Construct: ORF ccsbBroad304_02053
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013325.1_s304c1, BRDN0000410229
- Derived from:
- ccsbBroadEn_02053
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BTRC (8945)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02053
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 8945 | BTRC | beta-transducin repeat cont... | NM_033637.4 | 100% | 100% | |
2 | human | 8945 | BTRC | beta-transducin repeat cont... | XM_017016871.1 | 97% | 97% | 0_1ins54 |
3 | human | 8945 | BTRC | beta-transducin repeat cont... | XM_017016872.1 | 97% | 97% | 0_1ins54 |
4 | human | 8945 | BTRC | beta-transducin repeat cont... | XM_017016873.2 | 97% | 97% | 0_1ins54 |
5 | human | 8945 | BTRC | beta-transducin repeat cont... | NM_001256856.1 | 95.7% | 95.7% | 153_154ins78 |
6 | human | 8945 | BTRC | beta-transducin repeat cont... | NM_003939.5 | 94% | 94% | 47_48ins108 |
7 | human | 8945 | BTRC | beta-transducin repeat cont... | XM_017016874.1 | 89.7% | 89.7% | 47_48ins186 |
8 | human | 8945 | BTRC | beta-transducin repeat cont... | XM_011540320.2 | 86.2% | 80.1% | (many diffs) |
9 | human | 8945 | BTRC | beta-transducin repeat cont... | XM_024448246.1 | 83.6% | 83.6% | 0_1ins297 |
10 | human | 8945 | BTRC | beta-transducin repeat cont... | XM_024448247.1 | 83.6% | 83.6% | 0_1ins297 |
11 | human | 8945 | BTRC | beta-transducin repeat cont... | XM_006718054.2 | 82.6% | 82.6% | 48_428del |
12 | human | 8945 | BTRC | beta-transducin repeat cont... | XM_017016870.1 | 79% | 79% | 48_428del;534_535ins78 |
13 | human | 8945 | BTRC | beta-transducin repeat cont... | XR_001747256.1 | 26.6% | (many diffs) | |
14 | mouse | 12234 | Btrc | beta-transducin repeat cont... | NM_001037758.2 | 90.8% | 98.6% | (many diffs) |
15 | mouse | 12234 | Btrc | beta-transducin repeat cont... | NM_001286465.1 | 90.8% | 98.6% | (many diffs) |
16 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_006526607.3 | 88.7% | 96.4% | (many diffs) |
17 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_011247120.2 | 88.1% | 95.7% | (many diffs) |
18 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_017318046.1 | 88.1% | 95.7% | (many diffs) |
19 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_006526608.2 | 86.5% | 94.3% | (many diffs) |
20 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_011247122.2 | 86.5% | 92.9% | (many diffs) |
21 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_011247121.2 | 85.1% | 88.4% | (many diffs) |
22 | mouse | 12234 | Btrc | beta-transducin repeat cont... | NM_009771.3 | 84.9% | 92.7% | (many diffs) |
23 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_006526610.3 | 84.5% | 92.2% | (many diffs) |
24 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_011247119.2 | 84.4% | 91.9% | (many diffs) |
25 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_011247123.2 | 82.5% | 88.9% | (many diffs) |
26 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_006526612.2 | 80.6% | 88.4% | (many diffs) |
27 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_006526613.2 | 80.4% | 88% | (many diffs) |
28 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_006526614.3 | 80.2% | 87.7% | (many diffs) |
29 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_006526615.2 | 75.1% | 82.6% | (many diffs) |
30 | mouse | 12234 | Btrc | beta-transducin repeat cont... | XM_011247127.2 | 75.1% | 82.6% | (many diffs) |
31 | mouse | 12234 | Btrc | beta-transducin repeat cont... | NM_001286466.1 | 56.8% | 62.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1881
- ORF length:
- 1815
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga cccggccgag gcggtgctgc aagagaaggc actcaagttt atgtgctcta 121 tgcccaggtc tctgtggctg ggctgctcca gcctggcgga cagcatgcct tcgctgcgat 181 gcctgtataa cccagggact ggcgcactca cagctttcca gaattcctca gagagagaag 241 actgtaataa tggcgaaccc cctaggaaga taataccaga gaagaattca cttagacaga 301 catacaacag ctgtgccaga ctctgcttaa accaagaaac agtatgttta gcaagcactg 361 ctatgaagac tgagaattgt gtggccaaaa caaaacttgc caatggcact tccagtatga 421 ttgtgcccaa gcaacggaaa ctctcagcaa gctatgaaaa ggaaaaggaa ctgtgtgtca 481 aatactttga gcagtggtca gagtcagatc aagtggaatt tgtggaacat cttatatccc 541 aaatgtgtca ttaccaacat gggcacataa actcgtatct taaacctatg ttgcagagag 601 atttcataac tgctctgcca gctcggggat tggatcatat tgctgagaac attctgtcat 661 acctggatgc caaatcacta tgtgctgctg aacttgtgtg caaggaatgg taccgagtga 721 cctctgatgg catgctgtgg aagaagctta tcgagagaat ggtcaggaca gattctctgt 781 ggagaggcct ggcagaacga agaggatggg gacagtattt attcaaaaac aaacctcctg 841 acgggaatgc tcctcccaac tctttttata gagcacttta tcctaaaatt atacaagaca 901 ttgagacaat agaatctaat tggagatgtg gaagacatag tttacagaga attcactgcc 961 gaagtgaaac aagcaaagga gtttactgtt tacagtatga tgatcagaaa atagtaagcg 1021 gccttcgaga caacacaatc aagatctggg ataaaaacac attggaatgc aagcgaattc 1081 tcacaggcca tacaggttca gtcctctgtc tccagtatga tgagagagtg atcataacag 1141 gatcatcgga ttccacggtc agagtgtggg atgtaaatac aggtgaaatg ctaaacacgt 1201 tgattcacca ttgtgaagca gttctgcact tgcgtttcaa taatggcatg atggtgacct 1261 GCTCCAAAGA TCGTTCCATT GCTGTATGGG ATATGGCCTC CCCAACTGAC ATTACCCTCC 1321 GGAGGGTGCT GGTCGGACAC CGAGCTGCTG TCAATGTTGT AGACTTTGAT GACAAGTACA 1381 TTGTTTCTGC ATCTGGGGAT AGAACTATAA AGGTATGGAA CACAAGTACT TGTGAATTTG 1441 TAAGGACCTT AAATGGACAC AAACGAGGCA TTGCCTGTTT GCAGTACAGG GACAGGCTGG 1501 TAGTGAGTGG CTCATCTGAC AACACTATCA GATTATGGGA CATAGAATGT GGTGCATGTT 1561 TACGAGTGTT AGAAGGCCAT GAGGAATTGG TGCGTTGTAT TCGATTTGAT AACAAGAGGA 1621 TAGTCAGTGG GGCCTATGAT GGAAAAATTA AAGTGTGGGA TCTTGTGGCT GCTTTGGACC 1681 CCCGTGCTCC TGCAGGGACA CTCTGTCTAC GGACCCTTGT GGAGCATTCC GGAAGAGTTT 1741 TTCGACTACA GTTTGATGAA TTCCAGATTG TCAGTAGTTC ACATGATGAC ACAATCCTCA 1801 TCTGGGACTT CCTAAATGAT CCAGCTGCCC AAGCTGAACC CCCCCGTTCC CCTTCTCGAA 1861 CATACACCTA CATCTCCAGA TACCCAACTT TCTTGTACaa agtggttggt aagcctatcc 1921 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1981 ttaagtcgac aatcaacctc tggatta