Construct: ORF TRCN0000491941

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF020484.2_s317c1
DNA Barcode:
ATCCCACGGCATTCTGCCTCAGCT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
MAST3 (23031)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491941
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23031 MAST3 microtubule associated seri... XM_006722698.2 52.7% 51.5% (many diffs)
2 human 23031 MAST3 microtubule associated seri... XM_017026514.1 52.7% 51.5% (many diffs)
3 human 23031 MAST3 microtubule associated seri... XM_017026515.1 52.7% 51.5% (many diffs)
4 human 23031 MAST3 microtubule associated seri... NM_015016.2 50.1% 48.9% (many diffs)
5 human 23031 MAST3 microtubule associated seri... XM_006722700.3 49.9% 48.7% (many diffs)
6 human 23031 MAST3 microtubule associated seri... XM_005259827.3 49.8% 48.7% (many diffs)
7 human 23031 MAST3 microtubule associated seri... XM_017026513.1 49.8% 48.6% (many diffs)
8 human 23031 MAST3 microtubule associated seri... XM_017026512.1 49.7% 48.6% (many diffs)
9 human 23031 MAST3 microtubule associated seri... XM_005259826.3 49.5% 48.4% (many diffs)
10 human 23031 MAST3 microtubule associated seri... XM_005259825.2 49.5% 48.3% (many diffs)
11 human 23031 MAST3 microtubule associated seri... XM_005259824.2 49.5% 48.3% (many diffs)
12 human 23031 MAST3 microtubule associated seri... XM_005259823.3 49.2% 48.1% (many diffs)
13 human 23031 MAST3 microtubule associated seri... XM_017026511.1 48.7% 47.5% (many diffs)
14 human 23031 MAST3 microtubule associated seri... XM_011527823.1 48.4% 47.2% (many diffs)
15 human 23031 MAST3 microtubule associated seri... XM_017026510.2 46.7% 45.6% (many diffs)
16 human 23031 MAST3 microtubule associated seri... XM_017026516.1 46.5% 45.4% (many diffs)
17 human 23031 MAST3 microtubule associated seri... XM_017026509.1 46.5% 45.4% (many diffs)
18 human 23031 MAST3 microtubule associated seri... XM_017026508.1 46.5% 45.4% (many diffs)
19 human 23031 MAST3 microtubule associated seri... XM_017026507.1 46.4% 45.3% (many diffs)
20 human 23031 MAST3 microtubule associated seri... XM_017026506.1 46.2% 45.1% (many diffs)
21 human 23031 MAST3 microtubule associated seri... XM_006722699.2 46.2% 45.1% (many diffs)
22 mouse 546071 Mast3 microtubule associated seri... XM_011242315.1 46.3% 49.5% (many diffs)
23 mouse 546071 Mast3 microtubule associated seri... XM_006509713.3 42.4% 45.3% (many diffs)
24 mouse 546071 Mast3 microtubule associated seri... NM_199308.2 42.4% 45.3% (many diffs)
25 mouse 546071 Mast3 microtubule associated seri... XM_006509712.3 42.2% 45.1% (many diffs)
26 mouse 546071 Mast3 microtubule associated seri... XM_017312912.1 42.2% 45.1% (many diffs)
27 mouse 546071 Mast3 microtubule associated seri... XM_017312911.1 41.9% 44.7% (many diffs)
28 mouse 546071 Mast3 microtubule associated seri... XM_006509711.2 41.8% 44.7% (many diffs)
29 mouse 546071 Mast3 microtubule associated seri... XM_017312910.1 41.8% 44.7% (many diffs)
30 mouse 546071 Mast3 microtubule associated seri... XM_017312909.1 41.7% 44.5% (many diffs)
31 mouse 546071 Mast3 microtubule associated seri... XM_017312908.1 41.6% 44.4% (many diffs)
