Construct: ORF TRCN0000491692
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF019505.2_s317c1
- DNA Barcode:
- AAAGCATGTTTTGACTTAAAGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAK4 (10298)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491692
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014831.3 | 100% | 100% | |
| 2 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014832.2 | 100% | 100% | |
| 3 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_005884.4 | 100% | 100% | |
| 4 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526316.1 | 100% | 100% | |
| 5 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526317.2 | 100% | 100% | |
| 6 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526318.2 | 100% | 100% | |
| 7 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526319.2 | 100% | 100% | |
| 8 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014834.3 | 74.1% | 74.1% | 202_203ins459 |
| 9 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | NM_001014835.2 | 74.1% | 74.1% | 202_203ins459 |
| 10 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_011526320.1 | 74.1% | 74.1% | 202_203ins459 |
| 11 | human | 10298 | PAK4 | p21 (RAC1) activated kinase 4 | XM_024451314.1 | 74.1% | 74.1% | 202_203ins459 |
| 12 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | NM_027470.3 | 86.2% | 92.5% | (many diffs) |
| 13 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | XM_017312296.1 | 86.2% | 92.5% | (many diffs) |
| 14 | mouse | 70584 | Pak4 | p21 protein (Cdc42/Rac)-act... | XM_017312297.1 | 86.2% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 102
- ORF end:
- 1875
- ORF length:
- 1773
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgga tcccaccgag tccccggcac catgtttggg aagaggaaga 121 agcgggtgga gatctccgcg ccgtccaact tcgagcaccg cgtgcacacg ggcttcgacc 181 agcacgagca gaagttcacg gggctgcccc gccagtggca gagcctgatc gaggagtcgg 241 ctcgccggcc caagcccctc gtcgaccccg cctgcatcac ctccatccag cccggggccc 301 ccaagaccat cgtgcggggc agcaaaggtg ccaaagatgg ggccctcacg ctgctgctgg 361 acgagtttga gaacatgtcg gtgacacgct ccaactccct gcggagagac agcccgccgc 421 cgcccgcccg tgcccgccag gaaaatggga tgccagagga gccggccacc acggccagag 481 ggggcccagg gaaggcaggc agccgaggcc ggttcgccgg tcacagcgag gcgggtggcg 541 gcagtggtga caggcgacgg gcggggccag agaagaggcc caagtcttcc agggagggct 601 cagggggtcc ccaggagtcc tcccgggaca aacgccccct ctccgggcct gatgtcggca 661 ccccccagcc tgctggtctg gccagtgggg cgaaactggc agctggccgg ccctttaaca 721 cctacccgag ggctgacacg gaccacccat cccggggtgc ccagggggag cctcatgacg 781 tggcccctaa cgggccatca gcggggggcc tggccatccc ccagtcctcc tcctcctcct 841 cccggcctcc cacccgagcc cgaggtgccc ccagccctgg agtgctggga ccccacgcct 901 cagagcccca gctggcccct ccagcctgca cccccgccgc ccctgctgtt cctgggcccc 961 ctggcccccg ctcaccacag cgggagccac agcgagtatc ccatgagcag ttccgggctg 1021 ccctgcagct ggtggtggac ccaggcgacc cccgctccta cctggacaac ttcatcaaga 1081 ttggcgaggg ctccacgggc atcgtgtgca tcgccaccgt gcgcagctcg ggcaagctgg 1141 tggccgtcaa gaagatggac ctgcgcaagc agcagaggcg cgagctgctc ttcaacgagg 1201 tggtaatcat gagggactac cagcacgaga atgtggtgga gatgtacaac agctacctgg 1261 tgggggacga gctctgggtg gtcatggagt tcctggaagg aggcgccctc accgacatcg 1321 tcacccacac caggatgaac gaggagcaga tcgcggccgt gtgccttgca gtgctgcagg 1381 ccctgtcggt gctccacgcc cagggcgtca tccaccggga catcaagagc gactcgatcc 1441 tgctgaccca tgatggcagg gtgaagctgt cagactttgg gttctgcgcc caggtgagca 1501 aggaagtgcc ccgaaggaag tcgctggtcg gcacgcccta ctggatggcc ccagagctca 1561 tctcccgcct tccctacggg ccagaggtag acatctggtc gctggggata atggtgattg 1621 agatggtgga cggagagccc cccTACTTCA ACGAGCCACC CCTCAAAGCC ATGAAGATGA 1681 TTCGGGACAA CCTGCCACCC CGACTGAAGA ACCTGCACAA GGTGTCGCCA TCCCTGAAGG 1741 GCTTCCTGGA CCGCCTGCTG GTGCGAGACC CTGCCCAGCG GGCCACGGCA GCCGAGCTGC 1801 TGAAGCACCC ATTCCTGGCC AAGGCAGGGC CGCCTGCCAG CATCGTGCCC CTCATGCGCC 1861 AGAACCGCAC CAGAAAGGGT GGGCGCGCCG ACCCAGCTTT CTTGTACAAA GTGGTTGATA 1921 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1981 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAAAGC 2041 ATGTTTTGAC TTAAAGCCTA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 2101 tgaaagatt