Construct: ORF TRCN0000491834

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004640.2_s317c1
Derived from:
ccsbBroadEn_10461
DNA Barcode:
GGCGCATTCTGATCGATACCCATC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PCDHA6 (56142)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491834
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 56142 PCDHA6 protocadherin alpha 6 NM_018909.4 99.8% 99.8% 1275A>G;1314C>A;2848C>T
2 human 56140 PCDHA8 protocadherin alpha 8 NM_018911.3 92.2% 90.4% (many diffs)
3 human 56142 PCDHA6 protocadherin alpha 6 NM_031848.3 84.2% 84% (many diffs)
4 human 56145 PCDHA3 protocadherin alpha 3 NM_018906.3 83.1% 82.4% (many diffs)
5 human 56143 PCDHA5 protocadherin alpha 5 NM_018908.3 82.4% 81.4% (many diffs)
6 human 56137 PCDHA12 protocadherin alpha 12 NM_018903.4 82.1% 80.7% (many diffs)
7 human 56144 PCDHA4 protocadherin alpha 4 NM_018907.4 82% 81.4% (many diffs)
8 human 56147 PCDHA1 protocadherin alpha 1 NM_018900.3 81.9% 80.8% (many diffs)
9 human 56146 PCDHA2 protocadherin alpha 2 NM_018905.3 81.7% 80.8% (many diffs)
10 human 56138 PCDHA11 protocadherin alpha 11 NM_018902.4 81.6% 81% (many diffs)
11 human 9752 PCDHA9 protocadherin alpha 9 NM_031857.1 80.7% 80.1% (many diffs)
12 human 56141 PCDHA7 protocadherin alpha 7 NM_018910.3 79.7% 80.6% (many diffs)
13 human 56140 PCDHA8 protocadherin alpha 8 NM_031856.1 77.3% 74.1% (many diffs)
14 human 56142 PCDHA6 protocadherin alpha 6 NM_031849.3 72.1% 72.1% (many diffs)
15 human 56139 PCDHA10 protocadherin alpha 10 NM_031860.3 60.3% 58.8% (many diffs)
16 mouse 12937 Pcdha6 protocadherin alpha 6 NM_007767.3 83.4% 85% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2913
ORF length:
2847
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gtttaccccg gaggatagat tgggaaagca atgtctgctc ctcccgcttc
121tgctcctcgc agcctggaag gtggggagcg gccagctcca ctactccgta cccgaggagg
181ccaaacacgg caccttcgtg ggccggatcg cgcaggacct ggggctggag ctggcggagc
241tggtgccgcg cctgttcagg atggcctcca aagaccgcga ggaccttctg gaggtaaatc
301tgcagaatgg cattttgttt gtgaattctc ggatcgaccg cgaggagctg tgcgggcgga
361gcgcggagtg cagcatccac ctggaggtga tcgtggacag gccgctgcag gttttccatg
421tggacgtgga ggtgagggac attaacgaca acccgccctt gttcccggta gaggaacaaa
481gagtgctgat ttacgaatct aggctgccag attctgtgtt tccactggag ggcgcgtccg
541atgcagatgt tggctcaaat tccatcttaa cctataaact cagttctagc gaatacttcg
601ggctagatgt gaaaataaac agtgatgaca ataaacaaat tgggctctta ttaaagaaat
661ccttggacag agaggaagct cctgcacaca acttattcct gacagccaca gatgggggca
721aacctgagct cacaggcact gttcagctgc tggtcacagt gctggatgtg aatgataatg
781ctcccacttt cgaacagtct gaatacgaag taagaatatt cgaaaatgca gacaacggaa
841caacagttat cagactgaat gcttctgatc gggatgaagg agcgaatggg gcaatttcat
901attcttttaa tagccttgtt gcagccatgg ttattgacca ctttagcata gatcgaaata
961cgggagaaat agtgattcgg ggtaatttgg attttgaaca agaaaactta tacaaaatcc
1021tcattgacgc cacggacaaa ggccatcctc ccatggcggg tcattgcacc gttttagtga
1081gaattttgga taaaaatgat aacgtccctg agatagcact gacttcctta tccttgcctg
1141tacgtgaaga cgctcaattt ggtactgtca tcgccctaat tagcgtgaac gacctcgatt
1201caggtgccaa cgggcaggtg aactgctcgc tgacgcctca cgtccctttc aagctggtgt
1261ccaccttcaa gaattactac tcgttggtgc tggacagtgc cctggaccgc gagagcgtgt
1321cggcctatga gttggtggtg accgcgcggg acgggggctc gccttcgctg tgggccacag
1381ccagcttgtc tgtggaggtg gccgacatga atgacaatgc tccggcgttc gcgcagcccg
1441agtacacagt gttcgtgaag gagaacaacc cgccgggctg ccacatcttc acggtgtctg
1501cgcgagacgc ggacgcgcag gagaacgcgc tggtgtccta ctcgctggtg gagcggcggg
1561tgggcgagcg cgcgttgtcg agctacattt cggtgcacgc ggagagcggc aaggtgtacg
1621cgctgcagcc gctggaccac gaggagctag agctgctgca gtttcaggtg agcgcgcgcg
1681acgcgggcgt gccgcctctg ggcagcaacg tgacgctgca ggtgttcgtg ctggacgaga
1741acgacaacgc gccggcgctg ctggcgcctc gggtgggtgg tactggtggt gcagtgagcg
1801agctggtgcc gcggtcactg ggtgcaggcc aagtggtggc gaaggtgcgc gcagttgacg
1861ccgactcagg ctacaacgcg tggctttcgt atgagctgca gcccccggca agcagcgctc
1921gcttcccgtt tcgcgtgggg ctgtacacgg gcgagatcag caccactcgt gtcctggacg
1981aagcggactc tccgcgccac cggctgctgg tgctggtgaa agaccacggt gagccggcgc
2041tgacagcgac ggccacggtt ctggtgtcgc tggtggagag tggccaggct ccaaaggcgt
2101catcacgggc gtcggtgggc gccgcgggcc cagaggcggc gctggtggat gtcaacgtgt
2161acctgatcat cgccatctgc gcggtatcca gcctgctggt cctcacgcta ctgctgtaca
2221cagcgctgcg gtgctcggcg ccacccaccg agggcgcgtg cacggcggac aagcccacgc
2281tggtgtgctc cagcgcagtg gggagctggt cgtactcgca gcagaggcgg cagagggtgt
2341gctccgggga gggcccaccc aagatggatc tcatggcctt tagccccagc ctttcacctt
2401gtcctattat gatgggtaag gcggagaatc aggatttaaa tgaagatcat gatgccaaac
2461cacgacagcc caaccctgac tggcgttact ctgcctccct gagagcaggc atgcacagct
2521ctgtgcacct agaggaggct ggcattcTAC GGGCTGGTCC AGGAGGGCCT GATCAGCAGT
2581GGCCAACAGT ATCCAGTGCA ACACCAGAAC CAGAGGCAGG AGAAGTGTCC CCTCCAGTCG
2641GTGCGGGTGT CAACAGCAAC AGCTGGACCT TTAAATACGG ACCAGGCAAC CCCAAACAAT
2701CCGGTCCCGG TGAGTTGCCC GACAAATTCA TTATCCCAGG ATCTCCTGCA ATCATCTCCA
2761TCCGGCAGGA GCCTACTAAC AGCCAAATTG ACAAAAGTGA CTTCATAACC TTCGGCAAAA
2821AGGAGGAGAC CAAGAAAAAG AAGAAAAAGA AGAAGGGTAA CAAGACCCAG GAGAAAAAAG
2881AGAAAGGGAA CAGCACGACT GACAACAGTG ACTAGAGCCC AACTTTCTTG TACAAAGTGG
2941TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
3001CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
3061AGGCGCATTC TGATCGATAC CCATCACGCG TTAAGTCgac aatcaacctc tggattacaa
3121aatttgtgaa agatt

Download FASTA (ORF) (Full)