Construct: ORF ccsbBroad304_00921
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007817.1_s304c1, BRDN0000400020
- Derived from:
- ccsbBroadEn_00921
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT33B (3884)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00921
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3884 | KRT33B | keratin 33B | NM_002279.5 | 100% | 100% | |
| 2 | human | 3883 | KRT33A | keratin 33A | NM_004138.4 | 95.3% | 93.8% | (many diffs) |
| 3 | human | 3881 | KRT31 | keratin 31 | NM_002277.3 | 91.2% | 91.3% | (many diffs) |
| 4 | human | 100653049 | LOC100653049 | keratin, type I cuticular Ha4 | XM_011547005.2 | 85.6% | 84.1% | (many diffs) |
| 5 | human | 3885 | KRT34 | keratin 34 | XM_011524793.2 | 85.6% | 83.8% | (many diffs) |
| 6 | human | 100653049 | LOC100653049 | keratin, type I cuticular Ha4 | XM_003403882.5 | 81% | 79.5% | (many diffs) |
| 7 | human | 3885 | KRT34 | keratin 34 | NM_021013.3 | 81% | 79.3% | (many diffs) |
| 8 | human | 3883 | KRT33A | keratin 33A | XM_011524786.3 | 54.7% | 48.9% | (many diffs) |
| 9 | mouse | 16671 | Krt33b | keratin 33B | NM_013570.1 | 87.5% | 86.8% | (many diffs) |
| 10 | mouse | 71888 | Krt33a | keratin 33A | NM_027983.3 | 87% | 87.8% | (many diffs) |
| 11 | mouse | 16660 | Krt31 | keratin 31 | NM_010659.2 | 84.4% | 85% | (many diffs) |
| 12 | mouse | 16672 | Krt34 | keratin 34 | NM_027563.4 | 83.7% | 84.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1278
- ORF length:
- 1212
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc ctacaacttc tgcctgccca gcctgagctg ccgcaccagc tgctcctccc 121 ggccctgtgt gccccccagc tgccacggct acaccctgcc cggggcctgc aacatccctg 181 ccaatgtgag caactgcaac tggttctgcg agggctcctt caatggcagc gagaaggaga 241 ctatgcagtt cctgaacgac cgcctggcca gctacctgga gaaggtgcgt cagctggagc 301 gggacaacgc ggagctggag aacctcatcc gggagcggtc tcagcagcag gagcccttgc 361 tgtgccccag ctaccagtcc tacttcaaga ccattgagga gctccagcag aagatcctgt 421 gcagcaagtc tgagaatgcc aggctggtgg tgcagatcga caatgccaag ctggctgcag 481 atgacttcag aaccaagtac cagacggagc agtccctgcg gcagctggtg gagtccgaca 541 tcaacagcct gcgcaggatt ctggatgagc tgaccctgtg caggtctgac ctggaggccc 601 agatggagtc cctgaaggag gagctgctgt ccctcaagca gaaccatgag caggaagtca 661 acaccttgcg ctgccagctt ggagaccgcc tcaacgtgga ggtggacgct gctcccgctg 721 tggacctgaa ccaggtcctg aacgagacca ggaatcagta tgaggccctg gtggaaacca 781 accgcaggga agtggagcaa tggttcgcca cgcagaccga ggagctgaac aagcaggtgg 841 tatccagctc ggagcagctg cagtcctacc aggcggagat catcgagctg agacgcacag 901 tcaatgccct ggagatcgag ctgcaggccc agcacaacct gcgatactct ctggaaaaca 961 cgctgacaga gagcgaggcc cgctacagct cccagctgtc ccaggtgcag agcctgatca 1021 ccaacgtgga gtcccagctg gcggagatcc gcagtgacct ggagcggcag aaccaggagt 1081 atcaggtgct gctggacgtg cgggcgcggc tggagtgtga gatcaacaca taccggagcc 1141 tgctggagag cgaggactgc aagctgccct ccaacccctg cgccaccacc aatgcatgtg 1201 aaaagcccat tggatcctgt gtcaccaatc cttgtggtcc tcgttcccgc tgtgggcctt 1261 gcaacacctt tgggtactac ccaactttct tgtacaaagt ggttggtaag cctatcccta 1321 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 1381 agtcgacaat caacctctgg atta