Construct: ORF TRCN0000492012
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001763.1_s317c1
- Derived from:
- ccsbBroadEn_11117
- DNA Barcode:
- TGATACCACTGCTATCCAACTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SALL2 (6297)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492012
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6297 | SALL2 | spalt like transcription fa... | NR_111967.2 | 33.5% | 1_219del;577T>C;814_1769del | |
| 2 | human | 6297 | SALL2 | spalt like transcription fa... | NR_111966.2 | 31.9% | (many diffs) | |
| 3 | human | 6297 | SALL2 | spalt like transcription fa... | NM_001291447.2 | 19.6% | 15.1% | (many diffs) |
| 4 | human | 6297 | SALL2 | spalt like transcription fa... | NM_001291446.2 | 18.5% | 13.3% | (many diffs) |
| 5 | human | 6297 | SALL2 | spalt like transcription fa... | NM_001364564.1 | 17.8% | 13.5% | (many diffs) |
| 6 | human | 6297 | SALL2 | spalt like transcription fa... | XM_011537065.2 | 17.2% | 13% | (many diffs) |
| 7 | human | 6297 | SALL2 | spalt like transcription fa... | NM_005407.3 | 16.8% | 11.9% | (many diffs) |
| 8 | human | 6297 | SALL2 | spalt like transcription fa... | XM_011537064.1 | 16.2% | 11.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 660
- ORF length:
- 594
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gcacgaatcc gagaggagct ctcgtctcgg ggtgccctgc ggggagccgg 121 cagagctcgg aggtgatgct agcgaggagg atcaccccca agtctgtgcc aagtgctgcg 181 cacaattcac tgacccaact gaattcctcg cccaccagaa cgcatgttct actgaccctc 241 ctgtaatggt gataattggg ggccaggaga accccaacaa ctcttcggcc tcctctgaac 301 cccggccTGA GGGTCACAAT AATCCTCAGG TCATGGACAC AGAGCATAGC AACCCCCCAG 361 ATTCTGGGTC CTCCGTGCCC ACGGATCCCA CCTGGGGCCC AGAGAGGAGA GGAGAGGAGT 421 CTCCAGGGCA TTTCCTGGTC GCTGCCACAG AACCAGTATG TGGCATTCCT GTCAAATGGC 481 CTGCCCATGA AGCCCTGGAA TTCCAGCTCC ACCTCCACTA CCACTCCAAG CCTGGCCCCA 541 CCAGTGCTGT TTGGCCTAGG AACTGTGGCT GGGAAGGTGC CTCCAACAAT GGGATCCAGG 601 GAAGCCAAGG AGAAGACAGC CCCCCTCCTA TTTCAGCCTC CTGCACCCAA GGCAGTGCCT 661 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 721 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 781 TTTATATATC TTGTGGAAAG GACGATGATA CCACTGCTAT CCAACTCAAA CGCGTTAAGT 841 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt