Construct: ORF TRCN0000491739
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020669.2_s317c1
- DNA Barcode:
- ATTAAAAGAGAAATATGCATACGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- CDK14 (5218)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491739
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5218 | CDK14 | cyclin dependent kinase 14 | NM_012395.3 | 100% | 100% | |
| 2 | human | 5218 | CDK14 | cyclin dependent kinase 14 | NM_001287136.1 | 93.7% | 93.7% | 0_1ins84 |
| 3 | human | 5218 | CDK14 | cyclin dependent kinase 14 | NM_001287135.2 | 93.3% | 91.1% | (many diffs) |
| 4 | human | 5218 | CDK14 | cyclin dependent kinase 14 | NM_001287137.1 | 75.3% | 71.3% | 0_1ins238;77_78ins95 |
| 5 | mouse | 18647 | Cdk14 | cyclin-dependent kinase 14 | XM_006503544.4 | 86.3% | 92% | (many diffs) |
| 6 | mouse | 18647 | Cdk14 | cyclin-dependent kinase 14 | NM_011074.3 | 85.7% | 89.8% | (many diffs) |
| 7 | mouse | 18647 | Cdk14 | cyclin-dependent kinase 14 | NM_001310448.1 | 85.6% | 92.4% | (many diffs) |
| 8 | mouse | 18647 | Cdk14 | cyclin-dependent kinase 14 | XM_006503547.4 | 85.6% | 92.4% | (many diffs) |
| 9 | mouse | 18647 | Cdk14 | cyclin-dependent kinase 14 | XM_017320708.1 | 85.6% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 1425
- ORF length:
- 1353
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcacggt tactttggct gcaatgctgc tgcagagccc ggttactctg 121 ccttcgtggg aactccacag atatgtgtca caaagatgtc tacacggaac tgccagggaa 181 tggactcagt gatcaaaccc ctggacacaa ttcctgagga taaaaaagtc agagttcaga 241 ggacacagag cacttttgac ccatttgaga aaccagctaa tcaagtaaag agggtgcatt 301 ctgagaacaa tgcttgcatt aactttaaga cctcctccac tggcaaagag tcacctaaag 361 ttaggcggca ctccagcccc agctcgccaa caagtcccaa atttggaaaa gctgactcat 421 atgaaaagct ggaaaaacta ggggaaggat cttatgctac agtatacaaa gggaaaagca 481 aggtaaatgg gaagttggta gctctgaagg tgatcaggct gcaggaagaa gaagggacac 541 ctttcacagc tatcagggaa gcttctcttt taaaaggact aaaacatgct aacatagtgc 601 tacttcatga catcatccat accaaggaga cgctgacact tgtgtttgaa tatgtgcaca 661 ctgatttatg tcagtacatg gacaagcacc ctggggggct gcatccagat aatgtgaagt 721 tgtttttatt tcagttgctg cgaggtctgt cttacatcca ccagcgttat attttgcaca 781 gagacctgaa accacagaac cttctgatca gtgacacggg ggagttaaag ctggcagatt 841 tcggtcttgc aagagcaaaa tccgtcccta gccacacata ctccaacgaa gtggttacct 901 tgtggtacag acctccagat gtccttctag gctcaacaga atattccacc tgccttgaca 961 tgtggggagt aggttgcatc tttgttgaaa tgatccaagg agttgctgct tttccaggaa 1021 tgaaagacat tcaggatcaa cttgaacgaa tatttcTGGT TCTTGGAACA CCAAATGAGG 1081 ACACATGGCC TGGAGTTCAT TCTTTACCAC ATTTTAAGCC AGAACGCTTT ACCCTGTACA 1141 GCTCTAAAAA CCTTAGACAA GCATGGAATA AGCTCAGCTA TGTGAACCAT GCAGAGGACC 1201 TGGCCTCCAA GCTCCTACAA TGTTCCCCAA AGAACAGACT GTCGGCACAG GCTGCCTTGA 1261 GCCACGAGTA TTTTAGTGAC CTGCCGCCAC GGCTATGGGA ACTCACCGAC ATGTCTTCTA 1321 TTTTTACTGT CCCAAATGTG AGATTGCAAC CAGAAGCTGG AGAAAGCATG CGGGCCTTTG 1381 GGAAAAACAA TAGTTATGGC AAAAGTCTAT CAAACAGCAA GCACTAGAAC CCAGCTTTCT 1441 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1501 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1561 GTGGAAAGGA CGAATTAAAA GAGAAATATG CATACGCACG CGTTAAGTCg acaatcaacc 1621 tctggattac aaaatttgtg aaagatt