Construct: ORF TRCN0000491748
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF022031.2_s317c1
- DNA Barcode:
- ATTATGCAAACGGGTAGTGATCTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SLC28A1 (9154)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491748
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9154 | SLC28A1 | solute carrier family 28 me... | NM_001287762.2 | 99.8% | 100% | 1149G>A;1368A>G |
| 2 | human | 9154 | SLC28A1 | solute carrier family 28 me... | NM_004213.5 | 99.8% | 100% | 1149G>A;1368A>G |
| 3 | human | 9154 | SLC28A1 | solute carrier family 28 me... | NM_001321722.2 | 97% | 96% | (many diffs) |
| 4 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_017022723.1 | 97% | 96.1% | (many diffs) |
| 5 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522210.2 | 96.3% | 95.5% | (many diffs) |
| 6 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522203.2 | 92.2% | 89.5% | (many diffs) |
| 7 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522204.3 | 92.2% | 89.5% | (many diffs) |
| 8 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522205.3 | 92.2% | 89.5% | (many diffs) |
| 9 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522206.3 | 92.2% | 89.5% | (many diffs) |
| 10 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_024450099.1 | 92.2% | 89.5% | (many diffs) |
| 11 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522209.2 | 88.5% | 85.8% | (many diffs) |
| 12 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_024450100.1 | 88.5% | 85.8% | (many diffs) |
| 13 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_024450101.1 | 88.5% | 85.8% | (many diffs) |
| 14 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522208.3 | 85.4% | 81.2% | (many diffs) |
| 15 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522212.1 | 83.2% | 82% | (many diffs) |
| 16 | human | 9154 | SLC28A1 | solute carrier family 28 me... | NM_001321721.2 | 83% | 81.6% | (many diffs) |
| 17 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522214.2 | 82.1% | 81.6% | (many diffs) |
| 18 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522211.3 | 81% | 78.4% | (many diffs) |
| 19 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522216.2 | 81% | 78.4% | (many diffs) |
| 20 | human | 9154 | SLC28A1 | solute carrier family 28 me... | NM_001287761.1 | 74.4% | 74.4% | 1083_1084ins498 |
| 21 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522217.1 | 74.4% | 74.4% | 1083_1084ins498 |
| 22 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XR_931944.2 | 72.8% | (many diffs) | |
| 23 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522215.2 | 68.4% | 65.9% | (many diffs) |
| 24 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XR_002957690.1 | 56.5% | (many diffs) | |
| 25 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_011522218.2 | 55.7% | 55.6% | (many diffs) |
| 26 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XR_931945.2 | 53.6% | (many diffs) | |
| 27 | human | 9154 | SLC28A1 | solute carrier family 28 me... | XM_024450102.1 | 40.9% | 38.6% | (many diffs) |
| 28 | human | 9154 | SLC28A1 | solute carrier family 28 me... | NM_201651.3 | 26.2% | 23.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2016
- ORF length:
- 1947
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat ggagaacgac ccctcgagac gaagagagtc catctctctc acacctgtgg 121 ccaagggtct ggagaacatg ggggctgatt tcttggaaag cctggaggaa ggccagctcc 181 ctaggagtga cttgagcccc gcagagatca ggagcagctg gagcgaggcg gcgccgaagc 241 ccttctccag atggaggaac ctgcagccag ccctgagagc cagaagcttc tgcagggagc 301 acatgcagct gtttcgatgg atcggcacag gcctgctctg cactgggctc tctgccttcc 361 tgctggtggc ctgcctcctg gatttccaga gggccctggc tctgtttgtc ctcacctgtg 421 tggtcctcac cttcctgggc caccgcctgc tgaaacggct tctggggcca aagctgagga 481 ggtttctcaa gcctcagggc catccccgcc tgctgctctg gtttaagagg ggtctagctc 541 ttgctgcttt cctgggcctg gtcctgtggc tgtctctgga cacctcccag cggcctgagc 601 aactggtgtc cttcgcagga atctgcgtgt tcgtcgctct cctctttgcc tgctcaaagc 661 atcattgcgc agtgtcctgg agggccgtgt cttggggact tggactgcag tttgtacttg 721 gactcctcgt catcagaaca gaaccaggat tcattgcgtt cgagtggctg ggcgagcaga 781 tccggatctt cctgagctac acgaaggctg gctccagctt cgtgtttggg gaggcgctgg 841 tcaaggatgt ctttgccttt caggttctgc ccatcattgt ctttttcagc tgtgtcatat 901 ccgttctcta ccacgtgggc ctcatgcagt gggtgatcct gaagattgcc tggctgatgc 961 aagtcaccat gggcaccaca gccactgaga ccctgagtgt ggctggaaac atctttgtga 1021 gccagaccga ggctccatta ctgatccggc cctacttggc agacatgaca ctctctgaag 1081 tccacgttgt catgaccgga ggttacgcca ccattgctgg cagcctgctg ggtgcctaca 1141 tctcctttgg gatcgatgcc acctcgttga ttgcagcctc tgtgatggct gccccttgtg 1201 ccttggccct ctccaaactg gtctacccgg aggtggagga gtccaagttt aggagggagg 1261 aaggagtgaa actgacctat ggagatgctc agaacctcat agaagcagcc agcactgggg 1321 ccgccatctc cgtgaaggtg gtcgccaaca tcgctgccaa cctgattgcg ttcctggctg 1381 tgctggactt tatcaatgct gccctctcct ggctgggaga catggtggac atccaggggc 1441 tcagcttcca gctcatctgc tcctacatcc tgcggcctgt agccttcttg atgggtgtgg 1501 cgtgggagga ctgcccagtg gtagctgagc tgctggggat caagctgttt ctgaacgagt 1561 ttgtggccta tcaagacctc tccaagtaca agcaacgccg cctggcaggg gccgaggagt 1621 gggtcggcga caggaagcag tggatctccg tcagagctga agtcctcacg acgtttgccc 1681 tctgtggatt tgccaatttc agctccattg ggatcatgct gggaggcttg acctccatgg 1741 TCCCCCAACG GAAGAGCGAC TTCTCCCAGA TAGTGCTCCG GGCGCTCTTC ACGGGAGCCT 1801 GTGTGTCCCT GGTGAACGCC TGTATGGCAG GGATCCTCTA CATGCCCAGG GGGGCTGAAG 1861 TTGACTGCAT GTCCCTCTTG AACACGACCC TCAGCAGCAG TAGCTTTGAG ATTTACCAGT 1921 GCTGCCGTGA GGCCTTCCAG AGCGTCAATC CAGAGTTCAG CCCAGAGGCC CTGGACAACT 1981 GCTGTCGGTT TTACAACCAC ACGATCTGTG CACAGTGAGA CCCAGCTTTC TTGTACAAAG 2041 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2101 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2161 ACGAATTATG CAAACGGGTA GTGATCTTAC GCGTTAAGTC gacaatcaac ctctggatta 2221 caaaatttgt gaaagatt