Construct: ORF ccsbBroad304_02087

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015072.1_s304c1, BRDN0000409531
Derived from:
ccsbBroadEn_02087
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PRPF4 (9128)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02087
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9128 PRPF4 pre-mRNA processing factor 4 NM_004697.4 100% 100%
2 human 9128 PRPF4 pre-mRNA processing factor 4 NM_001244926.1 99.8% 99.8% 26_27insGCA
3 human 9128 PRPF4 pre-mRNA processing factor 4 NM_001322266.1 52.5% 52.1% (many diffs)
4 human 9128 PRPF4 pre-mRNA processing factor 4 NM_001322267.1 52.5% 52.1% (many diffs)
5 human 9128 PRPF4 pre-mRNA processing factor 4 NR_136265.1 51.8% 1_110del;1206_1207ins49;1628_2875del
6 human 9128 PRPF4 pre-mRNA processing factor 4 NR_136266.1 51.7% (many diffs)
7 mouse 70052 Prpf4 pre-mRNA processing factor 4 NM_027297.3 90.7% 98.8% (many diffs)
8 mouse 70052 Prpf4 pre-mRNA processing factor 4 XM_006538249.2 48.5% 51.6% (many diffs)
9 mouse 70052 Prpf4 pre-mRNA processing factor 4 XR_390341.1 47.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1632
ORF length:
1566
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ttcctcgcga gcctcttcca cgcaggcaac caaaactaaa gcacccgacg
121acttagttgc tccggtcgtg aagaaaccac acatctatta tggaagtttg gaagagaagg
181agagggagcg tctggccaaa ggagagtctg ggattttggg gaaagacgga cttaaagcag
241ggatcgaagc tggaaatatt aatataacct ctggagaagt gtttgaaatt gaagagcata
301tcagcgagcg acaggcagaa gtattggctg agtttgagag aaggaagcga gcccggcaga
361tcaatgtttc cacagatgac tcagaggtca aagcttgcct tagagccttg ggggaaccca
421tcacactttt tggagagggt cctgctgaaa gaagagaaag gttaagaaat atcctctcag
481ttgtcggtac tgatgccttg aaaaagacca aaaaggatga tgagaagtct aaaaagtcca
541aagaagagta tcagcaaacc tggtatcatg aaggaccaaa tagcttgaag gtggcaagac
601tatggattgc taattattcg ttgcccaggg caatgaaacg cttggaagag gcccgactcc
661ataaggagat tcctgagaca acaaggacct cccagatgca agagctgcac aagtctctcc
721ggtctttgaa taatttttgc agtcagattg gggatgatcg gcctatctcc tactgtcact
781ttagtcccaa ttccaagatg ctggccacag cttgttggag tgggctttgc aagctctggt
841ctgttcctga ttgcaacctc cttcacactc ttcgagggca taacacaaat gtaggagcaa
901ttgtattcca tcccaaatcc actgtctcct tggacccaaa agatgtcaac ctggcctctt
961gtgcggctga tggctctgtg aagctttgga gtctcgacag tgatgaacca gtggcagata
1021ttgaaggcca tacagtgcgt gtggcgcggg taatgtggca tccttcagga cgtttcctgg
1081gcaccacctg ctatgaccgt tcatggcgct tatgggattt ggaggctcaa gaggagatcc
1141tgcatcagga aggccatagc atgggtgtgt atgacattgc cttccatcaa gatggctctt
1201tggctggcac tgggggactg gatgcatttg gtcgagtttg ggacctacgc acaggacgtt
1261gtatcatgtt cttagaaggc cacctgaaag aaatctatgg aataaatttc tcccccaatg
1321gctatcacat tgcaaccggc agtggtgaca acacctgcaa agtgtgggac ctccgacagc
1381ggcgttgcgt ctacaccatc cctgctcatc agaacttagt gactggtgtc aagtttgagc
1441ctatccatgg gaacttcttg cttactggtg cctatgataa cacagccaag atctggacgc
1501acccaggctg gtccccgctg aagactctgg ctggccacga aggcaaagtg atgggcctag
1561atatttcttc cgatgggcag ctcatagcca cttgctcata tgacaggacc ttcaagctgt
1621ggatggctga atacccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
1681tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
1741caatcaacct ctggatta

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