Construct: ORF ccsbBroad304_00520
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012678.1_s304c1, BRDN0000411892
- Derived from:
- ccsbBroadEn_00520
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ETV1 (2115)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00520
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2115 | ETV1 | ETS variant transcription f... | NM_001370555.1 | 100% | 100% | |
| 2 | human | 2115 | ETV1 | ETS variant transcription f... | NM_004956.5 | 100% | 100% | |
| 3 | human | 2115 | ETV1 | ETS variant transcription f... | XM_005249635.5 | 100% | 100% | |
| 4 | human | 2115 | ETV1 | ETS variant transcription f... | XM_011515168.3 | 100% | 100% | |
| 5 | human | 2115 | ETV1 | ETS variant transcription f... | XM_011515170.3 | 97.6% | 96.8% | (many diffs) |
| 6 | human | 2115 | ETV1 | ETS variant transcription f... | NM_001370556.1 | 96.6% | 96.6% | 127_128ins48 |
| 7 | human | 2115 | ETV1 | ETS variant transcription f... | NM_001163148.1 | 96.2% | 96% | 181_182ins54 |
| 8 | human | 2115 | ETV1 | ETS variant transcription f... | NM_001163149.1 | 96.2% | 96% | 181_182ins54 |
| 9 | human | 2115 | ETV1 | ETS variant transcription f... | XM_024446677.1 | 96.2% | 96% | 181_182ins54 |
| 10 | human | 2115 | ETV1 | ETS variant transcription f... | XM_024446678.1 | 96.2% | 96% | 181_182ins54 |
| 11 | human | 2115 | ETV1 | ETS variant transcription f... | XM_024446679.1 | 96.2% | 96% | 181_182ins54 |
| 12 | human | 2115 | ETV1 | ETS variant transcription f... | NM_001163147.1 | 95.1% | 95.1% | 800_801ins69 |
| 13 | human | 2115 | ETV1 | ETS variant transcription f... | XM_011515167.3 | 95.1% | 94.1% | (many diffs) |
| 14 | human | 2115 | ETV1 | ETS variant transcription f... | XM_024446676.1 | 91.5% | 90.3% | (many diffs) |
| 15 | human | 2115 | ETV1 | ETS variant transcription f... | NM_001163150.1 | 88.1% | 87.3% | (many diffs) |
| 16 | human | 2115 | ETV1 | ETS variant transcription f... | NM_001163151.1 | 86.6% | 85.3% | (many diffs) |
| 17 | human | 2115 | ETV1 | ETS variant transcription f... | NM_001163152.1 | 75.1% | 74.3% | (many diffs) |
| 18 | human | 2115 | ETV1 | ETS variant transcription f... | NR_120445.1 | 21.7% | 1_434del;1374_1437del;1930_6583del | |
| 19 | mouse | 14009 | Etv1 | ets variant 1 | NM_007960.5 | 93.7% | 97.9% | (many diffs) |
| 20 | mouse | 14009 | Etv1 | ets variant 1 | XM_006514965.2 | 93.7% | 97.9% | (many diffs) |
| 21 | mouse | 14009 | Etv1 | ets variant 1 | NM_001347379.1 | 90% | 93.9% | (many diffs) |
| 22 | mouse | 14009 | Etv1 | ets variant 1 | XM_006514964.3 | 88.5% | 91.7% | (many diffs) |
| 23 | mouse | 14009 | Etv1 | ets variant 1 | XM_006514966.3 | 84.9% | 87.9% | (many diffs) |
| 24 | mouse | 14009 | Etv1 | ets variant 1 | XM_006514970.3 | 82.1% | 85.9% | (many diffs) |
| 25 | mouse | 14009 | Etv1 | ets variant 1 | NM_001163154.1 | 82.1% | 85.7% | (many diffs) |
| 26 | mouse | 14009 | Etv1 | ets variant 1 | XM_006514968.3 | 82.1% | 85.7% | (many diffs) |
| 27 | mouse | 14009 | Etv1 | ets variant 1 | XM_006514971.3 | 80.9% | 83.6% | (many diffs) |
| 28 | mouse | 14009 | Etv1 | ets variant 1 | XM_006514969.3 | 80.8% | 79.7% | (many diffs) |
| 29 | mouse | 14009 | Etv1 | ets variant 1 | XM_006514972.3 | 67.3% | 71.2% | (many diffs) |
| 30 | mouse | 432800 | Gm5454 | predicted gene 5454 | XR_873890.1 | 56% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1500
- ORF length:
- 1431
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatggattt tatgaccagc aagtgcctta catggtcacc aatagtcagc 121 gtgggagaaa ttgtaacgag aaaccaacaa atgtcaggaa aagaaaattc attaacagag 181 atctggctca tgattcagaa gaactctttc aagatctaag tcaattacag gaaacatggc 241 ttgcagaagc tcaggtacct gacaatgatg agcagtttgt accagactat caggctgaaa 301 gtttggcttt tcatggcctg ccactgaaaa tcaagaaaga accccacagt ccatgttcag 361 aaatcagctc tgcctgcagt caagaacagc cctttaaatt cagctatgga gaaaagtgcc 421 tgtacaatgt cagtgcctat gatcagaagc cacaagtggg aatgaggccc tccaaccccc 481 ccacaccatc cagcacgcca gtgtccccac tgcatcatgc atctccaaac tcaactcata 541 caccgaaacc tgaccgggcc ttcccagctc acctccctcc atcgcagtcc ataccagata 601 gcagctaccc catggaccac agatttcgcc gccagctttc tgaaccctgt aactcctttc 661 ctcctttgcc gacgatgcca agggaaggac gtcctatgta ccaacgccag atgtctgagc 721 caaacatccc cttcccacca caaggcttta agcaggagta ccacgaccca gtgtatgaac 781 acaacaccat ggttggcagt gcggccagcc aaagctttcc ccctcctctg atgattaaac 841 aggaacccag agattttgca tatgactcag aagtgcctag ctgccactcc atttatatga 901 ggcaagaagg cttcctggct catcccagca gaacagaagg ctgtatgttt gaaaagggcc 961 ccaggcagtt ttatgatgac acctgtgttg tcccagaaaa attcgatgga gacatcaaac 1021 aagagccagg aatgtatcgg gaaggaccca cataccaacg gcgaggatca cttcagctct 1081 ggcagttttt ggtagctctt ctggatgaCC CTTCAAATTC TCATTTTATT GCCTGGACTG 1141 GTCGAGGCAT GGAATTTAAA CTGATTGAGC CTGAAGAGGT GGCCCGACGT TGGGGCATTC 1201 AGAAAAACAG GCCAGCTATG AACTATGATA AACTTAGCCG TTCACTCCGC TATTACTATG 1261 AGAAAGGAAT TATGCAAAAG GTGGCTGGAG AGAGATATGT CTACAAGTTT GTGTGTGATC 1321 CAGAAGCCCT TTTCTCCATG GCCTTTCCAG ATAATCAGCG TCCACTGCTG AAGACAGACA 1381 TGGAACGTCA CATCAACGAG GAGGACACAG TGCCTCTTTC TCACTTTGAT GAGAGCATGG 1441 CCTACATGCC GGAAGGGGGC TGCTGCAACC CCCACCCCTA CAACGAAGGC TACGTGTATT 1501 TCCCAACTTT CTTGTACaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1561 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1621 ggatta