Construct: ORF TRCN0000491967
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018500.1_s317c1
- Derived from:
- ccsbBroadEn_09109
- DNA Barcode:
- GGTTATATGCATCTGTGTACGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FERMT3 (83706)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491967
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83706 | FERMT3 | fermitin family member 3 | NM_031471.6 | 99.9% | 100% | 729C>T |
| 2 | human | 83706 | FERMT3 | fermitin family member 3 | XM_017018398.2 | 99.9% | 100% | 729C>T |
| 3 | human | 83706 | FERMT3 | fermitin family member 3 | NM_178443.2 | 99.3% | 99.4% | 729C>T;1079_1090del |
| 4 | human | 83706 | FERMT3 | fermitin family member 3 | XM_011545294.3 | 99.3% | 99.4% | 729C>T;1079_1090del |
| 5 | human | 83706 | FERMT3 | fermitin family member 3 | XM_017018399.1 | 72.8% | 72.8% | 0_1ins540;189C>T |
| 6 | human | 83706 | FERMT3 | fermitin family member 3 | XM_011545295.2 | 72.3% | 72.4% | 0_1ins540;189C>T;539_550del |
| 7 | mouse | 108101 | Fermt3 | fermitin family member 3 | NM_153795.2 | 86.6% | 93.8% | (many diffs) |
| 8 | mouse | 108101 | Fermt3 | fermitin family member 3 | XM_006526593.3 | 86.6% | 93.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2055
- ORF length:
- 1989
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggggatgaag acagcctccg gggactacat cgactcgtca tgggagctgc 121 gggtgtttgt gggagaggag gacccagagg ccgagtcggt caccctgcgg gtcactgggg 181 agtcgcacat cggcggggtg ctcctgaaga ttgtggagca gatcaatcgc aagcaggact 241 ggtcagacca tgctatttgg tgggaacaga agaggcagtg gctgctgcag acccactgga 301 cactggacaa gtacgggatc ctggccgacg cacgcctctt ctttgggccc cagcaccggc 361 ccgtcatcct tcggttgccc aaccgccgcg cactgcgcct ccgtgccagc ttctcccagc 421 ccctcttcca ggctgtggct gccatctgcc gcctcctcag catccggcac cccgaggagc 481 tgtccctgct ccgggctcct gagaagaagg agaagaagaa gaaagagaag gagccagagg 541 aagagctcta tgacttgagc aaggttgtct tggctggggg cgtggcacct gcactgttcc 601 gggggatgcc agctcacttc tcggacagcg cccagactga ggcctgctac cacatgctga 661 gccggcccca gccgccaccc gaccccctcc tgctccagcg tctgccacgg cccagctccc 721 tgtcagacaa gacccagctc cacagcaggt ggctggactc gtcgcggtgt ctcatgcagc 781 agggcatcaa ggctggggac gcactctggc tgcgcttcaa gtactacagc ttcttcgatt 841 tggatcccaa gacagacccc gtgcggctga cacagctgta tgagcaggcc cggtgggacc 901 tgctgctgga ggagattgac tgcaccgagg aggagatgat ggtgtttgcc gccctgcagt 961 accacatcaa caagctgtcc cagagcgggg aggtggggga gccggctggc acagacccag 1021 ggctggacga cctggatgtg gccctgagca acctggaggt gaagctggag gggtcggcgc 1081 ccacagatgt gctggacagc ctcaccacca tcccagagct caaggaccat ctccgaatct 1141 ttcggccccg gaagctgacc ctgaagggct accgccaaca ctgggtggtg ttcaaggaga 1201 ccacactgtc ctactacaag agccaggacg aggcccctgg ggaccccatt cagcagctca 1261 acctcaaggg ctgtgaggtg gttcccgatg ttaacgtctc cggccagaag ttctgcatta 1321 aactcctagt gccctcccct gagggcatga gtgagatcta cctgcggtgc caggatgagc 1381 agcagtatgc ccgctggatg gctggctgcc gcctggcctc caaaggccgc accatggccg 1441 acagcagcta caccagcgag gtgcaggcca tcctggcctt cctcagcctg cagcgcacgg 1501 gcagtggggg cccgggcaac cacccccacg gccctgatgc ctctgccgag ggcctcaacc 1561 cctacggcct cgttgccccc cgtttccagc gaaagttcaa ggccaagcag ctcaccccac 1621 ggatcctgga agcccaccag aatgtggccc agttgtcgct ggcagaggcc cagctgcgct 1681 tcatccaggc ctggcagtcc ctgcccgact tcggcatctc cTATGTCATG GTCAGGTTCA 1741 AGGGCAGCAG GAAAGACGAG ATCCTGGGCA TCGCCAACAA CCGACTGATC CGCATCGACT 1801 TGGCCGTGGG CGACGTGGTC AAGACCTGGC GTTTCAGCAA CATGCGCCAG TGGAATGTCA 1861 ACTGGGACAT CCGGCAGGTG GCCATCGAGT TTGATGAACA CATCAATGTG GCCTTCAGCT 1921 GCGTGTCTGC CAGCTGCCGA ATTGTACACG AGTATATCGG GGGCTACATT TTCCTGTCGA 1981 CGCGGGAGCG GGCCCGTGGG GAGGAGCTGG ATGAAGACCT CTTCCTGCAG CTCACCGGGG 2041 GCCATGAGGC CTTCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 2101 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 2161 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GGTTATATGC ATCTGTGTAC 2221 GGTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt