Construct: ORF TRCN0000491764
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021945.2_s317c1
- DNA Barcode:
- CACTCATGTCGTGGCCCAAAAAAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- EGFR (1956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491764
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.3:c.2254_2277del | 99.9% | 100% | 2337G>A;3285C>A |
| 2 | human | 1956 | EGFR | epidermal growth factor rec... | NM_005228.5 | 99.2% | 99.3% | (many diffs) |
| 3 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346899.1 | 95.5% | 95.5% | (many diffs) |
| 4 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346900.2 | 94.8% | 94.9% | (many diffs) |
| 5 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346898.2 | 91.9% | 89.7% | (many diffs) |
| 6 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346897.2 | 88.2% | 85.9% | (many diffs) |
| 7 | human | 1956 | EGFR | epidermal growth factor rec... | NM_001346941.2 | 76.7% | 74.4% | (many diffs) |
| 8 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201284.2 | 56.5% | 51% | (many diffs) |
| 9 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201282.2 | 52.1% | 52.1% | 1881G>A;1883C>G;1884_1885ins1722 |
| 10 | human | 1956 | EGFR | epidermal growth factor rec... | NM_201283.1 | 33.6% | 33.5% | 1206_1207insG;1212_1213delGAinsTT;1215_1216ins2390 |
| 11 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_207655.2 | 84.1% | 89.6% | (many diffs) |
| 12 | mouse | 13649 | Egfr | epidermal growth factor rec... | NM_007912.4 | 45.2% | 46.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3678
- ORF length:
- 3606
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgcgaccc tccgggacgg ccggggcagc gctcctggcg ctgctggctg 121 cgctctgccc ggcgagtcgg gctctggagg aaaagaaagt ttgccaaggc acgagtaaca 181 agctcacgca gttgggcact tttgaagatc attttctcag cctccagagg atgttcaata 241 actgtgaggt ggtccttggg aatttggaaa ttacctatgt gcagaggaat tatgatcttt 301 ccttcttaaa gaccatccag gaggtggctg gttatgtcct cattgccctc aacacagtgg 361 agcgaattcc tttggaaaac ctgcagatca tcagaggaaa tatgtactac gaaaattcct 421 atgccttagc agtcttatct aactatgatg caaataaaac cggactgaag gagctgccca 481 tgagaaattt acaggaaatc ctgcatggcg ccgtgcggtt cagcaacaac cctgccctgt 541 gcaacgtgga gagcatccag tggcgggaca tagtcagcag tgactttctc agcaacatgt 601 cgatggactt ccagaaccac ctgggcagct gccaaaagtg tgatccaagc tgtcccaatg 661 ggagctgctg gggtgcagga gaggagaact gccagaaact gaccaaaatc atctgtgccc 721 agcagtgctc cgggcgctgc cgtggcaagt cccccagtga ctgctgccac aaccagtgtg 781 ctgcaggctg cacaggcccc cgggagagcg actgcctggt ctgccgcaaa ttccgagacg 841 aagccacgtg caaggacacc tgccccccac tcatgctcta caaccccacc acgtaccaga 901 tggatgtgaa ccccgagggc aaatacagct ttggtgccac ctgcgtgaag aagtgtcccc 961 gtaattatgt ggtgacagat cacggctcgt gcgtccgagc ctgtggggcc gacagctatg 1021 agatggagga agacggcgtc cgcaagtgta agaagtgcga agggccttgc cgcaaagtgt 1081 gtaacggaat aggtattggt gaatttaaag actcactctc cataaatgct acgaatatta 1141 aacacttcaa aaactgcacc tccatcagtg gcgatctcca catcctgccg gtggcattta 1201 ggggtgactc cttcacacat actcctcctc tggatccaca ggaactggat attctgaaaa 1261 ccgtaaagga aatcacaggg tttttgctga ttcaggcttg gcctgaaaac aggacggacc 1321 tccatgcctt tgagaaccta gaaatcatac gcggcaggac caagcaacat ggtcagtttt 1381 ctcttgcagt cgtcagcctg aacataacat ccttgggatt acgctccctc aaggagataa 1441 gtgatggaga tgtgataatt tcaggaaaca aaaatttgtg ctatgcaaat acaataaact 1501 ggaaaaaact gtttgggacc tccggtcaga aaaccaaaat tataagcaac agaggtgaaa 1561 acagctgcaa ggccacaggc caggtctgcc atgccttgtg ctcccccgag ggctgctggg 1621 gcccggagcc cagggactgc gtctcttgcc ggaatgtcag ccgaggcagg gaatgcgtgg 1681 acaagtgcaa ccttctggag ggtgagccaa gggagtttgt ggagaactct gagtgcatac 1741 agtgccaccc agagtgcctg cctcaggcca tgaacatcac ctgcacagga cggggaccag 1801 acaactgtat ccagtgtgcc cactacattg acggccccca ctgcgtcaag acctgcccgg 1861 caggagtcat gggagaaaac aacaccctgg tctggaagta cgcagacgcc ggccatgtgt 1921 gccacctgtg ccatccaaac tgcacctacg gatgcactgg gccaggtctt gaaggctgtc 1981 caacgaatgg gcctaagatc ccgtccatcg ccactgggat ggtgggggcc ctcctcttgc 2041 tgctggtggt ggccctgggg atcggcctct tcatgcgaag gcgccacatc gttcggaagc 2101 gcacgctgcg gaggctgctg caggagaggg agcttgtgga gcctcttaca cccagtggag 2161 aagctcccaa ccaagctctc ttgaggatct tgaaggaaac tgaattcaaa aagatcaaag 2221 tgctgggctc cggtgcgttc ggcacggtgt ataagggact ctggatccca gaaggtgaga 2281 aagttaaaat tcccgtcgct atcaaggaat taagagaagc aacactcgat gaagcctacg 2341 tgatggccag cgtggacaac ccccacgtgt gccgcctgct gggcatctgc ctcacctcca 2401 ccgtgcaact catcacgcag ctcatgccct tcggctgcct cctggactat gtccgggaac 2461 acaaagacaa tattggctcc cagtacctgc tcaactggtg tgtgcagatc gcaaagggca 2521 tgaactactt ggaggaccgt cgcttggtgc accgcgacct ggcagccagg aacgtactgg 2581 tgaaaacacc gcagcatgtc aagatcacag attttgggct ggccaaactg ctgggtgcgg 2641 aagagaaaga ataccatgca gaaggaggca aagtgcctat caagtggatg gcattggaat 2701 caattttaca cagaatctat acccaccaga gtgatgtctg gagctacggg gtgaccgttt 2761 gggagttgat gacctttgga tccaagccat atgacggaat ccctgccagc gagatctcct 2821 ccatcctgga gaaaggagaa cgcctccctc agccacccat atgtaccatc gatgtctaca 2881 tgatcatggt caagtgctgg atgatagacg cagatagtcg cccaaagttc cgtgagttga 2941 tcatcgaatt ctccaaaatg gcccgagacc cccagcgcta ccttgtcatt cagggggatg 3001 aaagaatgca tttgccaagt cctacagact ccaacttcta ccgtgccctg atggatgaag 3061 aagacatgga cgacgtggtg gatgccgacg agtacctcat cccacagcag ggcttcttca 3121 gcagcccctc cacgtcacgg actcccctcc tgagctctct gagtgcaacc agcaacaatt 3181 ccaccgtggc ttgcattgat agaaatgggc tgcaaagctg tcccatcaag gaagacagct 3241 tcttgcagcg atacagctca gaccccacag gcgccttgac tgaggacagc atagacgaca 3301 ccttcctccc agtgcctgaa tacataaacc agtccgttcc caaaaggccc gctggatctg 3361 tgcagaatcc tgtctatcac aatcagcctc tgaaccccgc gcccagcaga gacccacact 3421 accaggaccc ccacagcact gcagtgggca accccgagta tcTCAACACT GTCCAGCCCA 3481 CCTGTGTCAA CAGCACATTC GACAGCCCTG CCCACTGGGC CCAGAAAGGC AGCCACCAAA 3541 TTAGCCTGGA CAACCCTGAC TACCAGCAGG ACTTCTTTCC CAAGGAAGCC AAGCCAAATG 3601 GCATCTTTAA GGGCTCCACA GCTGAAAATG CAGAATACCT AAGGGTCGCG CCACAAAGCA 3661 GTGAATTTAT TGGAGCATGA GACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACACT CATGTCGTGG 3841 CCCAAAAAAT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt