Construct: ORF TRCN0000491812
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007542.1_s317c1
- Derived from:
- ccsbBroadEn_12497
- DNA Barcode:
- GATAGCCAGTTTGCATAGCTTGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP3A43 (64816)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491812
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64816 | CYP3A43 | cytochrome P450 family 3 su... | NM_001278921.2 | 100% | 100% | |
| 2 | human | 64816 | CYP3A43 | cytochrome P450 family 3 su... | XM_017012544.1 | 82% | 67.9% | (many diffs) |
| 3 | human | 64816 | CYP3A43 | cytochrome P450 family 3 su... | XM_017012545.1 | 82% | 67.9% | (many diffs) |
| 4 | human | 64816 | CYP3A43 | cytochrome P450 family 3 su... | XM_024446877.1 | 79.3% | 65.9% | (many diffs) |
| 5 | human | 64816 | CYP3A43 | cytochrome P450 family 3 su... | NM_057095.3 | 75.2% | 69.3% | (many diffs) |
| 6 | human | 64816 | CYP3A43 | cytochrome P450 family 3 su... | NM_022820.5 | 75.1% | 69.2% | (many diffs) |
| 7 | human | 64816 | CYP3A43 | cytochrome P450 family 3 su... | NR_103869.2 | 62.2% | (many diffs) | |
| 8 | human | 64816 | CYP3A43 | cytochrome P450 family 3 su... | NM_057096.4 | 58.6% | 52.5% | (many diffs) |
| 9 | human | 64816 | CYP3A43 | cytochrome P450 family 3 su... | NR_103868.2 | 52.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1248
- ORF length:
- 1179
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatctcatt ccaaactttg ccatggaaac atgggttctt gtggctacca 121 gcctggtact cctctatatt tatgggaccc attcacataa actttttaag aagctgggaa 181 ttcctgggcc aacccctctg ccttttctgg gaactatttt gttctacctt aggaggtctc 241 tgaataagat tcccagctgg gcgtggtggc tcacgcctgt aatcccagca ctttgggagg 301 ctgaagcagg aggatcacct aaggtcagga gttcgagacc agctttgcca acatgggtct 361 ttggaatttt gacagagaat gtaatgaaaa atacggagaa atgtggggca ctctttccat 421 ttcttacccc agtttttgaa gccctaaata tcggtttgtt tccaaaagat gttacccatt 481 ttttaaaaaa ttccattgaa aggatgaaag aaagtcgcct caaagataaa caaaagcatc 541 gagtagattt ctttcaacag atgatcgact cccagaattc caaagaaaca aagtcccata 601 aagctctgtc tgatctggag cttgtggccc agtcaattat catcattttt gctgcctatg 661 acacaactag caccactctc cccttcatta tgtatgaact ggccactcac cctgatgtcc 721 agcagaaact gcaggaggag attgacgcag ttttacccaa taaggcacct gtcacctacg 781 atgcccTGGT ACAGATGGAG TACCTTGACA TGGTGGTGAA TGAAACGCTC AGATTATTCC 841 CAGTTGTTAG TAGAGTTACG AGAGTCTGCA AGAAAGATAT TGAAATCAAT GGAGTGTTCA 901 TTCCCAAAGG GTTAGCAGTG ATGGTTCCAA TCTATGCTCT TCACCATGAC CCAAAGTACT 961 GGACAGAGCC TGAGAAGTTC TGCCCTGAAA GGTTCAGTAA GAAGAACAAG GACAGCATAG 1021 ATCTTTACAG ATACATACCT TTTGGAGCTG GACCCCGAAA CTGCATTGGC ATGAGGTTTG 1081 CTCTCACAAA CATAAAACTT GCTGTCATTA GAGCACTGCA GAACTTCTCC TTCAAACCTT 1141 GTAAAGAGAC TCAGATCCCA CTGAAATTAG ACAATCTACC AATTCTTCAA CCAGAAAAAC 1201 CTATTGTTCT AAAAGTGCAC TTAAGAGATG GGATTACAAG TGGACCCTTG CCAACTTTCT 1261 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1321 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1381 GTGGAAAGGA CGAGATAGCC AGTTTGCATA GCTTGAAACG CGTTAAGTCg acaatcaacc 1441 tctggattac aaaatttgtg aaagatt