Construct: ORF TRCN0000492013
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013278.1_s317c1
- Derived from:
- ccsbBroadEn_05574
- DNA Barcode:
- GATTTCCCTGGCAGTAGGAAGCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C16orf87 (388272)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492013
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 388272 | C16orf87 | chromosome 16 open reading ... | NM_001001436.4 | 100% | 100% | |
| 2 | human | 388272 | C16orf87 | chromosome 16 open reading ... | XM_024450271.1 | 80.1% | 69.8% | (many diffs) |
| 3 | human | 388272 | C16orf87 | chromosome 16 open reading ... | XM_024450272.1 | 80.1% | 69.8% | (many diffs) |
| 4 | human | 388272 | C16orf87 | chromosome 16 open reading ... | NM_001348660.2 | 60.3% | 60.3% | 161_162ins183 |
| 5 | human | 388272 | C16orf87 | chromosome 16 open reading ... | NM_001348661.2 | 44.7% | 33.5% | (many diffs) |
| 6 | human | 388272 | C16orf87 | chromosome 16 open reading ... | NR_145837.2 | 6.2% | (many diffs) | |
| 7 | human | 388272 | C16orf87 | chromosome 16 open reading ... | NR_145836.2 | 6.2% | (many diffs) | |
| 8 | human | 388272 | C16orf87 | chromosome 16 open reading ... | NR_145835.2 | 6% | (many diffs) | |
| 9 | human | 388272 | C16orf87 | chromosome 16 open reading ... | NR_145834.2 | 4.1% | (many diffs) | |
| 10 | mouse | 66714 | 4921524J17Rik | RIKEN cDNA 4921524J17 gene | NM_025722.3 | 95% | 98.7% | (many diffs) |
| 11 | mouse | 66714 | 4921524J17Rik | RIKEN cDNA 4921524J17 gene | NR_033144.1 | 21.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 528
- ORF length:
- 462
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgcaactcga gccaagaaag tgaagatggC CACCAAATCA TGCCCCGAGT 121 GCGACCAACA GGTTCCTGTT GCATGTAAAT CATGTCCTTG TGGTTACATA TTTATTAGCA 181 GAAAACTTTT AAATGCAAAA CACTCAGAGA AATCACCACC TTCTACAGAA AACAAGCATG 241 AGGCCAAGAG GAGGCGAACA GAGAGAGTTA GGAGAGAGAA GATAAATTCT ACAGTAAATA 301 AAGATTTAGA AAACAGAAAG AGGTCTCGAA GTAACAGCCA TTCAGATCAT ATCAGACGAG 361 GAAGAGGAAG ACCTAAAAGT GCATCTGCCA AAAAACATGA GGAAGAAAGA GAGAAACAGG 421 AAAAGGAAAT TGACATCTAT GCTAACCTGT CTGATGAAAA GGCTTTCGTG TTTTCAGTCG 481 CCTTGGCAGA AATAAATAGA AAAATTATCA ATCAAAGACT TATTCTCTGC CCAACTTTCT 541 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 601 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 661 GTGGAAAGGA CGAGATTTCC CTGGCAGTAG GAAGCGCACG CGTTAAGTCg acaatcaacc 721 tctggattac aaaatttgtg aaagatt