Construct: ORF ccsbBroad304_02720
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003288.1_s304c1, BRDN0000410728
- Derived from:
- ccsbBroadEn_02720
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PEG10 (23089)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02720
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23089 | PEG10 | paternally expressed 10 | NM_001040152.2 | 100% | 100% | |
| 2 | human | 23089 | PEG10 | paternally expressed 10 | NM_001184962.2 | 90.5% | 90.5% | 1_102del |
| 3 | human | 23089 | PEG10 | paternally expressed 10 | NM_001172438.3 | 81% | 81% | 1_228del |
| 4 | human | 23089 | PEG10 | paternally expressed 10 | NM_015068.3 | 45.9% | 100% | 976_2123del |
| 5 | human | 23089 | PEG10 | paternally expressed 10 | NM_001184961.1 | 43.8% | 90.5% | 1_102del;1078_2225del |
| 6 | human | 23089 | PEG10 | paternally expressed 10 | NM_001172437.2 | 41.4% | 81% | 1_228del;1204_2351del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1041
- ORF length:
- 975
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac cgaacgaaga agggacgagc tctctgaaga gatcaacaac ttaagagaga 121 aggtcatgaa gcagtcggag gagaacaaca acctgcagag ccaggtgcag aagctcacag 181 aggagaacac cacccttcga gagcaagtgg aacccacccc tgaggatgag gatgatgaca 241 tcgagctccg cggtgctgca gcagctgctg ccccaccccc tccaatagag gaagagtgcc 301 cagaagacct cccagagaag ttcgatggca acccagacat gctggctcct ttcatggccc 361 agtgccagat cttcatggaa aagagcacca gggatttctc agttgatcgt gtccgtgtct 421 gcttcgtgac aagcatgatg accggccgtg ctgcccgttg ggcctcagca aagctggagc 481 gctcccacta cctgatgcac aactacccag ctttcatgat ggaaatgaag catgtctttg 541 aagaccctca gaggcgagag gttgccaaac gcaagatcag acgcctgcgc caaggcatgg 601 ggtctgtcat cgactactcc aatgctttcc agatgattgc ccaggacctg gattggaacg 661 agcctgcgct gattgaccag taccacgagg gcctcagcga ccacattcag gaggagctct 721 cccacctcga ggtcgccaag tcgctgtctg ctctgattgg gcagtgcatt cacattgaga 781 gaaggctggc cagggctgct gcagctcgca agccacgctc gccaccccgg gcgctggtgt 841 tgcctcacat tgcaagccac caccaggtag atccaaccga gccggtggga ggtgcccgca 901 tgcgcctgac gcaggaagaa aaagaaagac gcagaaagct gaacctgtgc ctctactgtg 961 gaacaggagg tcactacgct gacaattgtc ctgccaaggc ctcaaagtct tcgccggcgg 1021 gaaactcccc ggccccgctg tacccaactt tcttgtacaa agtggttggt aagcctatcc 1081 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1141 ttaagtcgac aatcaacctc tggatta