Transcript: Human NM_138712.4

Homo sapiens peroxisome proliferator activated receptor gamma (PPARG), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-17
Taxon:
Homo sapiens (human)
Gene:
PPARG (5468)
Length:
1846
CDS:
214..1647

Additional Resources:

NCBI RefSeq record:
NM_138712.4
NBCI Gene record:
PPARG (5468)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_138712.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000355862 TCCGTGGATCTCTCCGTAATG pLKO_005 268 CDS 100% 10.800 15.120 N PPARG n/a
2 TRCN0000001672 GAGATCACAGAGTATGCCAAA pLKO.1 1096 CDS 100% 4.050 5.670 N PPARG n/a
3 TRCN0000001671 CTGGCCTCCTTGATGAATAAA pLKO.1 1201 CDS 100% 15.000 10.500 N PPARG n/a
4 TRCN0000355926 ATGGAGTCCACGAGATCATTT pLKO_005 1172 CDS 100% 13.200 9.240 N PPARG n/a
5 TRCN0000001673 CAGCATTTCTACTCCACATTA pLKO.1 342 CDS 100% 13.200 9.240 N PPARG n/a
6 TRCN0000001674 GACAACAGACAAATCACCATT pLKO.1 933 CDS 100% 4.950 3.465 N PPARG n/a
7 TRCN0000001670 GCCAACATTTCCCTTCTTCCA pLKO.1 1667 3UTR 100% 2.640 1.848 N PPARG n/a
8 TRCN0000355861 GACAGCGACTTGGCAATATTT pLKO_005 1354 CDS 100% 15.000 9.000 N PPARG n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_138712.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01249 pDONR223 100% 100% 100% None n/a
2 TRCN0000481296 GTATAACGCCGGAGATCCACGACA pLX_317 29.5% 100% 100% V5 n/a
3 ccsbBroad304_01249 pLX_304 45.7% 99.9% 57.9% V5 (not translated due to prior stop codon) 803delC n/a
4 TRCN0000488857 GGCTGTCCTGGAAACAGACATATG pLX_317 25.8% 99.2% 99.5% V5 (not translated due to prior stop codon) 1_6delATGACC;1431_1432insTAGGC n/a
5 TRCN0000488879 GCAATGGCGCTCTCAGACGTAGTC pLX_317 20.4% 94.3% 94.4% V5 (not translated due to prior stop codon) 0_1ins84;1347C>T n/a
6 TRCN0000489541 AAAGCGGGAAGACGTATTGCCAGG pLX_317 22.5% 94.3% 94.2% V5 (not translated due to prior stop codon) 0_1ins84;733T>G;1347C>T n/a
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