Construct: ORF TRCN0000491795
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF020611.2_s317c1
- DNA Barcode:
- CTCAGGGGGTATCAGACGTGAACA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- FES (2242)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491795
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2242 | FES | FES proto-oncogene, tyrosin... | NM_002005.4 | 99.9% | 100% | 15C>T;504T>C |
| 2 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_017022005.1 | 99.9% | 100% | 15C>T;504T>C |
| 3 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_017022009.2 | 99.9% | 100% | 15C>T;504T>C |
| 4 | human | 2242 | FES | FES proto-oncogene, tyrosin... | NM_001143783.1 | 92.8% | 92.9% | 15C>T;211_212ins174;330T>C |
| 5 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_005254880.1 | 92.8% | 92.9% | 15C>T;211_212ins174;330T>C |
| 6 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_017022006.1 | 92.8% | 92.9% | 15C>T;211_212ins174;330T>C |
| 7 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_017022010.1 | 92.8% | 92.9% | 15C>T;211_212ins174;330T>C |
| 8 | human | 2242 | FES | FES proto-oncogene, tyrosin... | NM_001143784.1 | 91.4% | 91.4% | 15C>T;504T>C;1320_1321ins210 |
| 9 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_005254882.2 | 91.4% | 91.4% | 15C>T;504T>C;1320_1321ins210 |
| 10 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_017022007.1 | 91.4% | 91.4% | 15C>T;504T>C;1320_1321ins210 |
| 11 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XR_001751142.1 | 87.9% | (many diffs) | |
| 12 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XR_001751144.2 | 87% | (many diffs) | |
| 13 | human | 2242 | FES | FES proto-oncogene, tyrosin... | NM_001143785.2 | 84.3% | 84.4% | (many diffs) |
| 14 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XM_017022008.1 | 84.3% | 84.4% | (many diffs) |
| 15 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XR_001751143.1 | 80.4% | (many diffs) | |
| 16 | human | 2242 | FES | FES proto-oncogene, tyrosin... | XR_243206.3 | 79.6% | (many diffs) | |
| 17 | mouse | 14159 | Fes | feline sarcoma oncogene | NM_010194.2 | 87.2% | 90.6% | (many diffs) |
| 18 | mouse | 14159 | Fes | feline sarcoma oncogene | XM_006540604.3 | 87.2% | 90.6% | (many diffs) |
| 19 | mouse | 14159 | Fes | feline sarcoma oncogene | XM_006540605.2 | 48.7% | 43.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 2538
- ORF length:
- 2466
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggcttc tcttctgagc tgtgcagccc ccagggccac ggggtcctgc 121 agcaaatgca ggaggccgag cttcgtctac tggagggcat gagaaagtgg atggcccagc 181 gggtcaagag tgacagggag tatgcaggac tgcttcacca catgtccctg caggacagtg 241 ggggccagag ccgggccatc agccctgaca gccccatcag tcagtcctgg gctgagatca 301 ccagccaaac tgagggcctg agccgcttgc tgcggcagca cgcagaggat ctgaactcag 361 ggcccctgag caagctgagc ctgctcatcc gggaacggca gcagcttcgc aagacctaca 421 gcgagcagtg gcagcagctg cagcaggagc tcaccaagac ccacagccag gacattgaga 481 agctgaagag ccagtaccga gctctggcac gggacagtgc ccaagccaag cgcaagtacc 541 aggaggccag caaagacaag gaccgtgaca aggccaagga caagtatgtg cgcagcctgt 601 ggaagctctt tgctcaccac aaccgctatg tgctgggcgt gcgggctgcg cagctacacc 661 accagcacca ccaccagctc ctgctgcccg gcctgctgcg gtcactgcag gacctgcacg 721 aggagatggc ttgcatcctg aaggagatcc tgcaggaata cctggagatt agcagcctgg 781 tgcaggatga ggtggtggcc attcaccggg agatggctgc agctgctgcc cgcatccagc 841 ctgaggctga gtaccaaggc ttcctgcgac agtatgggtc cgcacctgac gtcccaccct 901 gtgtcacgtt cgatgagtca ctgcttgagg agggtgaacc gctggagcct ggggagctcc 961 agctgaacga gctgactgtg gagagcgtgc agcacacgct gacctcagtg acagatgagc 1021 tggctgtggc caccgagatg gtgttcaggc ggcaggagat ggttacgcag ctgcaacagg 1081 agctccggaa tgaagaggag aacacccacc cccgggagcg ggtgcagctg ctgggcaaga 1141 ggcaagtgct gcaagaagca ctgcaggggc tgcaggtagc gctgtgcagc caggccaagc 1201 tgcaggccca gcaggagttg ctgcagacca agctggagca cctgggcccc ggcgagcccc 1261 cgcctgtgct gctcctgcag gatgaccgcc actccacgtc gtcctcggag caggagcgag 1321 aggggggaag gacacccacg ctggagatcc ttaagagcca catctcagga atcttccgcc 1381 ccaagttctc gctccctcca ccgctgcagc tcattccgga ggtgcagaag cccctgcatg 1441 agcagctgtg gtaccacggg gccatcccga gggcagaggt ggctgagctg ctggtgcact 1501 ctggggactt cctggtgcgg gagagccagg gcaagcagga gtacgtgctg tcggtgctgt 1561 gggatggtct gccccggcac ttcatcatcc agtccttgga taacctgtac cgactggaag 1621 gggaaggctt tcctagcatt cctttgctca tcgaccacct actgagcacc cagcagcccc 1681 tcaccaagaa gagtggtgtt gtcctgcaca gggctgtgcc caaggacaag tgggtgctga 1741 accatgagga cctggtgttg ggtgagcaga ttggacgggg gaactttggc gaagtgttca 1801 gcggacgcct gcgagccgac aacaccctgg tggcggtgaa gtcttgtcga gagacgctcc 1861 cacctgacct caaggccaag tttctacagg aagcgaggat cctgaagcag tacagccacc 1921 ccaacatcgt gcgtctcatt ggtgtctgca cccagaagca gcccatctac atcgtcatgg 1981 agcttgtgca ggggggcgac ttcctgacct tcctccgcac ggagggggcc cgcctgcggg 2041 tgaagactct gctgcagatg gtgggggatg cagctgctgg catggagtac ctggagagca 2101 agtgctgcat ccaccgggac ctggctgctc ggaactgcct ggtgacagag aagaatgtcc 2161 tgaagatcag tgactttggg atgtcccgag aggaagccga tggggtctat gcagcctcag 2221 ggggcctcag acaagtcccc gtgaagtgga ccgcacctga ggcccttaac tacggccgct 2281 actcctccga aagcgacgtg tggagctttg gcatcttgct ctgggagacc ttcagcctgg 2341 gggcctcccc ctatcccaac cTCAGCAATC AGCAGACACG GGAGTTTGTG GAGAAGGGGG 2401 GCCGTCTGCC CTGCCCAGAG CTGTGTCCTG ATGCCGTGTT CAGGCTCATG GAGCAGTGCT 2461 GGGCCTATGA GCCTGGGCAG CGGCCCAGCT TCAGCACCAT CTACCAGGAG CTGCAGAGCA 2521 TCCGAAAGCG GCATCGGTGA AACCCAGCTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 2581 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 2641 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTCA GGGGGTATCA 2701 GACGTGAACA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt