Construct: ORF TRCN0000492052
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000982.1_s317c1
- Derived from:
- ccsbBroadEn_06779
- DNA Barcode:
- CGACCCACTTGCTACGAGTGGATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRODH (5625)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492052
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5625 | PRODH | proline dehydrogenase 1 | NM_001195226.2 | 99.7% | 99.7% | 229T>C;667T>C;1299C>T |
| 2 | human | 102724788 | LOC102724788 | proline dehydrogenase 1, mi... | NM_001368250.1 | 99.5% | 99.5% | (many diffs) |
| 3 | human | 102724788 | LOC102724788 | proline dehydrogenase 1, mi... | XM_017030169.1 | 84.4% | 69.1% | (many diffs) |
| 4 | human | 102724788 | LOC102724788 | proline dehydrogenase 1, mi... | XM_017030167.1 | 83.4% | 68% | (many diffs) |
| 5 | human | 102724788 | LOC102724788 | proline dehydrogenase 1, mi... | XM_006724936.3 | 82.7% | 69.3% | (many diffs) |
| 6 | human | 5625 | PRODH | proline dehydrogenase 1 | NM_016335.5 | 81.8% | 81.8% | (many diffs) |
| 7 | human | 102724788 | LOC102724788 | proline dehydrogenase 1, mi... | NM_001368249.1 | 81.6% | 81.6% | (many diffs) |
| 8 | human | 102724788 | LOC102724788 | proline dehydrogenase 1, mi... | XM_017030170.1 | 78.7% | 78.8% | (many diffs) |
| 9 | human | 102724788 | LOC102724788 | proline dehydrogenase 1, mi... | XM_024452505.1 | 78.7% | 78.8% | (many diffs) |
| 10 | human | 102724788 | LOC102724788 | proline dehydrogenase 1, mi... | XR_951398.2 | 65.8% | (many diffs) | |
| 11 | mouse | 19125 | Prodh | proline dehydrogenase | XR_001781796.1 | 51.4% | (many diffs) | |
| 12 | mouse | 19125 | Prodh | proline dehydrogenase | XR_001781795.1 | 48.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1545
- ORF length:
- 1476
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagatgacc ttctatgggc attttgtagc cggggaggac caggagtcca 121 tccagcccct gcttcggcac tacagggcct tcggtgtcag cgccatcctg gactatggag 181 tggaggagga cctgagcccc gaggaggcag agcacaagga gatggagtcc tgcacctcag 241 ctgcggagag ggatggcagt ggcacgaata agcgggacaa gcaataccag gcccaccggg 301 ccttcgggga ccgcaggaat ggtgtcatca gtgcccgcac ctacttctac gccaatgagg 361 ccaagtgcga cagccacatg gagacattct tgcgctgcat cgaagcctca ggtagagtca 421 gcgatgacgg cttcatagcc attaagctca cagcactggg gagaccccag tttctgctgc 481 agttctcaga ggtgctggcc aagtggaggt gcttctttca ccaaatggct gtggagcaag 541 ggcaggcggg cctggctgcc atggacacca agctggaggt ggcggtgctg caggaaagtg 601 tcgcaaagtt gggcatcgca tccagggctg agattgagga ctggttcacg gcagagaccc 661 tgggagtgtc tggcaccatg gacctgctgg actggagcag cctcatcgac agcaggacca 721 agctgtccaa gcacctggta gtccccaacg cacagacagg acagctggag cccctgctgt 781 cccggttcac tgaggaggag gagctacaga tgaccaggat gctacagcgg atggatgtcc 841 tggccaagaa agccacagag atgggcgtgc ggctgatggt ggatgccgag cagacctact 901 tccagccggc catcagccgc ctgacgctgg agatgcagcg gaagttcaat gtggagaagc 961 cgctcatctt caacacatac cagtgctacc tcaaggatgc ctatgacaat gtgaccctgg 1021 acgtggagct ggctcgccgt gagggctggt gttttggggc caagctggtg cggggcgcat 1081 acctggccca ggagcgagcc cgtgcggcag agatcGGCTA TGAGGACCCC ATCAACCCCA 1141 CGTACGAGGC CACCAACGCC ATGTACCACA GGTGCCTGGA CTACGTGTTG GAGGAGCTGA 1201 AGCACAACGC CAAGGCCAAG GTGATGGTGG CCTCCCACAA TGAGGACACA GTGCGCTTTG 1261 CACTGCGCAG GATGGAGGAG CTGGGCCTGC ATCCTGCTGA CCACCGGGTG TACTTTGGAC 1321 AGCTGCTAGG CATGTGTGAC CAGATCAGCT TCCCGCTGGG CCAGGCTGGC TACCCCGTGT 1381 ACAAGTACGT GCCCTATGGC CCCGTGATGG AGGTGCTGCC CTACTTGTCC CGCCGTGCCC 1441 TGGAGAACAG CAGCCTCATG AAGGGCACCC ATCGGGAGCG GCAGCTGCTG TGGCTGGAGC 1501 TCTTGAGGCG GCTCCGAACT GGCAACCTCT TCCATCGCCC TGCCTTGCCA ACTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA CGACCCACTT GCTACGAGTG GATCACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt