT pLX_317 41.6% 54.3% 54.3% V5 1096_2013del n/a
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