Transcript: Human XM_017027813.2

PREDICTED: Homo sapiens GNAS complex locus (GNAS), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GNAS (2778)
Length:
3707
CDS:
273..3344

Additional Resources:

NCBI RefSeq record:
XM_017027813.2
NBCI Gene record:
GNAS (2778)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017027813.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000310794 ACCCACCATAGGGCATGATTA pLKO_005 3423 3UTR 100% 13.200 18.480 N GNAS n/a
2 TRCN0000303965 TCACTACTGCTACCCTCATTT pLKO_005 3227 CDS 100% 13.200 18.480 N GNAS n/a
3 TRCN0000083416 CAGAATTTGCTCGCTACACTA pLKO.1 3097 CDS 100% 4.950 6.930 N GNAS n/a
4 TRCN0000300317 CAGAATTTGCTCGCTACACTA pLKO_005 3097 CDS 100% 4.950 6.930 N GNAS n/a
5 TRCN0000369979 TAAAGCCTTAAGCACAATTAA pLKO_005 3367 3UTR 100% 15.000 10.500 N GNAS n/a
6 TRCN0000115056 CCTGCATGTTAATGGGTTTAA pLKO.1 2387 CDS 100% 13.200 9.240 N Gnas n/a
7 TRCN0000320192 CCTGCATGTTAATGGGTTTAA pLKO_005 2387 CDS 100% 13.200 9.240 N Gnas n/a
8 TRCN0000083413 GCTTGCTTAGATGTTCCAAAT pLKO.1 3500 3UTR 100% 10.800 7.560 N GNAS n/a
9 TRCN0000083417 CAACGATGTGACTGCCATCAT pLKO.1 2873 CDS 100% 4.950 3.465 N GNAS n/a
10 TRCN0000331232 CAACGATGTGACTGCCATCAT pLKO_005 2873 CDS 100% 4.950 3.465 N GNAS n/a
11 TRCN0000083414 GCCAAGTACTTCATTCGAGAT pLKO.1 3168 CDS 100% 4.050 2.835 N GNAS n/a
12 TRCN0000300316 GCCAAGTACTTCATTCGAGAT pLKO_005 3168 CDS 100% 4.050 2.835 N GNAS n/a
13 TRCN0000083415 CCTCCCGAATTCTATGAGCAT pLKO.1 2586 CDS 100% 0.000 0.000 N GNAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017027813.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15431 pDONR223 0% 35.4% 34.6% None (many diffs) n/a
2 ccsbBroad304_15431 pLX_304 0% 35.4% 34.6% V5 (many diffs) n/a
3 TRCN0000473616 GACTTATCGCTAGTTCTTCAGTAC pLX_317 47.5% 35.4% 34.6% V5 (many diffs) n/a
4 ccsbBroadEn_00652 pDONR223 100% 35.4% 34.5% None (many diffs) n/a
5 ccsbBroad304_00652 pLX_304 0% 35.4% 34.5% V5 (many diffs) n/a
6 TRCN0000478852 GCCGCACGACTCTCAATGTTCATT pLX_317 29.2% 35.4% 34.5% V5 (many diffs) n/a
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