Construct: ORF ccsbBroad304_02519
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008287.1_s304c1, BRDN0000403731
- Derived from:
- ccsbBroadEn_02519
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KIF1C (10749)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02519
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10749 | KIF1C | kinesin family member 1C | NM_006612.6 | 100% | 100% | |
| 2 | human | 10749 | KIF1C | kinesin family member 1C | XM_005256424.2 | 100% | 100% | |
| 3 | mouse | 16562 | Kif1c | kinesin family member 1C | NM_153103.2 | 88.2% | 94.1% | (many diffs) |
| 4 | mouse | 16562 | Kif1c | kinesin family member 1C | XM_006532320.3 | 88.2% | 94.1% | (many diffs) |
| 5 | mouse | 16562 | Kif1c | kinesin family member 1C | XM_006532321.3 | 88.2% | 94.1% | (many diffs) |
| 6 | mouse | 16562 | Kif1c | kinesin family member 1C | XM_006532322.3 | 88.2% | 94.1% | (many diffs) |
| 7 | mouse | 16562 | Kif1c | kinesin family member 1C | XM_006532323.3 | 45.2% | 48.8% | (many diffs) |
| 8 | mouse | 16562 | Kif1c | kinesin family member 1C | XM_006532324.2 | 44.1% | 45.8% | (many diffs) |
| 9 | mouse | 16562 | Kif1c | kinesin family member 1C | XR_388354.3 | 41.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3375
- ORF length:
- 3309
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc tggtgcctcg gtgaaagtgg cagtgagggt tcggcccttt aacgcccgtg 121 agaccagcca ggatgccaag tgtgtggtca gcatgcaggg caacaccacc tccatcatca 181 atcctaaaca gagcaaggat gcccccaaaa gcttcacctt tgactactcc tactggtcac 241 acacttcgac ggaggacccc cagtttgcat ctcagcagca agtgtatcgg gacattggag 301 aagagatgct gctccacgcc tttgaaggct acaacgtgtg catctttgcc tatgggcaga 361 ccggggctgg gaaatcctat accatgatgg ggcgacagga gccagggcag cagggcatcg 421 tgccccagct ctgtgaggac ctcttctctc gcgttagtga gaaccagagt gctcagctat 481 cctactctgt ggaggtgagc tatatggaga tctactgtga gcgggtacga gacctcttga 541 accccaagag tcggggttct ctgcgggtcc gggagcaccc catcctgggc ccgtacgtgc 601 aggacctgtc caaattggct gtgacctcct acgcagacat tgctgacctc atggactgtg 661 gaaataaagc acggactgtg gctgccacca acatgaatga gaccagcagc cgttcccatg 721 ccgtctttac catcgtcttc acacagcgct gccatgacca gctcacgggg ctggactcgg 781 agaaggtcag taagatcagt ttggtggacc ttgctgggag tgagcgagcc gactcctcag 841 gggcccgggg catgcgcctg aaggaaggag ccaacatcaa taagtccctg actacactag 901 ggaaagtgat ctcggccctt gcagatatgc aatcaaagaa gcgaaagtcg gattttatcc 961 cctacaggga ctctgtgctc acctggctgc tcaaggaaaa tttggggggg aactcacgca 1021 cagccatgat tgcagccctg agccctgctg acatcaatta cgaggagact ctcagcaccc 1081 tcaggtatgc tgaccgcacc aagcaaatcc gctgcaatgc catcatcaac gaggacccta 1141 atgcccggct gattagagag ctgcaggagg aagtagcccg gctgcgggaa ctgctgatgg 1201 ctcagggact gtcagcctct gctctggaag gcctgaagac ggaagaaggg agtgtcagag 1261 gcgccctgcc agctgtgtca tctcccccag ctccagtttc accctcatca cccaccacac 1321 ataatgggga gctggagccg tcattctccc ccaacacgga gtcccagatt gggcctgagg 1381 aagccatgga gaggctgcag gagacagaga agattatagc tgagctgaac gagacatggg 1441 aggagaagct acgcaagaca gaagccctga ggatggagag agaagcattg ctggctgaga 1501 tgggggtggc cgtccgggag gatgggggaa ctgtgggcgt cttctctcca aagaagactc 1561 cccacctggt gaacctgaac gaagaccctc tgatgtctga gtgtctgctc taccacatca 1621 aagatggcgt caccagggtc ggccaagtag atatggacat caagctgacc ggacagttca 1681 ttcgggagca acactgtctg ttccggagca tcccccagcc agatggagaa gtggtggtca 1741 ctctggagcc ttgtgaagga gctgagacat atgtgaatgg gaagcttgtg acggagccgc 1801 tggtgctgaa gtcagggaat aggattgtga tgggcaagaa ccacgttttc cgcttcaacc 1861 acccggagca ggcaaggctg gaacgggaac gaggggtccc cccaccccca ggaccgccct 1921 ctgagccagt cgactggaac tttgcccaga aggaactgct ggagcagcaa ggcatcgaca 1981 taaagctgga aatggagaag aggctgcagg atctggagaa tcagtaccgg aaagaaaagg 2041 aagaagccga tcttctgctg gagcagcagc gactgtatgc agactcggac agcggggatg 2101 actctgacaa gcgctcttgt gaagagagct ggaggctcat ctcctccttg cgggagcagc 2161 tgccgcccac cacggtccag accattgtca aacgctgtgg tctgcccagc agtggcaagc 2221 gcagggcccc tcgcagggtt tatcagatcc cccagcgacg caggctgcag ggcaaagacc 2281 cccgctgggc caccatggct gacctgaaga tgcaggcggt gaaggagatc tgctacgagg 2341 tggccctggc tgacttccgc cacgggcggg ctgagattga ggccctggcc gccctcaaga 2401 tgcgggagct gtgtcgcacc tatggcaagc cagacggccc cggagacgcc tggagggctg 2461 tggcccggga tgtctgggac actgtaggcg aggaggaagg aggtggagct ggcagtggtg 2521 gtggcagtga ggagggagcc cgaggggcgg aggtggagga cctccgggcc cacatcgaca 2581 agctgacggg gattctgcag gaggtgaagc tgcagaacag cagcaaggac cgggagctgc 2641 aggccctgcg ggaccgcatg ctccgcatgg agagggtcat ccccctggcc caggatcatg 2701 aggatgagaa tgaagaaggt ggtgaggtcc cctgggcccc gcctgaagga tcagaggcag 2761 cagaggaggc agcccccagt gaccgcatgc cgtcagcccg gcccccctcg ccaccactgt 2821 caagctggga gcgggtgtca cggctcatgg aggaggaccc tgccttccgt cgtggtcgtc 2881 ttcgctggct caagcaggag cagctacggc tgcagggact gcagggctct gggggccggg 2941 gcggggggct gcgcaggccc ccagcccgct ttgtgccccc tcacgactgc aagctacgct 3001 tccccttcaa gagcaacccc cagcaccggg agtcttggcc agggatgggg agcggggagg 3061 ctccaactcc gctccaaccc cctgaggagg tcactcccca tccagccacc cctgcccgcc 3121 ggcctccgag tccccgaagg tcccaccatc cccgcaggaa ctccctggat ggagggggcc 3181 gatcccgggg agcgggttct gcacagcctg aaccccagca cttccagccc aaaaagcaca 3241 actcttatcc ccagccaccc caaccctacc cagcccagcg gcccccaggg ccccgctacc 3301 ccccatacac tactccccca cgaatgagac ggcagcgttc tgcccctgac ctcaaggaga 3361 gtggggcagc tgtgtgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 3421 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 3481 cgacaatcaa cctctggatt a