Construct: ORF TRCN0000492225

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021537.2_s317c1
DNA Barcode:
TATACGCCTCCTCACCATAAATTC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
USP32 (84669)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000492225
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84669 USP32 ubiquitin specific peptidas... NM_032582.4 99.9% 100% 3042C>T
2 human 84669 USP32 ubiquitin specific peptidas... XM_011525376.1 99.2% 98.5% (many diffs)
3 human 84669 USP32 ubiquitin specific peptidas... XM_011525373.1 99.1% 99.1% 1867_1908del;3084C>T
4 human 84669 USP32 ubiquitin specific peptidas... XM_011525372.1 98.4% 98% (many diffs)
5 human 84669 USP32 ubiquitin specific peptidas... XM_011525374.1 98.3% 97.7% (many diffs)
6 human 84669 USP32 ubiquitin specific peptidas... XM_011525375.1 98.2% 98.1% (many diffs)
7 human 84669 USP32 ubiquitin specific peptidas... XM_011525371.1 97.5% 97.1% (many diffs)
8 human 84669 USP32 ubiquitin specific peptidas... XM_017025233.1 96.8% 95.8% (many diffs)
9 human 84669 USP32 ubiquitin specific peptidas... XM_011525378.1 95.9% 95% (many diffs)
10 human 84669 USP32 ubiquitin specific peptidas... XM_011525379.3 81.1% 81.1% 0_1ins873;994_1035del;2211C>T
11 human 84669 USP32 ubiquitin specific peptidas... XM_017025234.1 78.7% 78.7% 0_1ins990;877_918del;2094C>T
12 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524057.2 69.5% 57.9% (many diffs)
13 human 9098 USP6 ubiquitin specific peptidase 6 NM_001304284.2 69.1% 58.4% (many diffs)
14 human 9098 USP6 ubiquitin specific peptidase 6 NM_004505.4 69.1% 58.4% (many diffs)
15 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524050.1 69.1% 58.4% (many diffs)
16 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524051.2 69.1% 58.4% (many diffs)
17 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524052.2 69.1% 58.4% (many diffs)
18 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524053.2 69.1% 58.4% (many diffs)
19 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524054.2 69.1% 58.4% (many diffs)
20 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524055.2 69.1% 58.4% (many diffs)
21 human 9098 USP6 ubiquitin specific peptidase 6 XM_011524056.2 69.1% 58.4% (many diffs)
22 human 9098 USP6 ubiquitin specific peptidase 6 XM_017025290.1 69.1% 58.4% (many diffs)
23 human 162632 USP32P1 ubiquitin specific peptidas... NR_003190.2 21.4% (many diffs)
24 mouse 237898 Usp32 ubiquitin specific peptidas... NM_001029934.1 91% 95.7% (many diffs)
25 mouse 237898 Usp32 ubiquitin specific peptidas... XM_011248990.1 90.2% 94.9% (many diffs)
26 mouse 237898 Usp32 ubiquitin specific peptidas... XM_011248991.1 50.6% 53% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4881
ORF length:
4812
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61caggcaccat gggtgccaag gagtcacgga tcggattcct cagctacgag gaggcgctga
121ggagagttac agatgtagag ctaaaacgac tgaaggatgc tttcaagagg acctgtggac
181tctcatatta catgggccag cactgcttca tccgggaagt gcttggggat ggagtgcctc
241caaaggttgc tgaggtgatt tactgttctt ttggtggaac atccaaaggg ctgcacttca
301ataatttaat agttggactt gtcctcctta caagaggcaa agatgaagag aaagcaaaat
361acatttttag tcttttttca agtgaatctg ggaactatgt tatacgggaa gaaatggaaa
421gaatgctcca cgtggtggat ggtaaagtcc cagatacact caggaagtgt ttctcagagg
481gtgaaaaggt aaactatgaa aagtttagaa attggctttt tctaaacaaa gatgctttta
541ctttctctcg atggcttcta tctggaggtg tgtatgttac cctcactgat gatagtgata
601ctcctacttt ctaccaaact ctggctggag tcacacattt ggaggaatca gacatcattg
661atcttgagaa acgctattgg ttattgaagg ctcaatcccg gactggacga tttgatttag
721agacatttgg cccattggtt tcaccaccta ttcgtccatc tctaagtgaa ggtttgttta
781atgcttttga tgaaaatcgt gacaatcaca tagattttaa ggagatatcc tgtgggttat
841cagcctgttg caggggaccc ctggctgaaa gacaaaaatt ttgcttcaag gtatttgatg
901ttgaccgtga tggagttctc tccagggttg aactgagaga catggtggtt gcacttttag
961aagtctggaa ggacaaccgc actgatgata ttcctgaatt acatatggat ctctctgata
1021ttgtagaagg catactgaat gcacatgaca ccacaaagat gggtcatctt actctggaag
1081actatcagat ctggagtgtg aaaaatgttc ttgccaatga gtttttgaac ctccttttcc
1141aggtgtgtca catagttctg gggttaagac cagctactcc ggaagaagaa ggacaaatta
1201ttagaggatg gttagaacga gagagcaggt atggtctgca agcaggacac aactggttta
1261tcatctccat gcagtggtgg caacagtgga aagaatatgt caaatacgat gccaaccctg
1321tggtaattga gccatcatct gttttgaatg gaggaaaata ctcatttgga actgcagccc
1381atcctatgga gcaggtcgaa gatagaattg gaagcagcct cagttacgtg aatactacag
1441aagagaaatt ttcagacaac atttctactg catctgaagc ctcagaaact gctggcagcg
1501gctttctgta ttctgccaca ccaggggcag atgtttgctt tgctcgacaa cataacactt
1561ctgacaataa caaccagtgt ttgctgggag ccaatgggaa tattttgttg caccttaacc
1621ctcagaaacc aggggctatt gataatcagc cattagtaac tcaagaacca gtaaaggcta
1681catcattaac actagaagga ggacgattaa aacgaactcc acagctgatt catggaagag
1741actatgaaat ggtcccagaa cctgtgtgga gagcacttta tcactggtat ggagcaaacc
1801tggccttacc tagaccagtt atcaagaaca gcaagacaga catcccagag ctggaattat
1861ttccccgcta tcttctcttc ctgagacagc agcctgccac tcggacacag cagtctaaca
1921tctgggtgaa tatgggaaat gtaccttctc cgaatgcacc tttaaagcgg gtattagcct
1981atacaggctg ttttagtcga atgcagacca tcaaggaaat tcacgaatat ctatctcaaa
2041ggctgcgcat taaagaggaa gatatgcgcc tgtggctata caacagtgag aactacctta
2101ctcttctgga tgatgaggat cataaattgg aatatttgaa aatccaggat gaacaacacc
2161tggtaattga agttcgcaac aaagatatga gttggcctga ggagatgtct tttatagcaa
2221atagtagtaa aatagataga cacaaggttc ccacagaaaa gggagccaca ggtctaagca
2281atctgggaaa cacatgcttc atgaactcaa gcatccagtg tgttagtaac acacagccac
2341tgacacagta ttttatctca gggagacatc tttatgaact caacaggaca aatcccattg
2401gtatgaaggg gcatatggct aaatgctatg gtgatttagt gcaggaactt tggagtggaa
2461ctcagaagaa tgttgcccca ttaaagcttc ggtggaccat agcaaaatat gctcccaggt
2521ttaatgggtt tcagcaacag gactcccaag aacttctggc ttttctcttg gatggtcttc
2581atgaagatct taatcgagtc catgaaaagc catatgtgga actgaaggac agtgatgggc
