Construct: ORF TRCN0000492170
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021227.2_s317c1
- DNA Barcode:
- GAGTTACTAGGCCCCGACTTGTTA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- ADGRA2 (25960)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492170
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25960 | ADGRA2 | adhesion G protein-coupled ... | NM_032777.10 | 83.7% | 83.7% | 1607_2257del;3339C>G;3660C>T |
| 2 | human | 25960 | ADGRA2 | adhesion G protein-coupled ... | XM_011544481.2 | 82.3% | 82.3% | (many diffs) |
| 3 | human | 25960 | ADGRA2 | adhesion G protein-coupled ... | XM_011544483.2 | 81.4% | 81.5% | (many diffs) |
| 4 | human | 25960 | ADGRA2 | adhesion G protein-coupled ... | XM_011544482.2 | 80.5% | 80.6% | (many diffs) |
| 5 | mouse | 78560 | Adgra2 | adhesion G protein-coupled ... | NM_054044.2 | 70.6% | 73.6% | (many diffs) |
| 6 | mouse | 78560 | Adgra2 | adhesion G protein-coupled ... | XM_006509223.3 | 66.5% | 69.2% | (many diffs) |
| 7 | mouse | 78560 | Adgra2 | adhesion G protein-coupled ... | XM_006509222.3 | 66.5% | 69.2% | (many diffs) |
| 8 | mouse | 78560 | Adgra2 | adhesion G protein-coupled ... | XM_006509224.3 | 55.6% | 41.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 72
- ORF end:
- 3435
- ORF length:
- 3363
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcttcac catgggcgcc gggggacgca ggatgcgggg ggcgcccgcg cgcctgctgc 121 tgccgctgct gccgtggctc ctgctgctcc tggcgcccga ggctcggggc gcgcccggct 181 gcccgctatc catccgcagc tgcaagtgct cgggggagcg gcccaagggg ctgagcggcg 241 gcgtccctgg cccggctcgg cggagggtgg tgtgcagcgg cggggacctc ccggagcctc 301 ccgagcccgg ccttctgcct aacggcaccg ttaccctgct cttgagcaat aacaagatca 361 cggggctccg caatggctcc ttcctgggac tgtcactgct ggagaagctg gacctgagga 421 acaacatcat cagcacagtg cagccgggcg ccttcctggg cctgggggag ctgaagcgtt 481 tagatctctc caacaaccgg attggctgtc tcacctccga gaccttccag ggcctcccca 541 ggcttctccg actaaacata tctggaaaca tcttctccag tctgcaacct ggggtctttg 601 atgagctgcc agcccttaag gttgtggact tgggcaccga gttcctgacc tgtgactgcc 661 acctgcgctg gctgctgccc tgggcccaga atcgctccct gcagctgtcg gaacacacgc 721 tctgtgctta ccccagtgcc ctgcatgctc aggccctggg cagcctccag gaggcccagc 781 tctgctgcga gggggccctg gagctgcaca cacaccacct catcccgtcc ctacgccaag 841 tggtgttcca gggggatcgg ctgcccttcc agtgctctgc cagctacctg ggcaacgaca 901 cccgcatccg ctggtaccac aaccgagccc ctgtggaggg tgatgagcag gcgggcatcc 961 tcctggccga gagcctcatc cacgactgca ccttcatcac cagtgagctg acgctgtctc 1021 acatcggcgt gtgggcctca ggcgagtggg agtgcaccgt gtccatggcc caaggcaacg 1081 ccagcaagaa ggtggagatc gtggtgctgg agacctctgc ctcctactgc cccgccgagc 1141 gtgttgccaa caaccgcggg gacttcaggt ggccccgaac tctggctggc atcacagcct 1201 accagtcctg cctgcagtat cccttcacct cagtgcccct gggcgggggt gccccgggca 1261 cccgagcctc ccgccggtgt gaccgtgccg gccgctggga gccaggggac tactcccact 1321 gtctctacac caacgacatc accagggtgc tgtacacctt cgtgctgatg cccatcaatg 1381 cctccaatgc gctgaccctg gctcaccagc tgcgcgtgta cacagccgag gccgctagct 1441 tttcagacat gatggatgta gtctatgtgg ctcagatgat ccagaaattt ttgggttatg 1501 tcgaccagat caaagagctg gtagaggtga tggtggacat ggccagcaac ctgatgctgg 1561 tggacgagca cctgctgtgg ctggcccagc gcgaggacaa ggcctgcagc cgcatcgtgg 1621 gtgccctgga gcgcattggg ggggccgccc tcagccccca tgcccagcac atctcagtgg 1681 agctgagcgc ctttcccagg gaggtggggg gcgccggggc agggctgcac cccgtggtat 1741 acccctgcac ggccttgctg ctgctctgcc tcttcgccac catcatcacc tacatcctca 1801 accacagctc catccgtgtg tcccggaaag gctggcacat gctgctgaac ttgtgcttcc 1861 acatagccat gacctctgct gtctttgcgg ggggcatcac actcaccaac taccagatgg 1921 tctgccaggc ggtgggcatc accctgcact actcctccct atccacgctg ctctggatgg 1981 gcgtgaaggc gcgagtgctc cataaggagc tcacctggag ggcaccccct ccgcaagaag 2041 gggaccccgc tctgcctact cccagtccta tgctccggtt ctatttgatc gctggaggga 2101 ttccactcat tatctgtggc atcacagctg cagtcaacat ccacaactac cgggaccaca 2161 gcccctactg ctggctggtg tggcgtccaa gccttggcgc cttctacatc cctgtggctt 2221 tgattctgct catcacctgg atctatttcc tgtgcgccgg gctacgctta cggggtcctc 2281 tggcacagaa ccccaaggcg ggcaacagca gggcctccct ggaggcaggg gaggagctga 2341 ggggttccac caggctcagg ggcagcggcc ccctcctgag tgactcaggt tcccttcttg 2401 ctactgggag cgcgcgagtg gggacgcccg ggcccccgga ggatggtgac agcctctatt 2461 ctccgggagt ccagctaggg gcgctggtga ccacgcactt cctgtacttg gccatgtggg 2521 cctgcggggc tctggcagtg tcccagcgct ggctgccccg ggtggtgtgc agctgcttgt 2581 acggggtggc agcctccgcc ctgggcctct tcgtcttcac tcaccactgt gccaggcgga 2641 gggacgtgag agcctcgtgg cgcgcctgct gcccccctgc ctctcccgcg gccccccatg 2701 ccccgccccg ggccctgccc gccgccgcag aggacggttc cccggtgttc ggggaggggc 2761 ccccctccct caagtcctcc ccaagcggca gcagcggcca tccgctggct ctgggcccct 2821 gcaagctcac caacctgcag ctggcccaga gtcaggtgtg cgaggcgggg gcggcggccg 2881 gcggggaagg agagccggag ccggcgggca cccggggaaa cctcgcccac cgccacccca 2941 acaacgtgca ccacgggcgt cgggcgcaca agagccgggc caagggacac cgcgcggggg 3001 aggcctgcgg caagaaccgg ctcaaggccc TGCGCGGGGG CGCGGCGGGG GCGCTGGAGC 3061 TGCTGTCCAG CGAGAGCGGT AGTCTGCACA ACAGCCCCAC CGACAGCTAC CTGGGCAGCA 3121 GCCGCAACAG CCCGGGCGCC GGCCTGCAGC TGGAAGGCGA GCCCATGCTC ACGCCGTCCG 3181 AGGGCAGCGA CACCAGCGCC GCGCCGCTTT CTGAGGCGGG CCGGGCAGGC CAGCGCCGCA 3241 GCGCCAGCCG CGACAGTCTC AAGGGCGGCG GCGCGCTGGA GAAGGAGAGC CATCGCCGCT 3301 CGTACCCGCT CAACGCCGCC AGCCTAAACG GCGCCCCCAA GGGGGGCAAG TACGACGACG 3361 TCACCCTGAT GGGCGCGGAG GTAGCCAGCG GCGGCTGCAT GAAGACCGGA CTCTGGAAGA 3421 GCGAAACTAC CGTCTAAGAC CCAGCTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 3481 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 3541 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGAGTTAC TAGGCCCCGA 3601 CTTGTTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt