Construct: ORF ccsbBroad304_02428
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016103.1_s304c1, BRDN0000410677
- Derived from:
- ccsbBroadEn_02428
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TIMM23B (653252)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02428
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100287932 | TIMM23 | translocase of inner mitoch... | NM_006327.4 | 100% | 100% | |
| 2 | human | 100652748 | TIMM23B | translocase of inner mitoch... | NM_001290117.1 | 86.2% | 80.8% | (many diffs) |
| 3 | human | 100652748 | TIMM23B | translocase of inner mitoch... | NM_001290118.1 | 63.6% | 58.3% | (many diffs) |
| 4 | human | 100652748 | TIMM23B | translocase of inner mitoch... | NM_001365871.1 | 47.2% | 43% | (many diffs) |
| 5 | human | 100287932 | TIMM23 | translocase of inner mitoch... | NR_073029.1 | 46% | 1_172del;685_808del;924_1363del | |
| 6 | human | 100287932 | TIMM23 | translocase of inner mitoch... | NR_073030.1 | 45.8% | 1_172del;278_279ins59;741_1180del | |
| 7 | human | 100652748 | TIMM23B | translocase of inner mitoch... | NR_110767.1 | 21.7% | (many diffs) | |
| 8 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158656.1 | 19.6% | (many diffs) | |
| 9 | human | 100652748 | TIMM23B | translocase of inner mitoch... | NR_158651.1 | 19.2% | (many diffs) | |
| 10 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158657.1 | 18.8% | (many diffs) | |
| 11 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158654.1 | 17.6% | (many diffs) | |
| 12 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158660.1 | 17.4% | (many diffs) | |
| 13 | human | 100652748 | TIMM23B | translocase of inner mitoch... | NR_158652.1 | 17.2% | (many diffs) | |
| 14 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158659.1 | 16.6% | (many diffs) | |
| 15 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158658.1 | 16.3% | (many diffs) | |
| 16 | human | 113218477 | TIMM23B-AGAP6 | TIMM23B-AGAP6 readthrough | NR_158661.1 | 15.2% | (many diffs) | |
| 17 | mouse | 53600 | Timm23 | translocase of inner mitoch... | NM_016897.3 | 90.6% | 97.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 693
- ORF length:
- 627
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga aggaggcggg ggaagcggca acaaaaccac agggggattg gccggctttt 121 tcggagccgg cggagcaggt tactcgcacg cggatttggc tggcgtcccg ctaactggta 181 tgaaccctct gtctccttat ttaaatgtgg atccacgata cctcgtgcag gatacagatg 241 agtttatttt acctaccgga gctaataaaa cccggggcag atttgagctg gccttcttta 301 cgattggagg atgttgcatg acaggggctg cgtttggtgc aatgaatggt cttcggctag 361 gattgaagga aacccagaac atggcctggt ccaaaccaag aaatgtacag attttgaata 421 tggtgactag gcaaggggca ctttgggcta atactctagg ttctctggct ttgctctata 481 gtgcatttgg tgtcatcatt gagaaaacac gaggtgcaga agatgacctt aacacagtag 541 cagctggaac catgacaggc atgttgtata aatgtacagg tggtcttcga gggatagcac 601 gaggtggtct gacaggacta acacttacca gcctctatgc actatataat aactgggagc 661 acatgaaagg ctccttgctc caacagtcac tctgcccaac tttcttgtac aaagtggttg 721 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 781 accggtggcg cgttaagtcg acaatcaacc tctggatta