Construct: ORF TRCN0000492295
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011164.1_s317c1
- Derived from:
- ccsbBroadEn_05228
- DNA Barcode:
- CACGAGTGGCAGCAAAAACTGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCNY (219771)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492295
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 219771 | CCNY | cyclin Y | NM_001282853.1 | 100% | 100% | |
| 2 | human | 219771 | CCNY | cyclin Y | NM_181698.4 | 100% | 100% | |
| 3 | human | 219771 | CCNY | cyclin Y | NM_001282852.1 | 87.8% | 85.4% | (many diffs) |
| 4 | human | 219771 | CCNY | cyclin Y | NM_145012.6 | 84.1% | 84.1% | 1_162del |
| 5 | human | 219771 | CCNY | cyclin Y | NM_001282854.2 | 72.3% | 72.1% | 0_1ins237;2T>G |
| 6 | mouse | 67974 | Ccny | cyclin Y | NM_026484.3 | 77% | 82.4% | (many diffs) |
| 7 | mouse | 67974 | Ccny | cyclin Y | XM_017317975.1 | 72.7% | 76.4% | (many diffs) |
| 8 | mouse | 67974 | Ccny | cyclin Y | XM_017317974.1 | 64.8% | 67.8% | (many diffs) |
| 9 | mouse | 67974 | Ccny | cyclin Y | XM_017317976.1 | 51.7% | 53.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 930
- ORF length:
- 861
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaattcaat ccttcagatc atcctcgggc cagcacaata ttcctcagta 121 aatctcagac ggacgtgaga gaaaaacgca agagtctctt cattaaccat catcctccag 181 gacaaatagc aaggaaatac agttcctgct ccaccatttt cctagatgat agcacagtca 241 gtcaaccaaa cctcaagtat acaattaaat gtgtcgctct tgcaatatat tatcacatca 301 aaaacaggga cccagatgga aggatgctct tagatatttt tgatgaaaat cttcaccctc 361 tttcgaaatc cgaagtgcca ccagattatg acaaacacaa cccagagcag aagcagattt 421 accggttcgt tcggacactg ttcagtgctg ctcagctgac ggctgaatgt gccatcgtca 481 ccctggtgta ccttgaaaga cttttaacat acgcagagat agatatctgt ccggccaact 541 GGAAGCGGAT TGTTTTAGGG GCGATCCTGC TGGCCTCCAA GGTGTGGGAT GACCAGGCTG 601 TATGGAATGT GGATTACTGC CAGATCCTGA AAGACATCAC GGTGGAGGAC ATGAACGAGC 661 TAGAGCGACA GTTTCTTGAA TTGCTGCAGT TCAACATCAA TGTTCCTTCC AGTGTCTATG 721 CCAAGTATTA TTTTGATCTT CGTTCTCTGG CAGAAGCGAA CAACCTGAGC TTTCCCTTGG 781 AGCCCCTGAG CAGGGAGAGG GCTCACAAGC TTGAGGCCAT CTCTCGCCTC TGCGAGGACA 841 AGTACAAGGA CCTAAGAAGA TCCGCGAGGA AGCGCTCAGC CAGTGCAGAC AACCTGACTC 901 TGCCCCGGTG GTCCCCAGCC ATCATCTCTT TGCCAACTTT CTTGTACAAA GTGGTTGATA 961 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 1021 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGACACGA 1081 GTGGCAGCAA AAACTGTGAA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1141 tgaaagatt