Construct: ORF TRCN0000492225
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021537.2_s317c1
- DNA Barcode:
- TATACGCCTCCTCACCATAAATTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- USP32 (84669)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492225
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84669 | USP32 | ubiquitin specific peptidas... | NM_032582.4 | 99.9% | 100% | 3042C>T |
| 2 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525376.1 | 99.2% | 98.5% | (many diffs) |
| 3 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525373.1 | 99.1% | 99.1% | 1867_1908del;3084C>T |
| 4 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525372.1 | 98.4% | 98% | (many diffs) |
| 5 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525374.1 | 98.3% | 97.7% | (many diffs) |
| 6 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525375.1 | 98.2% | 98.1% | (many diffs) |
| 7 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525371.1 | 97.5% | 97.1% | (many diffs) |
| 8 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_017025233.1 | 96.8% | 95.8% | (many diffs) |
| 9 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525378.1 | 95.9% | 95% | (many diffs) |
| 10 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_011525379.3 | 81.1% | 81.1% | 0_1ins873;994_1035del;2211C>T |
| 11 | human | 84669 | USP32 | ubiquitin specific peptidas... | XM_017025234.1 | 78.7% | 78.7% | 0_1ins990;877_918del;2094C>T |
| 12 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524057.2 | 69.5% | 57.9% | (many diffs) |
| 13 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | NM_001304284.2 | 69.1% | 58.4% | (many diffs) |
| 14 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | NM_004505.4 | 69.1% | 58.4% | (many diffs) |
| 15 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524050.1 | 69.1% | 58.4% | (many diffs) |
| 16 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524051.2 | 69.1% | 58.4% | (many diffs) |
| 17 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524052.2 | 69.1% | 58.4% | (many diffs) |
| 18 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524053.2 | 69.1% | 58.4% | (many diffs) |
| 19 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524054.2 | 69.1% | 58.4% | (many diffs) |
| 20 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524055.2 | 69.1% | 58.4% | (many diffs) |
| 21 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_011524056.2 | 69.1% | 58.4% | (many diffs) |
| 22 | human | 9098 | USP6 | ubiquitin specific peptidase 6 | XM_017025290.1 | 69.1% | 58.4% | (many diffs) |
| 23 | human | 162632 | USP32P1 | ubiquitin specific peptidas... | NR_003190.2 | 21.4% | (many diffs) | |
| 24 | mouse | 237898 | Usp32 | ubiquitin specific peptidas... | NM_001029934.1 | 91% | 95.7% | (many diffs) |
| 25 | mouse | 237898 | Usp32 | ubiquitin specific peptidas... | XM_011248990.1 | 90.2% | 94.9% | (many diffs) |
| 26 | mouse | 237898 | Usp32 | ubiquitin specific peptidas... | XM_011248991.1 | 50.6% | 53% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4881
- ORF length:
- 4812
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 caggcaccat gggtgccaag gagtcacgga tcggattcct cagctacgag gaggcgctga 121 ggagagttac agatgtagag ctaaaacgac tgaaggatgc tttcaagagg acctgtggac 181 tctcatatta catgggccag cactgcttca tccgggaagt gcttggggat ggagtgcctc 241 caaaggttgc tgaggtgatt tactgttctt ttggtggaac atccaaaggg ctgcacttca 301 ataatttaat agttggactt gtcctcctta caagaggcaa agatgaagag aaagcaaaat 361 acatttttag tcttttttca agtgaatctg ggaactatgt tatacgggaa gaaatggaaa 421 gaatgctcca cgtggtggat ggtaaagtcc cagatacact caggaagtgt ttctcagagg 481 gtgaaaaggt aaactatgaa aagtttagaa attggctttt tctaaacaaa gatgctttta 541 ctttctctcg atggcttcta tctggaggtg tgtatgttac cctcactgat gatagtgata 601 ctcctacttt ctaccaaact ctggctggag tcacacattt ggaggaatca gacatcattg 661 atcttgagaa acgctattgg ttattgaagg ctcaatcccg gactggacga tttgatttag 721 agacatttgg cccattggtt tcaccaccta ttcgtccatc tctaagtgaa ggtttgttta 781 atgcttttga tgaaaatcgt gacaatcaca tagattttaa ggagatatcc tgtgggttat 841 cagcctgttg caggggaccc ctggctgaaa gacaaaaatt ttgcttcaag gtatttgatg 901 ttgaccgtga tggagttctc tccagggttg aactgagaga catggtggtt gcacttttag 961 aagtctggaa ggacaaccgc actgatgata ttcctgaatt acatatggat ctctctgata 1021 ttgtagaagg catactgaat gcacatgaca ccacaaagat gggtcatctt actctggaag 1081 actatcagat ctggagtgtg aaaaatgttc ttgccaatga gtttttgaac ctccttttcc 1141 aggtgtgtca catagttctg gggttaagac cagctactcc ggaagaagaa ggacaaatta 1201 ttagaggatg gttagaacga gagagcaggt atggtctgca agcaggacac aactggttta 1261 tcatctccat gcagtggtgg caacagtgga aagaatatgt caaatacgat gccaaccctg 1321 tggtaattga gccatcatct gttttgaatg gaggaaaata ctcatttgga actgcagccc 1381 atcctatgga gcaggtcgaa gatagaattg gaagcagcct cagttacgtg aatactacag 1441 aagagaaatt ttcagacaac atttctactg catctgaagc ctcagaaact gctggcagcg 1501 gctttctgta ttctgccaca ccaggggcag atgtttgctt tgctcgacaa cataacactt 1561 ctgacaataa caaccagtgt ttgctgggag ccaatgggaa tattttgttg caccttaacc 1621 ctcagaaacc aggggctatt gataatcagc cattagtaac tcaagaacca gtaaaggcta 1681 catcattaac actagaagga ggacgattaa aacgaactcc acagctgatt catggaagag 1741 actatgaaat ggtcccagaa cctgtgtgga gagcacttta tcactggtat ggagcaaacc 1801 tggccttacc tagaccagtt atcaagaaca gcaagacaga catcccagag ctggaattat 1861 ttccccgcta tcttctcttc ctgagacagc agcctgccac tcggacacag cagtctaaca 1921 tctgggtgaa tatgggaaat gtaccttctc cgaatgcacc tttaaagcgg gtattagcct 1981 atacaggctg ttttagtcga atgcagacca tcaaggaaat tcacgaatat ctatctcaaa 2041 ggctgcgcat taaagaggaa gatatgcgcc tgtggctata caacagtgag aactacctta 2101 ctcttctgga tgatgaggat cataaattgg aatatttgaa aatccaggat gaacaacacc 2161 tggtaattga agttcgcaac aaagatatga gttggcctga ggagatgtct tttatagcaa 2221 atagtagtaa aatagataga cacaaggttc ccacagaaaa gggagccaca ggtctaagca 2281 atctgggaaa cacatgcttc atgaactcaa gcatccagtg tgttagtaac acacagccac 2341 tgacacagta ttttatctca gggagacatc tttatgaact caacaggaca aatcccattg 2401 gtatgaaggg gcatatggct aaatgctatg gtgatttagt gcaggaactt tggagtggaa 2461 ctcagaagaa tgttgcccca ttaaagcttc ggtggaccat agcaaaatat gctcccaggt 2521 ttaatgggtt tcagcaacag gactcccaag aacttctggc ttttctcttg gatggtcttc 2581 atgaagatct taatcgagtc catgaaaagc catatgtgga actgaaggac agtgatgggc 2641 gaccagactg ggaagtagct gcagaggcct gggacaacca tctaagaaga aatagatcaa 2701 ttgttgtgga tttgttccat gggcagctaa gatctcaagt aaaatgcaag acatgtgggc 2761 atataagtgt ccgatttgac cctttcaatt ttttgtcttt gccactacca atggacagtt 2821 atatgcactt agaaataaca gtgattaagt tagatggtac tacccctgta cggtatggac 2881 taagactgaa tatggatgaa aagtacacag gtttaaaaaa acagctgagt gatctctgtg 2941 gacttaattc agaacaaatc cttctagcag aagtacatgg ttccaacata aagaactttc 3001 ctcaggacaa ccaaaaagta cgactctcag tgagtggatt tttgtgtgca tttgaaattc 3061 ctgtccctgt gtctccaatt tcagcttcta gtccaacaca gacagatttt tcctcttcgc 3121 catctacaaa tgaaatgttc accctaacta ccaatgggga cctaccccga ccaatattca 3181 tccccaatgg aatgccaaac actgttgtgc catgtggaac tgagaagaac ttcacaaatg 3241 gaatggttaa tggtcacatg ccatctcttc ctgacagccc ctttacaggt tacatcattg 3301 cagtccaccg aaaaatgatg aggacagaac tgtatttcct gtcatctcag aagaatcgcc 3361 ccagcctctt tggaatgcca ttgattgttc catgtactgt gcatacccgg aagaaagacc 3421 tatatgatgc ggtttggatt caagtatccc ggttagcgag cccactccca cctcaggaag 3481 ctagtaatca tgcccaggat tgtgacgaca gtatgggcta tcaatatcca ttcactctac 3541 gagttgtgca gaaagatggg aactcctgtg cttggtgccc atggtataga ttttgcagag 3601 gctgtaaaat tgattgtggg gaagacagag ctttcattgg aaatgcctat atcgctgtgg 3661 attgggatcc cacagccctt caccttcgct atcaaacatc ccaggaaagg gttgtagatg 3721 agcatgagag tgtggagcag agtcggcgag cgcaagccga gcccatcaac ctggacagct 3781 gtctccgtgc tttcaccagt gaggaagagc taggggaaaa tgagatgtac tactgttcca 3841 agtgtaagac ccactgctta gcaacaaaga agctggatct ctggaggctt ccacccatcc 3901 tgattattca ccttaagcga tttcaatttg taaatggtcg gtggataaaa tcacagaaaa 3961 ttgtcaaatt tcctcgggaa agttttgatc caagtgcttt tttggtacca agagacccgg 4021 ctctctgcca gcataaacca ctcacacccc agggggatga gctctctgag cccaggattc 4081 tggcaaggga ggtgaagaaa gtggatgcgc agagttcggc tggggaagag gacgtgctcc 4141 tgagcaaaag cccatcctca ctcagcgcta acatcatcag cagcccgaaa ggttctcctt 4201 cttcatcaag aaaaagtgga accagctgtc cctccagcaa aaacagcagc cctaatagca 4261 gcccacggac tttggggagg agcaaaggga ggctccggct gccccagatt ggcagcaaaa 4321 ataaactgtc aagtagtaaa gagaacttgg atgccagcaa agaaaatggg gctgggcaga 4381 tatgtgagct ggctgacgcc ttgagtcgag ggcatgtgct ggggggcagc caaccagagt 4441 tggtcactcc tcaggaccat gaggtagctt tggccaatgg attcctttat gagcatgaag 4501 catgtGGCAA TGGCTACAGC AATGGTCAGC TTGGAAACCA CAGTGAAGAA GACAGCACTG 4561 ATGACCAAAG AGAAGATACT CGTATTAAGC CTATTTATAA TCTATATGCA ATTTCGTGCC 4621 ATTCAGGAAT TCTGGGTGGG GGCCATTACG TCACTTATGC CAAAAACCCA AACTGCAAGT 4681 GGTACTGTTA CAATGACAGC AGCTGTAAGG AACTTCACCC GGATGAAATT GACACCGACT 4741 CTGCCTACAT TCTTTTCTAT GAGCAGCAGG GGATAGACTA TGCACAATTT CTGCCAAAGA 4801 CTGATGGCAA AAAGATGGCA GACACAAGCA GTATGGATGA AGACTTTGAG TCTGATTACA 4861 AAAAGTACTG TGTGTTACAG TAAGACCCAG CTTTCTTGTA CAAAGTGGTT GATATCGGTA 4921 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 4981 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT ATACGCCTCC 5041 TCACCATAAA TTCACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 5101 att