Construct: ORF TRCN0000491737
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021220.2_s317c1
- DNA Barcode:
- TACACAACTTACAGCATTCAAGTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SORCS3 (22986)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491737
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 22986 | SORCS3 | sortilin related VPS10 doma... | NM_014978.3 | 99.9% | 100% | 1368C>T |
| 2 | human | 22986 | SORCS3 | sortilin related VPS10 doma... | XM_011539542.2 | 70.8% | 70.8% | 0_1ins1068;300C>T |
| 3 | mouse | 66673 | Sorcs3 | sortilin-related VPS10 doma... | NM_025696.3 | 88.2% | 92.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3738
- ORF length:
- 3666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catggaggcg gcgcgcacgg agcgccccgc aggcaggccg ggggcgccgc 121 ttgtccggac ggggctccta ctcttgtcga cgtgggtcct ggccggcgcc gagatcactt 181 gggacgcgac aggcggtccc ggacgcccgg cggccccggc ttcgcggcca ccggcgttgt 241 ctccactctc gccgcgggca gtggccagcc agtggccgga ggagctggcg tcggcgcgga 301 gagccgccgt gctggggcgc cgggccggac cagagctgct gccccagcag ggcggcggca 361 gaggcggtga gatgcaggtg gaagccggag ggacatcacc ggcaggcgag cggcggggcc 421 ggggcatccc agctcctgcc aagcttggcg gcgcgaggag gagtcgccgg gcgcagcccc 481 caatcaccca ggaacgcggg gacgcctggg ccactgctcc ggccgatggt tccagaggaa 541 gccgtcccct tgctaagggt tcccgggagg aggtgaaggc gccgcgggct ggggggtcgg 601 cggctgaaga cctccggctg cccagcacct ccttcgcgct gaccggggac tcggcccaca 661 accaagccat ggtgcactgg tcgggacaca acagcagcgt catacttatc ctgacgaagc 721 tgtatgactt caacctgggc agcgtgactg agagttcact atggaggtcg acagattatg 781 gcaccaccta tgaaaagctg aatgacaaag tgggtttgaa gactgtcctc agttacctct 841 atgtcaatcc aaccaacaaa aggaagatta tgcttctcag tgatcctgag atggagagca 901 gcatattgat cagctcagac gaaggggcga cctatcagaa gtatcggctc accttctata 961 tccagagcct gctctttcat cccaagcaag aggactgggt gctggcctac agtttggatc 1021 aaaagctcta cagctccatg gactttggaa gacggtggca actcatgcat gaacgcatca 1081 cacccaacag gttttattgg tcggtggccg gattggataa ggaggcggac ctggtgcaca 1141 tggaggtgcg gaccacggat ggatatgctc actacctcac ctgcaggatc caggaatgtg 1201 ccgagacaac tagaagtggg ccttttgccc gctccattga catcagttcc ctggttgtcc 1261 aggatgaata tatcttcatt caggtaacaa ctagtggaag agccagctac tacgtgtctt 1321 atcgaagaga ggcctttgct cagataaagc tgcctaagta ctcgttgcca aaggacatgc 1381 acatcatcag tacagacgag aaccaagtat ttgctgcggt ccaagaatgg aaccagaatg 1441 acacgtacaa cctctacatc tcagacacgc gtgggattta cttcactctg gccatggaga 1501 acatcaagag cagcagaggt ctaatgggga acatcattat tgaattgtat gaggtagcag 1561 gtatcaaagg gatatttctg gcaaacaaga aggtggacga ccaggtgaag acatacatca 1621 cttacaacaa aggcagggat tggcgcctgc tgcaagctcc ggatgtggac ctgagaggaa 1681 gcccagtgca ctgcctgctg cccttctgtt ccttacatct gcacctgcaa ctctctgaaa 1741 atccatattc ctcaggaaga atctctagca aggagacagc cccaggactt gtggtggcta 1801 caggcaacat tggcccggag ctctcatata ctgatattgg tgtgttcatc tcctccgatg 1861 ggggcaacac atggagacag atctttgatg aagagtacaa tgtctggttc ctagactggg 1921 gtggtgccct cgtggccatg aaacacacac ctctgccagt caggcatttg tgggtgagtt 1981 ttgatgaggg ccactcttgg gacaagtatg gtttcacttc ggttcctctc tttgttgacg 2041 gggctctggt ggaggcagga atggagaccc acatcatgac agtttttggc cacttcagcc 2101 tccgctccga atggcaattg gtgaaagtgg actacaaatc tatcttcagc cggcattgca 2161 ccaaggagga ctatcagacc tggcacctgc tcaatcaggg agagccttgt gtcatgggag 2221 aaaggaaaat attcaagaaa cgtaagccag gagctcagtg tgccctgggc cgagaccact 2281 caggatcagt ggtctcagaa ccctgtgtct gtgccaattg ggacttcgag tgtgactatg 2341 ggtatgagag acatggggag agccagtgtg tcccagcttt ctggtacaat ccagcatccc 2401 catcaaagga ctgcagcctt ggtcaaagct accttaacag cactgggtat cggcggattg 2461 tgtccaacaa ctgcacagat gggctaaggg agaagtacac cgccaaggcc cagatgtgcc 2521 ctggaaaagc ccctcggggc ctccatgtgg tgacgaccga tgggcggctg gtggcagagc 2581 aggggcacaa tgcaactttc atcatcctca tggaggaggg tgatctacaa aggacaaaca 2641 tccagcttga ctttggggat gggattgctg tgtcctacgc aaacttcagc cccatcgagg 2701 acggcatcaa gcacgtgtat aagagtgcgg ggatcttcca ggtgacagcc tatgcagaga 2761 acaaccttgg ctcagacaca gctgtcctct tcctgcatgt ggtttgtcct gtggagcatg 2821 ttcatctccg agttccattt gttgccataa gaaataagga ggtcaacatc agtgcagtcg 2881 tgtggcccag tcaactgggg acccttacct atttctggtg gttcggcaat agcacaaagc 2941 ctctcatcac tttggacagc agcatttcct tcacattcct tgcagaagga accgacacca 3001 tcacagtcca ggtggctgct gggaatgccc tcatccagga cacaaaagag attgcagttc 3061 atgaatattt ccagtcccag cttttatcat tctctcctaa tctggattac cacaatcctg 3121 acattcctga gtggagaaaa gatattggca atgtcatcaa gcgagctctg gttaaagtaa 3181 ccagtgtccc agaggaccag atcctcattg ccgtgtttcc tggtctcccc acttcagcag 3241 agcttttcat tcttccaccc aagaacctga cagagaggag gaaaggcaat gaaggggacc 3301 tggaacaaat tgtagaaaca ctgtttaatg ctctcaacca aaatttggtc cagtttgagc 3361 tgaagccggg ggtacaagtc attgtgtatg tcacacagct gacgttagct ccattggtgg 3421 actccagtgc tgggcacagc agctcagcca tgcttatgct attatcagtg gtatttgttg 3481 gcctGGCTGT GTTTTTGATC TACAAGTTTA AAAGGAAAAT CCCTTGGATT AACATCTATG 3541 CTCAAGTCCA ACACGACAAG GAGCAGGAGA TGATTGGGTC AGTGAGCCAA AGTGAAAACG 3601 CCCCCAAAAT CACACTCAGT GACTTTACGG AGCCTGAGGA GCTGCTGGAC AAAGAGCTGG 3661 ACACGCGGGT CATAGGAGGC ATTGCCACTA TTGCAAACAG CGAAAGCACA AAGGAGATCC 3721 CCAACTGCAC TAGTGTTTAG AACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3781 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3841 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATACA CAACTTACAG 3901 CATTCAAGTC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt