Transcript: Human NR_073029.1

Homo sapiens translocase of inner mitochondrial membrane 23 (TIMM23), transcript variant 2, non-coding RNA.

Source:
NCBI, updated 2019-02-23
Taxon:
Homo sapiens (human)
Gene:
TIMM23 (100287932)
Length:
1363
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_073029.1
NBCI Gene record:
TIMM23 (100287932)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_073029.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000233016 CCAGCCTCTATGCACTATATA pLKO_005 859 3UTR 100% 15.000 21.000 N TIMM23 n/a
2 TRCN0000233015 GGTCTGACAGGACTAACACTT pLKO_005 837 3UTR 100% 4.950 6.930 N TIMM23 n/a
3 TRCN0000233018 CTTCTACCTACAATTAGTTTG pLKO_005 1008 3UTR 100% 10.800 7.560 N TIMM23 n/a
4 TRCN0000233017 GAGCACATGAAAGGCTCCTTG pLKO_005 888 3UTR 100% 4.050 2.835 N TIMM23 n/a
5 TRCN0000114247 GCCTGGTCCAAACCAAGAAAT pLKO.1 491 3UTR 100% 13.200 7.920 N Timm23 n/a
6 TRCN0000180093 CCTTTGGTGACTCACTGAGTA pLKO.1 1123 3UTR 100% 4.950 2.970 N TIMM23 n/a
7 TRCN0000178920 CCTTCTTTACGATTGGAGGAT pLKO.1 399 3UTR 100% 2.640 1.320 Y TIMM23 n/a
8 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 752 3UTR 100% 5.625 2.813 Y KLHL30 n/a
9 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 752 3UTR 100% 5.625 2.813 Y EID2B n/a
10 TRCN0000164834 CAAACTCCTGAGCTCAAGCAA pLKO.1 721 3UTR 100% 3.000 1.500 Y LINC00336 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_073029.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02428 pDONR223 100% 46% None 1_172del;685_808del;924_1363del n/a
2 ccsbBroad304_02428 pLX_304 0% 46% V5 1_172del;685_808del;924_1363del n/a
3 ccsbBroadEn_11498 pDONR223 100% 45.7% None (many diffs) n/a
4 ccsbBroad304_11498 pLX_304 0% 45.7% V5 (many diffs) n/a
5 TRCN0000471893 GGGCTAAATTTTAGCTTGGTCAGC pLX_317 74.2% 45.7% V5 (many diffs) n/a
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