Construct: ORF ccsbBroad304_01249
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000335.1_s304c1, BRDN0000406732
- Derived from:
- ccsbBroadEn_01249
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PPARG (5468)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_01249
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354666.2 | 99.9% | 57.9% | 803delC |
| 2 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354667.2 | 99.9% | 57.9% | 803delC |
| 3 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374263.1 | 99.9% | 57.9% | 803delC |
| 4 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374264.1 | 99.9% | 57.9% | 803delC |
| 5 | human | 5468 | PPARG | peroxisome proliferator act... | NM_005037.6 | 99.9% | 57.9% | 803delC |
| 6 | human | 5468 | PPARG | peroxisome proliferator act... | NM_138711.4 | 99.9% | 57.9% | 803delC |
| 7 | human | 5468 | PPARG | peroxisome proliferator act... | NM_138712.4 | 99.9% | 57.9% | 803delC |
| 8 | human | 5468 | PPARG | peroxisome proliferator act... | NM_015869.5 | 94.3% | 54.7% | 1_84del;887delC |
| 9 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354669.2 | 55.6% | 7.4% | 0_1ins433;102_103ins200;170delC |
| 10 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354670.2 | 54.7% | 80.9% | (many diffs) |
| 11 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001354668.2 | 51.7% | 74.1% | (many diffs) |
| 12 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001330615.2 | 51.7% | 80% | (many diffs) |
| 13 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374261.1 | 51.7% | 80% | (many diffs) |
| 14 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374262.1 | 51.7% | 80% | (many diffs) |
| 15 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374265.1 | 48.8% | 73.3% | (many diffs) |
| 16 | human | 5468 | PPARG | peroxisome proliferator act... | NM_001374266.1 | 46.4% | 72.2% | (many diffs) |
| 17 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_001127330.2 | 90.2% | 56.4% | (many diffs) |
| 18 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_001308352.1 | 90.2% | 56.4% | (many diffs) |
| 19 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_001308354.1 | 90.2% | 56.4% | (many diffs) |
| 20 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505743.3 | 90.2% | 56.4% | (many diffs) |
| 21 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_011241252.1 | 90.2% | 56.4% | (many diffs) |
| 22 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_017321455.1 | 90.2% | 56.4% | (many diffs) |
| 23 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505737.3 | 86.4% | 54% | (many diffs) |
| 24 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505738.3 | 86.4% | 54% | (many diffs) |
| 25 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_006505739.2 | 86.4% | 54% | (many diffs) |
| 26 | mouse | 19016 | Pparg | peroxisome proliferator act... | NM_011146.3 | 85.5% | 53.5% | (many diffs) |
| 27 | mouse | 19016 | Pparg | peroxisome proliferator act... | XR_001785108.1 | 61.6% | (many diffs) | |
| 28 | mouse | 19016 | Pparg | peroxisome proliferator act... | XM_017321456.1 | 55.7% | 19.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 984
- ORF length:
- 918
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac catggttgac acagagatgc cattctggcc caccaacttt gggatcagct 121 ccgtggatct ctccgtaatg gaagaccact cccactcctt tgatatcaag cccttcacta 181 ctgttgactt ctccagcatt tctactccac attacgaaga cattccattc acaagaacag 241 atccagtggt tgcagattac aagtatgacc tgaaacttca agagtaccaa agtgcaatca 301 aagtggagcc tgcatctcca ccttattatt ctgagaagac tcagctctac aataagcctc 361 atgaagagcc ttccaactcc ctcatggcaa ttgaatgtcg tgtctgtgga gataaagctt 421 ctggatttca ctatggagtt catgcttgtg aaggatgcaa gggtttcttc cggagaacaa 481 tcagattgaa gcttatctat gacagatgtg atcttaactg tcggatccac aaaaaaagta 541 gaaataaatg tcagtactgt cggtttcaga aatgccttgc agtggggatg tctcataatg 601 ccatcaggtt tgggcggatg ccacaggccg agaaggagaa gctgttggcg gagatctcca 661 gtgatatcga ccagctgaat ccagagtccg ctgacctccg ggccctggca aaacatttgt 721 atgactcata cataaagtcc ttcccgctga ccaaagcaaa ggcgagggcg atcttgacag 781 gaaagacaac agacaaatca ccattcgtta tctatgacat gaattcctta atgatgggag 841 aaGATAAAAT CAAGTTCAAA CACATCACCC CCTGCAGGAG CAGAGCAAAG AGGTGGCCAT 901 CCGCATCTTT CAGGGCTGCC AGTTTCGCTC CGTGGAGGCT GTGCAGGAGA TCACAGAGTA 961 TGCCAAAAGC ATTCCTGGTT TTGTAAATCT TGACTTGAAC GACCAAGTAA CTCTCCTCAA 1021 ATATGGAGTC CACGAGATCA TTTACACAAT GCTGGCCTCC TTGATGAATA AAGATGGGGT 1081 TCTCATATCC GAGGGCCAAG GCTTCATGAC AAGGGAGTTT CTAAAGAGCC TGCGAAAGCC 1141 TTTTGGTGAC TTTATGGAGC CCAAGTTTGA GTTTGCTGTG AAGTTCAATG CACTGGAATT 1201 AGATGACAGC GACTTGGCAA TATTTATTGC TGTCATTATT CTCAGTGGAG ACCGCCCAGG 1261 TTTGCTGAAT GTGAAGCCCA TTGAAGACAT TCAAGACAAC CTGCTACAAG CCCTGGAGCT 1321 CCAGCTGAAG CTGAACCACC CTGAGTCCTC ACAGCTGTTT GCCAAGCTGC TCCAGAAAAT 1381 GACAGACCTC AGACAGATTG TCACGGAACA CGTGCAGCTA CTGCAGGTGA TCAAGAAGAC 1441 GGAGACAGAC ATGAGTCTTC ACCCGCTCCT GCAGGAGATC TACAAGGACT TGTACTACCC 1501 AACTTTCTTG TACaaagtgg ttggtaagcc tatccctaac cctctcctcg gtctcgattc 1561 tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca acctctggat 1621 ta