2641gaccagactg ggaagtagct gcagaggcct gggacaacca tctaagaaga aatagatcaa
2701ttgttgtgga tttgttccat gggcagctaa gatctcaagt aaaatgcaag acatgtgggc
2761atataagtgt ccgatttgac cctttcaatt ttttgtcttt gccactacca atggacagtt
2821atatgcactt agaaataaca gtgattaagt tagatggtac tacccctgta cggtatggac
2881taagactgaa tatggatgaa aagtacacag gtttaaaaaa acagctgagt gatctctgtg
2941gacttaattc agaacaaatc cttctagcag aagtacatgg ttccaacata aagaactttc
3001ctcaggacaa ccaaaaagta cgactctcag tgagtggatt tttgtgtgca tttgaaattc
3061ctgtccctgt gtctccaatt tcagcttcta gtccaacaca gacagatttt tcctcttcgc
3121catctacaaa tgaaatgttc accctaacta ccaatgggga cctaccccga ccaatattca
3181tccccaatgg aatgccaaac actgttgtgc catgtggaac tgagaagaac ttcacaaatg
3241gaatggttaa tggtcacatg ccatctcttc ctgacagccc ctttacaggt tacatcattg
3301cagtccaccg aaaaatgatg aggacagaac tgtatttcct gtcatctcag aagaatcgcc
3361ccagcctctt tggaatgcca ttgattgttc catgtactgt gcatacccgg aagaaagacc
3421tatatgatgc ggtttggatt caagtatccc ggttagcgag cccactccca cctcaggaag
3481ctagtaatca tgcccaggat tgtgacgaca gtatgggcta tcaatatcca ttcactctac
3541gagttgtgca gaaagatggg aactcctgtg cttggtgccc atggtataga ttttgcagag
3601gctgtaaaat tgattgtggg gaagacagag ctttcattgg aaatgcctat atcgctgtgg
3661attgggatcc cacagccctt caccttcgct atcaaacatc ccaggaaagg gttgtagatg
3721agcatgagag tgtggagcag agtcggcgag cgcaagccga gcccatcaac ctggacagct
3781gtctccgtgc tttcaccagt gaggaagagc taggggaaaa tgagatgtac tactgttcca
3841agtgtaagac ccactgctta gcaacaaaga agctggatct ctggaggctt ccacccatcc
3901tgattattca ccttaagcga tttcaatttg taaatggtcg gtggataaaa tcacagaaaa
3961ttgtcaaatt tcctcgggaa agttttgatc caagtgcttt tttggtacca agagacccgg
4021ctctctgcca gcataaacca ctcacacccc agggggatga gctctctgag cccaggattc
4081tggcaaggga ggtgaagaaa gtggatgcgc agagttcggc tggggaagag gacgtgctcc
4141tgagcaaaag cccatcctca ctcagcgcta acatcatcag cagcccgaaa ggttctcctt
4201cttcatcaag aaaaagtgga accagctgtc cctccagcaa aaacagcagc cctaatagca
4261gcccacggac tttggggagg agcaaaggga ggctccggct gccccagatt ggcagcaaaa
4321ataaactgtc aagtagtaaa gagaacttgg atgccagcaa agaaaatggg gctgggcaga
4381tatgtgagct ggctgacgcc ttgagtcgag ggcatgtgct ggggggcagc caaccagagt
4441tggtcactcc tcaggaccat gaggtagctt tggccaatgg attcctttat gagcatgaag
4501catgtGGCAA TGGCTACAGC AATGGTCAGC TTGGAAACCA CAGTGAAGAA GACAGCACTG
4561ATGACCAAAG AGAAGATACT CGTATTAAGC CTATTTATAA TCTATATGCA ATTTCGTGCC
4621ATTCAGGAAT TCTGGGTGGG GGCCATTACG TCACTTATGC CAAAAACCCA AACTGCAAGT
4681GGTACTGTTA CAATGACAGC AGCTGTAAGG AACTTCACCC GGATGAAATT GACACCGACT
4741CTGCCTACAT TCTTTTCTAT GAGCAGCAGG GGATAGACTA TGCACAATTT CTGCCAAAGA
4801CTGATGGCAA AAAGATGGCA GACACAAGCA GTATGGATGA AGACTTTGAG TCTGATTACA
4861AAAAGTACTG TGTGTTACAG TAAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA
4921AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
4981TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT ATACGCCTCC
5041TCACCATAAA TTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
5101att

Download FASTA (ORF) (Full)