Construct: ORF ccsbBroad304_01249

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000335.1_s304c1, BRDN0000406732
Derived from:
ccsbBroadEn_01249
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PPARG (5468)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_01249
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5468 PPARG peroxisome proliferator act... NM_001354666.2 99.9% 57.9% 803delC
2 human 5468 PPARG peroxisome proliferator act... NM_001354667.2 99.9% 57.9% 803delC
3 human 5468 PPARG peroxisome proliferator act... NM_001374263.1 99.9% 57.9% 803delC
4 human 5468 PPARG peroxisome proliferator act... NM_001374264.1 99.9% 57.9% 803delC
5 human 5468 PPARG peroxisome proliferator act... NM_005037.6 99.9% 57.9% 803delC
6 human 5468 PPARG peroxisome proliferator act... NM_138711.4 99.9% 57.9% 803delC
7 human 5468 PPARG peroxisome proliferator act... NM_138712.4 99.9% 57.9% 803delC
8 human 5468 PPARG peroxisome proliferator act... NM_015869.5 94.3% 54.7% 1_84del;887delC
9 human 5468 PPARG peroxisome proliferator act... NM_001354669.2 55.6% 7.4% 0_1ins433;102_103ins200;170delC
10 human 5468 PPARG peroxisome proliferator act... NM_001354670.2 54.7% 80.9% (many diffs)
11 human 5468 PPARG peroxisome proliferator act... NM_001354668.2 51.7% 74.1% (many diffs)
12 human 5468 PPARG peroxisome proliferator act... NM_001330615.2 51.7% 80% (many diffs)
13 human 5468 PPARG peroxisome proliferator act... NM_001374261.1 51.7% 80% (many diffs)
14 human 5468 PPARG peroxisome proliferator act... NM_001374262.1 51.7% 80% (many diffs)
15 human 5468 PPARG peroxisome proliferator act... NM_001374265.1 48.8% 73.3% (many diffs)
16 human 5468 PPARG peroxisome proliferator act... NM_001374266.1 46.4% 72.2% (many diffs)
17 mouse 19016 Pparg peroxisome proliferator act... NM_001127330.2 90.2% 56.4% (many diffs)
18 mouse 19016 Pparg peroxisome proliferator act... NM_001308352.1 90.2% 56.4% (many diffs)
19 mouse 19016 Pparg peroxisome proliferator act... NM_001308354.1 90.2% 56.4% (many diffs)
20 mouse 19016 Pparg peroxisome proliferator act... XM_006505743.3 90.2% 56.4% (many diffs)
21 mouse 19016 Pparg peroxisome proliferator act... XM_011241252.1 90.2% 56.4% (many diffs)
22 mouse 19016 Pparg peroxisome proliferator act... XM_017321455.1 90.2% 56.4% (many diffs)
23 mouse 19016 Pparg peroxisome proliferator act... XM_006505737.3 86.4% 54% (many diffs)
24 mouse 19016 Pparg peroxisome proliferator act... XM_006505738.3 86.4% 54% (many diffs)
25 mouse 19016 Pparg peroxisome proliferator act... XM_006505739.2 86.4% 54% (many diffs)
26 mouse 19016 Pparg peroxisome proliferator act... NM_011146.3 85.5% 53.5% (many diffs)
27 mouse 19016 Pparg peroxisome proliferator act... XR_001785108.1 61.6% (many diffs)
28 mouse 19016 Pparg peroxisome proliferator act... XM_017321456.1 55.7% 19.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
984
ORF length:
918
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgac catggttgac acagagatgc cattctggcc caccaacttt gggatcagct
121ccgtggatct ctccgtaatg gaagaccact cccactcctt tgatatcaag cccttcacta
181ctgttgactt ctccagcatt tctactccac attacgaaga cattccattc acaagaacag
241atccagtggt tgcagattac aagtatgacc tgaaacttca agagtaccaa agtgcaatca
301aagtggagcc tgcatctcca ccttattatt ctgagaagac tcagctctac aataagcctc
361atgaagagcc ttccaactcc ctcatggcaa ttgaatgtcg tgtctgtgga gataaagctt
421ctggatttca ctatggagtt catgcttgtg aaggatgcaa gggtttcttc cggagaacaa
481tcagattgaa gcttatctat gacagatgtg atcttaactg tcggatccac aaaaaaagta
541gaaataaatg tcagtactgt cggtttcaga aatgccttgc agtggggatg tctcataatg
601ccatcaggtt tgggcggatg ccacaggccg agaaggagaa gctgttggcg gagatctcca
661gtgatatcga ccagctgaat ccagagtccg ctgacctccg ggccctggca aaacatttgt
721atgactcata cataaagtcc ttcccgctga ccaaagcaaa ggcgagggcg atcttgacag
781gaaagacaac agacaaatca ccattcgtta tctatgacat gaattcctta atgatgggag
841aaGATAAAAT CAAGTTCAAA CACATCACCC CCTGCAGGAG CAGAGCAAAG AGGTGGCCAT
901CCGCATCTTT CAGGGCTGCC AGTTTCGCTC CGTGGAGGCT GTGCAGGAGA TCACAGAGTA
961TGCCAAAAGC ATTCCTGGTT TTGTAAATCT TGACTTGAAC GACCAAGTAA CTCTCCTCAA
1021ATATGGAGTC CACGAGATCA TTTACACAAT GCTGGCCTCC TTGATGAATA AAGATGGGGT
1081TCTCATATCC GAGGGCCAAG GCTTCATGAC AAGGGAGTTT CTAAAGAGCC TGCGAAAGCC
1141TTTTGGTGAC TTTATGGAGC CCAAGTTTGA GTTTGCTGTG AAGTTCAATG CACTGGAATT
1201AGATGACAGC GACTTGGCAA TATTTATTGC TGTCATTATT CTCAGTGGAG ACCGCCCAGG
1261TTTGCTGAAT GTGAAGCCCA TTGAAGACAT TCAAGACAAC CTGCTACAAG CCCTGGAGCT
1321CCAGCTGAAG CTGAACCACC CTGAGTCCTC ACAGCTGTTT GCCAAGCTGC TCCAGAAAAT
1381GACAGACCTC AGACAGATTG TCACGGAACA CGTGCAGCTA CTGCAGGTGA TCAAGAAGAC
1441GGAGACAGAC ATGAGTCTTC ACCCGCTCCT GCAGGAGATC TACAAGGACT TGTACTACCC
1501AACTTTCTTG TACaaagtgg ttggtaagcc tatccctaac cctctcctcg gtctcgattc
1561tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca acctctggat
1621ta

Download FASTA (ORF) (Full)

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