Construct: ORF TRCN0000491687
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000440.1_s317c1
- Derived from:
- ccsbBroadEn_03564
- DNA Barcode:
- AATACCTCGCAAGCCACCGTGAGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR41 (55255)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491687
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55255 | WDR41 | WD repeat domain 41 | NM_018268.4 | 100% | 100% | |
| 2 | human | 55255 | WDR41 | WD repeat domain 41 | XM_011543505.2 | 97.9% | 95.9% | (many diffs) |
| 3 | human | 55255 | WDR41 | WD repeat domain 41 | XM_017009606.1 | 97.8% | 96.9% | (many diffs) |
| 4 | human | 55255 | WDR41 | WD repeat domain 41 | XM_005248551.4 | 87.3% | 87.3% | 522_523ins174 |
| 5 | human | 55255 | WDR41 | WD repeat domain 41 | XM_005248552.3 | 79.9% | 79.9% | 1101_1102ins276 |
| 6 | human | 55255 | WDR41 | WD repeat domain 41 | XM_011543507.2 | 73.1% | 72.9% | 1006_1008delTTTinsCAG;1011_1011delTins367 |
| 7 | human | 55255 | WDR41 | WD repeat domain 41 | XR_001742140.1 | 60% | 1_287del;1665_2292del | |
| 8 | human | 55255 | WDR41 | WD repeat domain 41 | XR_001742141.1 | 31.9% | 1_287del;1379_1400del;1687_4304del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1443
- ORF length:
- 1377
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gcgatggctg atcgggggag gccgagaacc gcagggactg gccgagaaat 121 ctcctttaca gacaataggt gaagaacaaa cccagaatcc ctacactgaa ctgctagtac 181 tgaaggctca tcatgatatt gtacgatttc tggtacagtt agatgactac agatttgcat 241 ctgctggtga tgatggaatt gtagttgtgt ggaatgccca gacaggggaa aaacttttag 301 aactgaatgg acacactcaa aagataacag ctattattac atttccttcc ttggaatctt 361 gtgaagagaa aaatcaactc atcttgacag cctctgctga tagaacagtt attgtgtggg 421 atggtgatac taccagacaa gttcagagaa tatcatgctt ccagtctact gtaaagtgtt 481 taactgttct tcagagacta gatgtttggc tttctggtgg gaatgacctg tgtgtgtgga 541 accgaaaatt agatctcctg tgtaagacta gccacctttc tgatacaggt attagtgctt 601 tggttgaaat acctaagaac tgtgttgtgg cagcagttgg caaagaactg ataattttca 661 ggttggtagc acccacagaa ggatcactag aatgggatat tcttgaagtt aagcgcctcc 721 ttgatcacca ggataatatt ctctcattga ttaatgtcaa tgatttgagt tttgtcaccg 781 gctcccacgt cggagagctg atcatctggg atgccctgga ctggaccatg caggcctatg 841 aacgcaactt ctgggaccca tctccacaac tggacaccca acaagaaata aaactctgtc 901 aaaaatcaaa tgacatttct attcatcatt tcacatgtga tgaagagaat gtatttgctg 961 cagttggaag gggtttatac gtgtatagcc ttcaaatgaa gcgtgtgatt gcctgccaga 1021 aaactgcaca tgactccaat gtcctgcacg ttgccagact tccaaacagg cagttaatct 1081 catgctcaga agatGGCAGT GTACGCATTT GGGAGTTAAG AGAAAAACAG CAGCTTGCAG 1141 CTGAGCCTGT ACCAACAGGT TTTTTTAACA TGTGGGGATT TGGAAGAGTC AGCAAACAAG 1201 CCAGCCAACC TGTTAAAAAG CAGCAAGAAA ATGCTACTTC ATGTTCACTG GAGCTTATTG 1261 GAGATTTGAT TGGACACTCA TCATCTGTGG AGATGTTTCT ATACTTTGAA GATCATGGAC 1321 TAGTGACGTG CTCCGCTGAT CATCTCATTA TTTTGTGGAA AAATGGAGAG CGAGAATCTG 1381 GATTGCGCAG TTTAAGATTA TTTCAAAAAT TAGAGGAGAA TGGTGACTTA TACCTTGCTG 1441 TCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1501 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1561 GGCTTTATAT ATCTTGTGGA AAGGACGAAA TACCTCGCAA GCCACCGTGA GTACGCGTTA 1621 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt