Construct: ORF ccsbBroad304_00652
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012991.1_s304c1, BRDN0000402387
- Derived from:
- ccsbBroadEn_00652
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNAS (2778)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00652
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2778 | GNAS | GNAS complex locus | NM_001077488.4 | 100% | 100% | |
| 2 | human | 2778 | GNAS | GNAS complex locus | NM_000516.6 | 99.7% | 99.7% | 256_257insGCA |
| 3 | human | 2778 | GNAS | GNAS complex locus | NM_080426.4 | 96.2% | 95.9% | 212_213ins45 |
| 4 | human | 2778 | GNAS | GNAS complex locus | NM_001077489.4 | 95.9% | 95.6% | 212_213ins48 |
| 5 | human | 2778 | GNAS | GNAS complex locus | XM_024451872.1 | 88.5% | 88.1% | (many diffs) |
| 6 | human | 2778 | GNAS | GNAS complex locus | NM_001309840.2 | 84.8% | 84.8% | 0_1ins177;79_80insGCA |
| 7 | human | 2778 | GNAS | GNAS complex locus | NM_001309861.2 | 84.8% | 84.8% | 0_1ins177;79_80insGCA |
| 8 | human | 2778 | GNAS | GNAS complex locus | XM_024451873.1 | 84.8% | 84.8% | 0_1ins177;79_80insGCA |
| 9 | human | 2778 | GNAS | GNAS complex locus | XM_024451874.1 | 84.8% | 84.8% | 0_1ins177;79_80insGCA |
| 10 | human | 2778 | GNAS | GNAS complex locus | XM_024451875.1 | 84.8% | 84.8% | 0_1ins177;79_80insGCA |
| 11 | human | 2778 | GNAS | GNAS complex locus | XM_017027815.1 | 84.7% | 84.1% | (many diffs) |
| 12 | human | 2778 | GNAS | GNAS complex locus | XM_017027817.1 | 81% | 80.7% | 0_1ins177;35_36ins48 |
| 13 | human | 2778 | GNAS | GNAS complex locus | XM_017027818.2 | 81% | 80.7% | 0_1ins177;35_36ins48 |
| 14 | human | 2778 | GNAS | GNAS complex locus | XM_017027819.1 | 81% | 80.7% | 0_1ins177;35_36ins48 |
| 15 | human | 2778 | GNAS | GNAS complex locus | XM_017027816.1 | 80.2% | 78.9% | (many diffs) |
| 16 | human | 2778 | GNAS | GNAS complex locus | XM_017027820.1 | 80.2% | 78.9% | (many diffs) |
| 17 | human | 2778 | GNAS | GNAS complex locus | XR_002958471.1 | 52.5% | (many diffs) | |
| 18 | human | 2778 | GNAS | GNAS complex locus | XM_017027812.2 | 36.8% | 36.1% | (many diffs) |
| 19 | human | 2778 | GNAS | GNAS complex locus | NM_080425.3 | 36.8% | 36% | (many diffs) |
| 20 | human | 2778 | GNAS | GNAS complex locus | XM_017027813.2 | 35.4% | 34.5% | (many diffs) |
| 21 | human | 2778 | GNAS | GNAS complex locus | XM_017027814.2 | 35.3% | 34.4% | (many diffs) |
| 22 | human | 2778 | GNAS | GNAS complex locus | NM_001309842.2 | 21.7% | 21.7% | 258A>C;260_261delTAins926 |
| 23 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | XM_006498774.1 | 94.9% | 99.7% | (many diffs) |
| 24 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | NM_001310083.1 | 94.7% | 99.4% | (many diffs) |
| 25 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | NM_201616.2 | 94.7% | 99.4% | (many diffs) |
| 26 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | NM_001077510.4 | 91.1% | 95.6% | (many diffs) |
| 27 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | XM_006498776.1 | 90.9% | 95.4% | (many diffs) |
| 28 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | XM_006498777.2 | 83.1% | 87.7% | (many diffs) |
| 29 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | XM_017315658.1 | 80.1% | 84.5% | (many diffs) |
| 30 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | XM_006498778.2 | 79.2% | 83.4% | (many diffs) |
| 31 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | NR_003258.1 | 64.6% | (many diffs) | |
| 32 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | NM_010309.4 | 31.9% | 31.9% | (many diffs) |
| 33 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | NM_001077507.2 | 30.6% | 30.6% | (many diffs) |
| 34 | mouse | 14683 | Gnas | GNAS (guanine nucleotide bi... | XM_006498780.1 | 30.6% | 30.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1251
- ORF length:
- 1185
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgcctcggg aacagtaaga ccgaggacca gcgcaacgag gagaaggcgc 121 agcgtgaggc caacaaaaag atcgagaagc agctgcagaa ggacaagcag gtctaccggg 181 ccacgcaccg cctgctgctg ctgggtgctg gagaatctgg taaaagcacc attgtgaagc 241 agatgaggat cctgcatgtt aatgggttta atggagaggg cggcgaagag gacccgcagg 301 ctgcaaggag caacagcgat ggcagtgaga aggcaaccaa agtgcaggac atcaaaaaca 361 acctgaaaga ggcgattgaa accattgtgg ccgccatgag caacctggtg ccccccgtgg 421 agctggccaa ccccgagaac cagttcagag tggactacat cctgagtgtg atgaacgtgc 481 ctgactttga cttccctccc gaattctatg agcatgccaa ggctctgtgg gaggatgaag 541 gagtgcgtgc ctgctacgaa cgctccaacg agtaccagct gattgactgt gcccagtact 601 tcctggacaa gatcgacgtg atcaagcagg ctgactatgt gccgagcgat caggacctgc 661 ttcgctgccg tgtcctgact tctggaatct ttgagaccaa gttccaggtg gacaaagtca 721 acttccacat gtttgacgtg ggtggccagc gcgatgaacg ccgcaagtgg atccagtgct 781 tcaacgatgt gactgccatc atcttcgtgg tggccagcag cagctacaac atggtcatcc 841 gggaggacaa ccagaccaac cgcctgcagg aggctctgaa cctcttcaag agcatctgga 901 acaacagatg gctgcgcacc atctctgtga tcctgttcct caacaagcaa gatctgctcg 961 ctgagaaagt ccttgctggg aaatcgaaga ttgaggacta ctttccagaa tttgctcgct 1021 acactactcc tgaggatgct actcccgagc ccggagagga cccacgcgtg acccgggcca 1081 agtacttcat tcgagatgag tttctgagga tcagcactgc cagtggagat gggcgtcact 1141 actgctaccc tcatttcacc tgcgctgtgg acactgagaa catccgccgt gtgttcaacg 1201 actgccgtga catcattcag cgcatgcacc ttcgtcagta cgagctgctc tacccaactt 1261 tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt 1321 agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta