Construct: ORF TRCN0000492204
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000003.1_s317c1
- Derived from:
- ccsbBroadEn_15736
- DNA Barcode:
- CCGAACACCCTCTAACTCGGCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATF7 (11016)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492204
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11016 | ATF7 | activating transcription fa... | NM_001206682.1 | 99.4% | 99.1% | 34C>A;129C>T |
| 2 | human | 11016 | ATF7 | activating transcription fa... | NM_001206683.1 | 99.4% | 99.1% | 34C>A;129C>T |
| 3 | human | 11016 | ATF7 | activating transcription fa... | NM_001366562.1 | 99.4% | 99.1% | 34C>A;129C>T |
| 4 | human | 11016 | ATF7 | activating transcription fa... | NM_001366563.1 | 70.6% | 60.3% | 1_7delATGAAAT;41C>A;52_53ins97 |
| 5 | human | 114108587 | ATF7-NPFF | ATF7-NPFF readthrough | NM_001366560.1 | 23.4% | 18.3% | (many diffs) |
| 6 | human | 114108587 | ATF7-NPFF | ATF7-NPFF readthrough | NM_001366559.1 | 23.2% | 18.2% | (many diffs) |
| 7 | human | 11016 | ATF7 | activating transcription fa... | NM_001130060.2 | 22.5% | 20.1% | (many diffs) |
| 8 | human | 11016 | ATF7 | activating transcription fa... | NM_001366556.2 | 22.3% | 17.5% | (many diffs) |
| 9 | human | 11016 | ATF7 | activating transcription fa... | NM_001366558.2 | 22.3% | 17.5% | (many diffs) |
| 10 | human | 11016 | ATF7 | activating transcription fa... | NM_006856.3 | 22.3% | 17.5% | (many diffs) |
| 11 | human | 11016 | ATF7 | activating transcription fa... | NM_001366555.2 | 20.4% | 19.4% | (many diffs) |
| 12 | human | 11016 | ATF7 | activating transcription fa... | NR_073163.2 | 16% | (many diffs) | |
| 13 | human | 114108587 | ATF7-NPFF | ATF7-NPFF readthrough | NR_159377.1 | 14.5% | (many diffs) | |
| 14 | mouse | 223922 | Atf7 | activating transcription fa... | NM_001310070.1 | 24.7% | 18.9% | (many diffs) |
| 15 | mouse | 223922 | Atf7 | activating transcription fa... | NM_001310066.1 | 21.6% | 16.7% | (many diffs) |
| 16 | mouse | 223922 | Atf7 | activating transcription fa... | NM_001310067.1 | 21.6% | 16.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 417
- ORF length:
- 351
- Sequence:
-
1 tcttcCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGG AGACGACAGA CCGTTTGTGT GCAATGCCAC GGGCTGTGGA CAGAGATTTA 121 CAAACGAGGA CCACCTGGCA GTTCATAAAC ACAAGCATGA GATGACATTG AAATTTGGCC 181 CAGCCCGAAC TGATTCAGTC ATCATTGCAG ATCAAACGCC TACTCCAACT AGATTCCTGA 241 AGAACTGTGA GGAGGTGGGA CTCTTCAATG AACTAGCTAG CTCCTTTGAA CATGAATTCA 301 AGAAAGCTGC AGATGAGGAT GAGAAAAAGG CAAGAAGCAG GACTGTTGCC AAAAAACTGG 361 TGGTATTCAG ACCTAGGCTA TTTTTATTGT GCTTTGGGAT AATTTTCTTA ATTGGTTACC 421 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 481 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 541 ATATATCTTG TGGAAAGGAC GACCGAACAC CCTCTAACTC GGCCTCACGC GTTAAGTCga 601 caatcaacct ctggattaca aaatttgtga aagatt