Construct: ORF TRCN0000492333
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010677.1_s317c1
- Derived from:
- ccsbBroadEn_07007
- DNA Barcode:
- ACCAGCCGCGCTTCTGCTGAAATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAC (6769)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000492333
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6769 | STAC | SH3 and cysteine rich domain | NM_003149.2 | 99.9% | 99.7% | 333G>T |
| 2 | human | 6769 | STAC | SH3 and cysteine rich domain | XM_011534037.3 | 97.1% | 97% | 107_108ins33;300G>T |
| 3 | human | 6769 | STAC | SH3 and cysteine rich domain | XM_024453722.1 | 91% | 89.6% | (many diffs) |
| 4 | human | 6769 | STAC | SH3 and cysteine rich domain | NM_001292049.1 | 84.7% | 84.5% | 333G>T;387_388ins183 |
| 5 | human | 6769 | STAC | SH3 and cysteine rich domain | XM_011534038.2 | 83% | 82.8% | 0_1ins204;129G>T |
| 6 | human | 6769 | STAC | SH3 and cysteine rich domain | XM_017007083.1 | 83% | 82.8% | 0_1ins204;129G>T |
| 7 | human | 6769 | STAC | SH3 and cysteine rich domain | XM_024453723.1 | 76.5% | 74.8% | (many diffs) |
| 8 | human | 6769 | STAC | SH3 and cysteine rich domain | XR_940496.2 | 37.9% | (many diffs) | |
| 9 | mouse | 20840 | Stac | src homology three (SH3) an... | NM_016853.3 | 84.8% | 82.6% | (many diffs) |
| 10 | mouse | 20840 | Stac | src homology three (SH3) an... | XM_006512016.3 | 82.2% | 79.9% | (many diffs) |
| 11 | mouse | 20840 | Stac | src homology three (SH3) an... | NM_001324546.1 | 56.3% | 56.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1272
- ORF length:
- 1206
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ccctccgagc agcccccgcg aggacggcgt ggacgggctg cccaaggagg 121 cggtgggcgc cgagcaaccg ccctctcctg catccaccag cagccaggaa tccaagctcc 181 agaaactaaa acgatcactt tctttcaaga ccaagagttt acggagcaaa agtgctgaca 241 acttcttcca gcgaaccaac agcgaagaca tgaaactgca agcacacatg gtggctgaga 301 tcagccccag ctccagccca ctccctgctc caggaagcct gacgtccaca cccgccaggg 361 ctggtctgca tccaggtggc aaggctcatg cctttcatga atacatcttc aagaagccca 421 ctttctgtga tgtctgcaac cacatgatag tgggaacaaa tgctaagcat ggactgcgct 481 gcaaagcctg taagatgagc atccaccaca agtgcacaga tggcctggca ccccagcggt 541 gcatgggcaa gctgccaaag gggtttcggc gttactacag ctcccccttg ctcattcatg 601 aacagtttgg ctgcattaaa gaagttatgc ccattgcctg tggcaataag gtggaccctg 661 tctacgagac cctccgcttc ggcacctccc tggcccagag gacaaagaag ggcagctccg 721 gcagtggctc tgactcacct cacagaacct ctacttcaga tcttgtggag gttcctgagg 781 aagccaatgg gccaggaggc gggtatgacc taaggaaacg cagcaacagc gtgtttacat 841 atccagaaaa tggCACTGAT GATTTCAGAG ATCCAGCGAA GAACATAAAC CACCAGGGAT 901 CTCTTTCCAA AGACCCATTA CAGATGAACA CCTATGTTGC CTTGTACAAA TTTGTACCAC 961 AGGAGAATGA AGATTTGGAA ATGAGGCCAG GAGACATAAT TACTCTTTTA GAGGATTCCA 1021 ATGAAGACTG GTGGAAAGGG AAAATTCAAG ACAGAATTGG CTTCTTTCCA GCCAACTTTG 1081 TTCAGAGACT ACAACAAAAT GAGAAGATTT TTAGATGTGT TAGAACCTTC ATTGGGTGTA 1141 AGGAACAGGG GCAGATAACA CTGAAAGAGA ATCAGATCTG CGTGAGTTCT GAAGAAGAAC 1201 AAGATGGTTT TATCAGAGTC CTCAGTGGAA AAAAGAAAGG CCTCATCCCC CTTGATGTAC 1261 TAGAAAACAT CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1321 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1381 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAACC AGCCGCGCTT CTGCTGAAAT 1441 TACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t