32 mouse 546071 Mast3 microtubule associated seri... XM_006509710.2 40.9% 43.7% (many diffs)
33 mouse 546071 Mast3 microtubule associated seri... XM_006509709.2 40.9% 43.7% (many diffs)
34 mouse 546071 Mast3 microtubule associated seri... XM_006509708.2 40.7% 43.5% (many diffs)
35 mouse 546071 Mast3 microtubule associated seri... XM_006509707.2 40.7% 43.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
72
ORF end:
2040
ORF length:
1968
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcttcac catgaatcac gtgtaccggg agaggttccc caaggccaca gcacagatgg
121agggccgtct gcaggagttc ctgacggcct acgcgcccgg cgcccggctg gcgctggctg
181atggcgtctt gggcttcatc caccaccaga tcgtcgagct ggcccgagac tgcttggcca
241agtctggcga gaacctcgtc acctcccgct acttcctaga gatgcaggag aagctggagc
301ggcttctgca ggatgcccat gagcgttcgg acagtgagga ggtcagcttc atcgtccagc
361ttgtccggaa actgctgatc atcatctcac ggccagctcg gctgctggag tgtctggagt
421ttgaccctga ggaattttac cacctgctgg aggcggctga gggccatgcg cgggagggcc
481aaggcattaa gactgacctt ccacagtaca tcattgggca gctgggcctg gccaaggacc
541ccctggagga gatggtgcca ctgagtcacc tcgaagaaga acagccccca gcacctgagt
601ccccagagag ccgcgccctg gtcggccagt cacggaggaa gccatgcgaa agcgactttg
661agaccatcaa actcattagc aacggagcct atggggccgt ctacctggtg cggcaccgtg
721acacacggca gcgctttgcc atcaagaaga tcaacaaaca gaacttgatc ctgcgtaacc
781agatccagca ggtctttgtg gagcgtgaca ttctcacctt tgccgagaac ccctttgtgg
841tcagcatgtt ctgctccttt gagacccggc gccacctatg tatggtcatg gaatacgtgg
901aaggcggcga ctgcgccacg ctcctgaaga acatgggccc gctgcccgtg gacatggccc
961gcctgtactt cgccgagacg gtgttggcgc tggagtacct gcataactat ggcatcgtgc
1021accgtgacct caaaccagac aatctgctca tcacctcgct tggccacatc aagctcacgg
1081acttcggcct gtccaagatc ggcctcatga gcatggccac caacctctat gagggccaca
1141tcgagaagga cgcccgagag ttcatcgaca agcaggtgtg tgggacgccg gagtacatag
1201cccccgaggt gatcttccgc cagggctatg ggaagccagt ggactggtgg gccatgggcg
1261tcgtcctcta tgagtttctg gtgggctgcg tgcctttctt tggagatacc cccgaggaac
1321tcttcggtca ggtggtcagc gatgagatca tgtggccaga gggagatgag gcccttccag
1381cagacgccca ggacctcatc accaggttgc tccggcagag cccgctggac cgtctgggca
1441ctggtggcac ccacgaagtg aagcagcacc cctttttcct ggccctggac tgggcagggc
1501ttctccgaca caaagccgag ttcgtgcccc agctcgaagc cgaggatgat accagctact
1561ttgacacacg ttcggaacgt taccgccatc tgggctccga ggacgacgag accaatgatg
1621aagaatcgtc cacagagatc ccccagttct ccTCCTGCTC CCACCGGTTC AGCAAGGTCT
1681ACAGCAGCTC TGAGTTCCTG GCCGTCCAGC CCACTCCTAC CTTCGCTGAA AGGAGCTTCA
1741GTGAAGACCG GGAGGAGGGG TGGGAGCGCA GCGAAGTGGA CTATGGCCGC CGGCTGAGTG
1801CTGACATCCG GCTGAGGTCC TGGACATCCT CTGGATCCTC CTGTCAGTCA TCTTCGTCCC
1861AGCCCGAGCG GGGTCCCAGC CCATCTCTCC TGAATACCAT CAGCCTGGAC ACAATGCCCA
1921AGTTTGCCTT CTCATCAGAG GATGAGGGGG TAGGCCCAGG CCCTGCAGGC CCCAAGAGGC
1981CACTGGGCTG CCCACCTCAG TGCCACAGAT CGCCGTGGAG GGCGAGGAAG CCGTGCCAGT
2041AGCTCTCGGG CCCACCGGAA GAGACTAGGA CCCAGCTTTC TTGTACAAAG TGGTTGATAT
2101CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC
2161GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATCCCA
2221CGGCATTCTG CCTCAGCTAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt
2281gaaagatt

Download FASTA (ORF) (Full)