Construct: ORF TRCN0000492301
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015151.2_s317c1
- Derived from:
- ccsbBroadEn_13478
- DNA Barcode:
- AGGTTAACTCATATGTTAACTCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FRMD3 (257019)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492301
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_174938.6 | 97% | 96.8% | (many diffs) |
| 2 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_017014588.1 | 95.2% | 93.1% | (many diffs) |
| 3 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_001244960.1 | 94.8% | 93.9% | (many diffs) |
| 4 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_001244959.2 | 90.2% | 89.2% | (many diffs) |
| 5 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_024447487.1 | 86% | 85.7% | (many diffs) |
| 6 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_024447488.1 | 86% | 85.7% | (many diffs) |
| 7 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_024447489.1 | 86% | 85.7% | (many diffs) |
| 8 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_017014589.1 | 69.1% | 68.5% | (many diffs) |
| 9 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_001244961.1 | 62% | 60.7% | (many diffs) |
| 10 | human | 257019 | FRMD3 | FERM domain containing 3 | XM_017014591.2 | 40% | 35.9% | (many diffs) |
| 11 | human | 257019 | FRMD3 | FERM domain containing 3 | NM_001244962.2 | 33.1% | 28.3% | (many diffs) |
| 12 | mouse | 242506 | Frmd3 | FERM domain containing 3 | NM_172869.4 | 86.7% | 88.2% | (many diffs) |
| 13 | mouse | 242506 | Frmd3 | FERM domain containing 3 | NM_001163732.1 | 80.3% | 81.2% | (many diffs) |
| 14 | mouse | 242506 | Frmd3 | FERM domain containing 3 | XM_006537928.2 | 52.5% | 52.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1809
- ORF length:
- 1743
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ccactttcgg agctccagcg tcaaatcgct cagccaggag atgagatgca 121 ccatccggct gctggacgac tcggagatct cctgccacat ccagagggaa accaaagggc 181 agtttctcat tgaccacatc tgcaactact acagcctgct ggagaaggac tactttggca 241 ttcgctatgt ggacccagag aagcaaaggc actggcttga acctaacaag tccatcttca 301 agcaaatgaa aactcatcca ccatacacca tgtgctttag agtgaaattc tacccacatg 361 aacccttgaa gattaaagaa gagctcacaa gatacctttt ataccttcag attaaaaggg 421 acatttttca tggccacctg ctgtgctcct tttctgatgc tgcctacctg ggtgcctgta 481 ttgttcaagc tgagcttggt gattacgatc ctaatgagca tcctgagaat tacatcagtg 541 agtttgagat tttccccaag cagtcacaga agctggaaag aaaaatagtg gaaattcata 601 aaaatgaact cagggggcag agcccaccag ttgctgaatt taacttgctc ctgaaagctc 661 acactttgga aacctacggg gtggatcctc acccatgcaa ggattcaaca ggcacaacaa 721 catttttagg attcacagct gcaggctttg tggtctttca gggaaataag agaatccatt 781 tgataaaatg gccagatgtc tgcaaattga agtttgaagg gaagacattt tatgtgattg 841 gcacccagaa ggagaaaaaa gccatgttgg cattcaatac ttcaacacca gctgcctgca 901 aacatctttg gaagtgtgga gtggaaaacc aggcctttta taagtatgca aaatccagtc 961 agatcaagac tgtatcaagc agcaagatat tttttaaagg aagtagattt cgatatagtg 1021 ggaaagttgc caaagaggtg gtggaggcca gttccaagat ccagagggag cctcctgagg 1081 tgcacagagc caacattact cagagccgca gttcccactc cttgaacaaa cagctcatca 1141 ttaacatgga acccctgcag cccctgcttc cttcccccag cgagcaagaa gaagaacttc 1201 ctctgggtga gggtgttcca ttgcctaaag aggagaacat ttctgctccc ttgatctcca 1261 gctccccagt gaaggcagcc cgggagtatg aagatccccc tagtgaagag gaagataaaa 1321 taaaagaaga acctttaacc atctctgaac tagtgtacaa cccaagtgcc agcctgctcc 1381 ccacccctgt ggatgacgat gagattgaca tgctctttga ctgtccttct aggcttGAGT 1441 TGGAAAGAGA AGACACAGAT TCATTTGAGG ATCTGGAAGC AGATGAAAAC GCCTTTTTGA 1501 TTGCTGAAGA AGAGGAGCTG AAGGAGGCTC GCCGTGCTTT GTCGTGGAGC TACGACATTC 1561 TGACTGGCCA TATTCGGGTG AACCCACTGG TCAAGAGTTT TTCCAGGCTC CTTGTGGTGG 1621 GCCTGGGACT GCTGCTCTTT GTATTTCCCC TGCTCCTCCT CCTTTTGGAG TCAGGTATTG 1681 ATCTCTCCTT CTTATGCGAA ATCCGCCAGA CACCAGAGTT TGAGCAGTTT CACTATGAAT 1741 ACTACTGTCC CCTCAAGGAG TGGGTGGCTG GGAAAGTCCA CCTCATCCTC TACATGCTGG 1801 GTTGCTCATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1861 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1921 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGGTTA ACTCATATGT TAACTCGGAC 1981 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt